Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
March 2013 Volume 29 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March 2013 Volume 29 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

FAM172A induces S phase arrest of HepG2 cells via Notch 3

Retraction in: /10.3892/or.2023.8554
  • Authors:
    • Zhiqiang Feng
    • Hongqi Li
    • Shunai Liu
    • Jun Cheng
    • Guoan Xiang
    • Jinqian Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, Air Force General Hospital of PLA, Beijing 100142, P.R. China, Department of Radiation Oncology, Air Force General Hospital of PLA, Beijing 100142, P.R. China, Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, P.R. China, Department of General Surgery, The Second People's Hospital of Guangdong Province, Guangzhou 510515, P.R. China
  • Pages: 1154-1160
    |
    Published online on: January 11, 2013
       https://doi.org/10.3892/or.2013.2235
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Our previous results revealed that FAM172A was significantly downregulated in liver tissue from hepatocellular carcinoma or cirrhotic patients. The present study was designed to elucidate the regulatory role of FAM172A in HepG2 cells. In order to determine the expression of the FAM172A protein, western blot analysis was performed. Confocal laser scanning technique was used to observe the localization of FAM172A in HepG2 cells. Surface plasmon resonance experiments were used to determine the binding activity of FAM172A and active single sugar and Ca2+. The cell cycle progression of HepG2 cells was assessed by flow cytometry. The FAM172A protein was localized in the endoplasmic reticulum of HepG2 cells. This protein was moderately expressed in normal liver tissue, but was significantly decreased in liver tissue of patients with chronic hepatitis B When co-cultured with the FAM172A recombinant protein, HepG2 cells exhibited complete cell cycle arrest in the S phase at a high concentration (100 ng/ml). Proliferation of HepG2 cells treated with the FAM172A recombinant protein was prominently inhibited compared with that of the control cells. Western blot analysis showed that upregulation of Notch 3 and cyclin E may be related with the cell cycle control. Our results indicate that FAM172A may be a novel tumor-suppressor gene, which plays an important role in cell cycle control and tumor cell proliferation. G1/S phase arrest may be mediated, at least partially, by the Notch 3 signaling pathway.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Beasley RP: Hepatitis B virus. The major etiology of hepatocellular carcinoma. Cancer. 61:1942–1956. 1998. View Article : Google Scholar

2 

Papatheodoridis GV, Lampertico P, Manolakopoulos S and Lok A: Incidence of hepatocellular carcinoma in chronic hepatitis B patients receiving nucleos(t)ide therapy: a systematic review. J Hepatol. 53:348–356. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Fan X, Fang D, Bin D, Xueyong L, Jun C and Hongshan W: Serum levels of surface large envelope protein: prognostic marker for hepatitis B e antigen-negative patients with adefovir dipivoxil treatment. Antivir Ther. 14:1149–1156. 2009. View Article : Google Scholar

4 

Wong DK, Yuen MF, Poon RT, Yuen JC, Fung J and Lai CL: Quantification of hepatitis B virus covalently closed circular DNA in patients with hepatocellular carcinoma. J Hepatol. 45:553–559. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Barone M, Daniela Spano D, D'Apolito M, Centra M, Lasalandra C, Capasso M, Di Leo A, Volinia S, Arcelli D, Rosso N, Francavilla A, Tiribelli C and Iolascon A: Gene expression analysis in HBV transgenic mouse liver: a model to study early events related to hepatocarcinogenesis. Mol Med. 12:115–123. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Ehebauer M, Hayward P and Arias AM: Notch, a universal arbiter of cell fate decisions. Science. 314:1414–1415. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Louis AA, Van Eyken P, Haber BA, Hicks C, Weinmaster G, Taub R and Rand EB: Hepatic jagged1 expression studies. Hepatology. 30:1269–1275. 1999. View Article : Google Scholar : PubMed/NCBI

8 

Geisler F, Nagl F, Mazur PK, Lee M, Zimber-Strobl U, Strobl LJ, Radtke F, Schmid RM and Siveke JT: Liver-specific inactivation of Notch2, but not Notch1, compromises intrahepatic bile duct development in mice. Hepatology. 48:607–616. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Yuan ZR, Kobayashi N and Kohsaka T: Human Jagged 1 mutants cause liver defect in Alagille syndrome by overexpression of hepatocyte growth factor. J Mol Biol. 356:559–568. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Nijjar SS, Wallace L, Crosby HA, Hubscher SG and Strain AJ: Altered Notch ligand expression in human liver disease: further evidence for a role of the Notch signaling pathway in hepatic neovascularization and biliary ductular defects. Am J Pathol. 160:1695–1703. 2002. View Article : Google Scholar

11 

Gramantieri L, Giovannini C, Lanzi A, Chieco P, Ravaioli M, Venturi A, Grazi GL and Bolondi L: Aberrant Notch3 and Notch4 expression in human hepatocellular carcinoma. Liver Int. 27:997–1007. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Siegel AB, Olsen SK, Magun A and Brown RS Jr: Sorafenib: where do we go from here? Hepatology. 52:360–369. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Li GL, Wei HS, Song SJ, Guo J and Cheng J: The effects of angiotensin II on gene expression of hepatic stellate cells. Zhonghua Gan Zang Bing Za Zhi. 14:914–919. 2006.(In Chinese).

14 

Gutiérrez Gallego R, Haseley SR, van Miegem VF, Vliegenthart JF and Kamerling JP: Identification of carbohydrates binding to lectins by using surface plasmon resonance in combination with HPLC profiling. Glycobiology. 14:373–386. 2004.PubMed/NCBI

15 

Chen X, Kintner DB, Luo J, Baba A, Matsuda T and Sun D: Endoplasmic reticulum Ca2+ dysregulation and endoplasmic reticulum stress following in vitro neuronal ischemia: role of Na+-K+-Cl− cotransporter. J Neurochem. 106:1563–1576. 2008.

16 

Shuda M, Kondoh N, Imazeki N, Tanaka K, Okada T, Mori K, Hada A, Arai M, Wakatsuki T, Matsubara O, Yamamoto N and Yamamoto M: Activation of the ATF6, XBP1 and grp78 genes in human hepatocellular carcinoma: a possible involvement of the ER stress pathway in hepatocarcinogenesis. J Hepatol. 38:605–614. 2003. View Article : Google Scholar : PubMed/NCBI

17 

Arai M, Kondoh N, Imazeki N, Hada A, Hatsuse K, Kimura F, Matsubara O, Mori K, Wakatsuki T and Yamamoto M: Transformation-associated gene regulation by ATF6alpha during hepatocarcinogenesis. FEBS Lett. 580:184–190. 2006. View Article : Google Scholar : PubMed/NCBI

18 

Gao J, Song Z, Chen Y, Xia L, Wang J, Fan R, Du R, Zhang F, Hong L, Song J, Zou X, Xu H, Zheng G, Liu J and Fan D: Deregulated expression of Notch receptors in human hepatocellular carcinoma. Dig Liver Dis. 40:114–121. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Giovannini C, Lacchini M, Gramantieri L, Chieco P and Bolondi L: Notch3 intracellular domain accumulates in HepG2 cell line. Anticancer Res. 26:2123–2127. 2006.PubMed/NCBI

20 

Xu HY, Li BJ, Wang RF and Andersson R: Alterations of Notch/Jagged mRNA and protein expression after partial hepatectomy in rats. Scand J Gastroenterol. 43:1522–1528. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Monsalve E, Ruiz-García A, Baladrón V, Ruiz-Hidalgo MJ, Sánchez-Solana B, Rivero S, García-Ramírez JJ, Rubio A, Laborda J and Díaz-Guerra MJ: Notch1 upregulates LPS-induced macrophage activation by increasing NF-kappaB activity. Eur J Immunol. 39:2556–2570. 2009. View Article : Google Scholar : PubMed/NCBI

22 

Itoh M, Fu L and Tohda S: NF-κB activation induced by Notch ligand stimulation in acute myeloid leukemia cells. Oncol Rep. 22:631–634. 2009.

23 

Nevzorova YA, Tschaharganeh D, Gassler N, Geng Y, Weiskirchen R, Sicinski P, Trautwein C and Liedtke C: Aberrant cell cycle progression and endoreplication in regenerating livers of mice that lack a single E-type cyclin. Gastroenterology. 137:691–703. 2009. View Article : Google Scholar : PubMed/NCBI

24 

Cho JW, Lee KS and Kim CW: Curcumin attenuates the expression of IL-1β, IL-6, and TNF-α as well as cyclin E in TNF-α-treated HaCaT cells; NF-κB and MAPKs as potential upstream targets. Int J Mol Med. 19:469–474. 2007.

25 

Morga E, Mouad-Amazzal L, Felten P, Heurtaux T, Moro M, Michelucci A, Gabel S, Grandbarbe L and Heuschling P: Jagged1 regulates the activation of astrocytes via modulation of NFkappaB and JAK/STAT/SOCS pathways. Glia. 57:1741–1753. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Feng B, Cheng S, Hsia CY, King LB, Monroe JG and Liou HC: NF-kappaB inducible genes BCL-X and cyclin E promote immature B-cell proliferation and survival. Cell Immunol. 232:9–20. 2004. View Article : Google Scholar : PubMed/NCBI

27 

Bharti AC, Takada Y, Shishodia S and Aggarwal BB: Evidence that receptor activator of nuclear factor (NF)-kappaB ligand can suppress cell proliferation and induce apoptosis through activation of a NF-kappaB-independent and TRAF6-dependent mechanism. J Biol Chem. 279:6065–6076. 2004. View Article : Google Scholar

28 

Chen E and Li CC: Association of Cdk2/cyclin E and NF-kappa B complexes at G1/S phase. Biochem Biophys Res Commun. 249:728–734. 1998. View Article : Google Scholar : PubMed/NCBI

29 

Pujol MJ, Jaime M, Serratosa J, Jaumot M, Agell N and Bachs O: Differential association of p21Cip1 and p27Kip1 with cyclin E-CDK2 during rat liver regeneration. J Hepatol. 33:266–274. 2000. View Article : Google Scholar : PubMed/NCBI

30 

Koroxenidou L, Ohlson LC and Porsch Hällström I: Long-term 17alpha-ethinyl estradiol treatment decreases cyclin E and cdk2 expression, reduces cdk2 kinase activity and inhibits S phase entry in regenerating rat liver. J Hepatol. 43:478–484. 2005. View Article : Google Scholar : PubMed/NCBI

31 

Lin SS, Huang HP, Yang JS, Wu JY, Hsia TC, Lin CC, Lin CW, Kuo CL, Gibson Wood W and Chung JG: DNA damage and endoplasmic reticulum stress mediated curcumin-induced cell cycle arrest and apoptosis in human lung carcinoma A-549 cells through the activation of caspase cascade- and mitochondrial-dependent pathway. Cancer Lett. 272:77–90. 2008. View Article : Google Scholar

32 

Derkx PM and Madrid SM: The foldase CYPB is a component of the secretory pathway of Aspergillus niger and contains the endoplasmic reticulum retention signal HEEL. Mol Genet Genomics. 266:537–545. 2001. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Feng Z, Li H, Liu S, Cheng J, Xiang G and Zhang J: FAM172A induces S phase arrest of HepG2 cells via Notch 3 Retraction in /10.3892/or.2023.8554. Oncol Rep 29: 1154-1160, 2013.
APA
Feng, Z., Li, H., Liu, S., Cheng, J., Xiang, G., & Zhang, J. (2013). FAM172A induces S phase arrest of HepG2 cells via Notch 3 Retraction in /10.3892/or.2023.8554. Oncology Reports, 29, 1154-1160. https://doi.org/10.3892/or.2013.2235
MLA
Feng, Z., Li, H., Liu, S., Cheng, J., Xiang, G., Zhang, J."FAM172A induces S phase arrest of HepG2 cells via Notch 3 Retraction in /10.3892/or.2023.8554". Oncology Reports 29.3 (2013): 1154-1160.
Chicago
Feng, Z., Li, H., Liu, S., Cheng, J., Xiang, G., Zhang, J."FAM172A induces S phase arrest of HepG2 cells via Notch 3 Retraction in /10.3892/or.2023.8554". Oncology Reports 29, no. 3 (2013): 1154-1160. https://doi.org/10.3892/or.2013.2235
Copy and paste a formatted citation
x
Spandidos Publications style
Feng Z, Li H, Liu S, Cheng J, Xiang G and Zhang J: FAM172A induces S phase arrest of HepG2 cells via Notch 3 Retraction in /10.3892/or.2023.8554. Oncol Rep 29: 1154-1160, 2013.
APA
Feng, Z., Li, H., Liu, S., Cheng, J., Xiang, G., & Zhang, J. (2013). FAM172A induces S phase arrest of HepG2 cells via Notch 3 Retraction in /10.3892/or.2023.8554. Oncology Reports, 29, 1154-1160. https://doi.org/10.3892/or.2013.2235
MLA
Feng, Z., Li, H., Liu, S., Cheng, J., Xiang, G., Zhang, J."FAM172A induces S phase arrest of HepG2 cells via Notch 3 Retraction in /10.3892/or.2023.8554". Oncology Reports 29.3 (2013): 1154-1160.
Chicago
Feng, Z., Li, H., Liu, S., Cheng, J., Xiang, G., Zhang, J."FAM172A induces S phase arrest of HepG2 cells via Notch 3 Retraction in /10.3892/or.2023.8554". Oncology Reports 29, no. 3 (2013): 1154-1160. https://doi.org/10.3892/or.2013.2235
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team