Efficient downregulation of ErbB-2 induces TACC1 upregulation in breast cancer cell lines

  • Authors:
    • Jinyu Xiang
    • Wensheng Qiu
    • Xingang Wang
    • Fang Zhou
    • Zhen Wang
    • Shihai Liu
    • Lu Yue
  • View Affiliations

  • Published online on: January 24, 2013     https://doi.org/10.3892/or.2013.2253
  • Pages: 1517-1523
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The ErbB-2 gene, whose overexpression is observed in many types of tumors including breast cancer, plays an important role in carcinoma formation. Dysregulation of the human transforming acidic coiled-coil 1 (TACC1) and ErbB-2 genes is thought to be important in the development and progression of breast cancer. However, a putative interaction between ErbB-2 and TACC1 remains undetermined in breast cancer. After infecting BT474 cells with lentiviral-mediated ErbB2-specific shRNA, we detected the expression of ErbB-2 and TACC1 by real-time PCR and western blotting. ErbB-2 mRNA expression was decreased in the Lenti-ShERBB2 infected cells, and western blotting indicated a concordant reduction in ErbB-2 protein. TACC1 expression at the mRNA and protein levels was significantly upregulated by ErbB-2 silencing in BT474 cells. CCK-8 assay indicated that the inhibition of ErbB-2 expression increased the sensitivity of BT474 cells to docetaxel treatment. These findings provide proof and the foundation for the molecular and biological relationships of ErbB-2 and TACC1 in breast cancer.
View Figures
View References

Related Articles

Journal Cover

April 2013
Volume 29 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Xiang J, Qiu W, Wang X, Zhou F, Wang Z, Liu S and Yue L: Efficient downregulation of ErbB-2 induces TACC1 upregulation in breast cancer cell lines. Oncol Rep 29: 1517-1523, 2013
APA
Xiang, J., Qiu, W., Wang, X., Zhou, F., Wang, Z., Liu, S., & Yue, L. (2013). Efficient downregulation of ErbB-2 induces TACC1 upregulation in breast cancer cell lines. Oncology Reports, 29, 1517-1523. https://doi.org/10.3892/or.2013.2253
MLA
Xiang, J., Qiu, W., Wang, X., Zhou, F., Wang, Z., Liu, S., Yue, L."Efficient downregulation of ErbB-2 induces TACC1 upregulation in breast cancer cell lines". Oncology Reports 29.4 (2013): 1517-1523.
Chicago
Xiang, J., Qiu, W., Wang, X., Zhou, F., Wang, Z., Liu, S., Yue, L."Efficient downregulation of ErbB-2 induces TACC1 upregulation in breast cancer cell lines". Oncology Reports 29, no. 4 (2013): 1517-1523. https://doi.org/10.3892/or.2013.2253