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Article

Biological evaluation of 9-[(6-chloropyridin-4-yl)methyl]-9H-carbazole-3-carbinol as an anticancer agent

  • Authors:
    • Chung-Hsien Liu
    • Chingju Lin
    • Kan-Jen Tsai
    • Yi-Ching Chuang
    • Ya-Ling Huang
    • Tsung-Hsien Lee
    • Li-Jiau Huang
    • Hsu-Chin Chan
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, School of Medicine, Chung Shan Medical University and Chung Shan Medical University Hospital, Taichung 40242, Taiwan, R.O.C., Department of Physiology, School of Medicine, China Medical University, Taichung 40402, Taiwan, R.O.C., School of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung 40242, Taiwan, R.O.C., Graduate Institute of Basic Medical Science, China Medical University, Taichung 40402, Taiwan, R.O.C., Graduate Institute of Pharmaceutical Chemistry, China Medical University, Taichung 40402, Taiwan, R.O.C., Department of Obstetrics and Gynecology, School of Medicine, Chung Shan Medical University and Chung Shan Medical University Hospital, Taichung 40242, Taiwan, R.O.C., Department of Biochemistry, School of Medicine, China Medical University, Taichung 40402, Taiwan, R.O.C.
  • Pages: 1501-1509
    |
    Published online on: January 29, 2013
       https://doi.org/10.3892/or.2013.2255
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Abstract

Most conventional anticancer drugs exert either anti-proliferation or anti-angiogenesis activity. Recently, searching for potential multi-target agents has become an alternative strategy for cancer treatment. Several structurally different carbazole alkaloids from either natural or synthesized sources represent an important and heterogeneous class of anticancer agents. In the present study, we investigated the anticancer activity of a novel synthetic carbazole derivative, 9-[(6-chloropyridin-4-yl)methyl]-9H-carbazole-3-carbinol (HYL-6d), which is structurally different from other previously characterized carbazoles. HYL-6d-treated human breast cancer MCF-7 cells exhibited an increased population arrested at the sub-G1 and S phases, as well as an increase of p53 and decrease of cyclin D1, A and CDK2. Also, HYL-6d treatment induced MCF-7 cell apoptosis and this was accompanied by a decreased expression of Bcl-2, increased levels of p53 and Bcl-XS and the activation of caspase-9. Experimental results from human umbilical vascular endothelial cells (HUVECs) showed that HYL-6d also exerted its anti-angiogenic activity in HUVECs by inhibiting cell proliferation, migration, and tube formation induced by VEGF- or bFGF in vitro. In summary, the data indicate that HYL-6d exhibits both cytotoxic effects against human cancer cells and anti-angiogenic activities, which make it a potential therapeutic drug for cancer treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Liu C, Lin C, Tsai K, Chuang Y, Huang Y, Lee T, Huang L and Chan H: Biological evaluation of 9-[(6-chloropyridin-4-yl)methyl]-9H-carbazole-3-carbinol as an anticancer agent. Oncol Rep 29: 1501-1509, 2013.
APA
Liu, C., Lin, C., Tsai, K., Chuang, Y., Huang, Y., Lee, T. ... Chan, H. (2013). Biological evaluation of 9-[(6-chloropyridin-4-yl)methyl]-9H-carbazole-3-carbinol as an anticancer agent. Oncology Reports, 29, 1501-1509. https://doi.org/10.3892/or.2013.2255
MLA
Liu, C., Lin, C., Tsai, K., Chuang, Y., Huang, Y., Lee, T., Huang, L., Chan, H."Biological evaluation of 9-[(6-chloropyridin-4-yl)methyl]-9H-carbazole-3-carbinol as an anticancer agent". Oncology Reports 29.4 (2013): 1501-1509.
Chicago
Liu, C., Lin, C., Tsai, K., Chuang, Y., Huang, Y., Lee, T., Huang, L., Chan, H."Biological evaluation of 9-[(6-chloropyridin-4-yl)methyl]-9H-carbazole-3-carbinol as an anticancer agent". Oncology Reports 29, no. 4 (2013): 1501-1509. https://doi.org/10.3892/or.2013.2255
Copy and paste a formatted citation
x
Spandidos Publications style
Liu C, Lin C, Tsai K, Chuang Y, Huang Y, Lee T, Huang L and Chan H: Biological evaluation of 9-[(6-chloropyridin-4-yl)methyl]-9H-carbazole-3-carbinol as an anticancer agent. Oncol Rep 29: 1501-1509, 2013.
APA
Liu, C., Lin, C., Tsai, K., Chuang, Y., Huang, Y., Lee, T. ... Chan, H. (2013). Biological evaluation of 9-[(6-chloropyridin-4-yl)methyl]-9H-carbazole-3-carbinol as an anticancer agent. Oncology Reports, 29, 1501-1509. https://doi.org/10.3892/or.2013.2255
MLA
Liu, C., Lin, C., Tsai, K., Chuang, Y., Huang, Y., Lee, T., Huang, L., Chan, H."Biological evaluation of 9-[(6-chloropyridin-4-yl)methyl]-9H-carbazole-3-carbinol as an anticancer agent". Oncology Reports 29.4 (2013): 1501-1509.
Chicago
Liu, C., Lin, C., Tsai, K., Chuang, Y., Huang, Y., Lee, T., Huang, L., Chan, H."Biological evaluation of 9-[(6-chloropyridin-4-yl)methyl]-9H-carbazole-3-carbinol as an anticancer agent". Oncology Reports 29, no. 4 (2013): 1501-1509. https://doi.org/10.3892/or.2013.2255
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