Expression profiles of microRNAs and their target genes in papillary thyroid carcinoma

Liu,Xin; He,Mengzi; Hou,Yufei; Liang,Bing; Zhao,Longyu; Ma,Shumei; Yu,Yaqin; Liu,Xiaodong

doi:10.3892/or.2013.2263

Expression profiles of microRNAs and their target genes in papillary thyroid carcinoma

Authors:
- Xin Liu
- Mengzi He
- Yufei Hou
- Bing Liang
- Longyu Zhao
- Shumei Ma
- Yaqin Yu
- Xiaodong Liu
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Affiliations: Department of Bioinformatics and Statistics, Jilin University, Changchun 130021, P.R. China, Key Laboratory of Radiobiology (Ministry of Health), School of Public Health, Jilin University, Changchun 130021, P.R. China, School of Public Health, Jilin University, Changchun 130021, P.R. China, Department of Epidemiology, School of Public Health, Jilin University, Changchun 130021, P.R. China
Published online on: January 31, 2013 https://doi.org/10.3892/or.2013.2263
Pages: 1415-1420

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Abstract

The incidence of thyroid cancer has recently experienced a rapid increase in China, and papillary thyroid carcinoma (PTC) accounts for nearly 80% of human thyroid cancers. In the present study, the differential expression of microRNAs (miRNAs) and their target genes were identified in order to analyze the potential roles of miRNAs as biomarkers and in papillary thyroid carcinogenesis. One hundred and twenty-six PTC samples were collected from patients at the China-Japan Union Hospital, China, and the gene/miRNA expression profiles were examined with Illumina BeadChips and verified by real‑time RT-PCR. Gene Ontology (GO) categories were determined, and pathway analysis was carried out using KEGG. miRNA target genes were predicted by implementing three computational analysis programs: TargetScanS, DIANA-microT and PicTar. Two hundred and forty-eight miRNAs and 3,631 genes were found to be significantly deregulated (gene, P<0.05; miRNA, P<0.01) in PTC tissues when compared with their matching normal thyroid tissues. hsa-miR-206 (target gene, MET), hsa-miR-299-3p (target gene, ITGAV), hsa-miR-101 (target gene, ITGA3), hsa-miR-103 (target gene, ITGA2), hsa-miR‑222 (target genes, KIT and AXIN2), hsa-miR-15a (target genes, AXIN2 and FOXO1) and hsa-mir-221 (target gene, KIT) were identified. Together with the functions of the target genes, we further elucidated the role of miRNAs in papillary thyroid carcinogenesis and suggest the use of miRNAs as biomarkers for early diagnosis. Our findings provide the basis for future studies in the field of miRNA-based cancer therapy.

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