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Article

Efficient downregulation of ErbB-2 induces TACC1 upregulation in breast cancer cell lines

  • Authors:
    • Jinyu Xiang
    • Wensheng Qiu
    • Xingang Wang
    • Fang Zhou
    • Zhen Wang
    • Shihai Liu
    • Lu Yue
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, The Affiliated Hospital of the Medical College, Qingdao University, Qingdao 266003, P.R. China, Department of Galactophore Surgery, The Affiliated Hospital of the Medical College, Qingdao University, Qingdao 266003, P.R. China, Department of Central Laboratory, The Affiliated Hospital of the Medical College, Qingdao University, Qingdao 266003, P.R. China
  • Pages: 1517-1523
    |
    Published online on: January 24, 2013
       https://doi.org/10.3892/or.2013.2253
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Abstract

The ErbB-2 gene, whose overexpression is observed in many types of tumors including breast cancer, plays an important role in carcinoma formation. Dysregulation of the human transforming acidic coiled-coil 1 (TACC1) and ErbB-2 genes is thought to be important in the development and progression of breast cancer. However, a putative interaction between ErbB-2 and TACC1 remains undetermined in breast cancer. After infecting BT474 cells with lentiviral-mediated ErbB2-specific shRNA, we detected the expression of ErbB-2 and TACC1 by real-time PCR and western blotting. ErbB-2 mRNA expression was decreased in the Lenti-ShERBB2 infected cells, and western blotting indicated a concordant reduction in ErbB-2 protein. TACC1 expression at the mRNA and protein levels was significantly upregulated by ErbB-2 silencing in BT474 cells. CCK-8 assay indicated that the inhibition of ErbB-2 expression increased the sensitivity of BT474 cells to docetaxel treatment. These findings provide proof and the foundation for the molecular and biological relationships of ErbB-2 and TACC1 in breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Xiang J, Qiu W, Wang X, Zhou F, Wang Z, Liu S and Yue L: Efficient downregulation of ErbB-2 induces TACC1 upregulation in breast cancer cell lines. Oncol Rep 29: 1517-1523, 2013.
APA
Xiang, J., Qiu, W., Wang, X., Zhou, F., Wang, Z., Liu, S., & Yue, L. (2013). Efficient downregulation of ErbB-2 induces TACC1 upregulation in breast cancer cell lines. Oncology Reports, 29, 1517-1523. https://doi.org/10.3892/or.2013.2253
MLA
Xiang, J., Qiu, W., Wang, X., Zhou, F., Wang, Z., Liu, S., Yue, L."Efficient downregulation of ErbB-2 induces TACC1 upregulation in breast cancer cell lines". Oncology Reports 29.4 (2013): 1517-1523.
Chicago
Xiang, J., Qiu, W., Wang, X., Zhou, F., Wang, Z., Liu, S., Yue, L."Efficient downregulation of ErbB-2 induces TACC1 upregulation in breast cancer cell lines". Oncology Reports 29, no. 4 (2013): 1517-1523. https://doi.org/10.3892/or.2013.2253
Copy and paste a formatted citation
x
Spandidos Publications style
Xiang J, Qiu W, Wang X, Zhou F, Wang Z, Liu S and Yue L: Efficient downregulation of ErbB-2 induces TACC1 upregulation in breast cancer cell lines. Oncol Rep 29: 1517-1523, 2013.
APA
Xiang, J., Qiu, W., Wang, X., Zhou, F., Wang, Z., Liu, S., & Yue, L. (2013). Efficient downregulation of ErbB-2 induces TACC1 upregulation in breast cancer cell lines. Oncology Reports, 29, 1517-1523. https://doi.org/10.3892/or.2013.2253
MLA
Xiang, J., Qiu, W., Wang, X., Zhou, F., Wang, Z., Liu, S., Yue, L."Efficient downregulation of ErbB-2 induces TACC1 upregulation in breast cancer cell lines". Oncology Reports 29.4 (2013): 1517-1523.
Chicago
Xiang, J., Qiu, W., Wang, X., Zhou, F., Wang, Z., Liu, S., Yue, L."Efficient downregulation of ErbB-2 induces TACC1 upregulation in breast cancer cell lines". Oncology Reports 29, no. 4 (2013): 1517-1523. https://doi.org/10.3892/or.2013.2253
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