The saponin monomer of dwarf lilyturf tuber, DT-13, inhibits angiogenesis under hypoxia and normoxia via multi-targeting activity

  • Authors:
    • Renping Zhao
    • Li Sun
    • Sensen Lin
    • Xianshu Bai
    • Boyang Yu
    • Shengtao Yuan
    • Luyong Zhang
  • View Affiliations

  • Published online on: February 5, 2013     https://doi.org/10.3892/or.2013.2272
  • Pages: 1379-1386
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Abstract

The saponin monomer of dwarf lilyturf tuber, DT-13, exhibits anticancer activity by reducing human breast cancer cell adhesion and migration under hypoxia. To further investigate the anticancer activity of DT-13, we investigated whether DT-13 exhibits anti-angiogenic activity. DT-13 showed no effect on human umbilical vein endothelial cell proliferation but inhibited tube formation and migration under normoxia and hypoxia. Moreover, DT-13 significantly reduced density of vessels in vivo observed from a chicken chorioallantoic membrane model. Western blotting results showed that DT-13 suppressed the increased level of hypoxia-inducible factor 1α, p-extracellular signal-regulated kinase 1/2 and p-Akt induced by hypoxia. Enzyme-linked immunosorbent assay revealed that vascular endothelial growth factor excretion was suppressed by DT-13. DT-13 inhibited migration and tube formation induced by vascular endothelial growth factor under normoxia and hypoxia. In addition, DT-13 reduced the level of p-vascular endothelial growth factor receptor 2 and p-Akt induced by vascular endothelial growth factor. Our data suggest that DT-13 inhibits angiogenesis under normoxia and hypoxia and also inhibits angiogenesis induced by vascular endothelial growth factor via targeting at multi elements.
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April 2013
Volume 29 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhao R, Sun L, Lin S, Bai X, Yu B, Yuan S and Zhang L: The saponin monomer of dwarf lilyturf tuber, DT-13, inhibits angiogenesis under hypoxia and normoxia via multi-targeting activity. Oncol Rep 29: 1379-1386, 2013
APA
Zhao, R., Sun, L., Lin, S., Bai, X., Yu, B., Yuan, S., & Zhang, L. (2013). The saponin monomer of dwarf lilyturf tuber, DT-13, inhibits angiogenesis under hypoxia and normoxia via multi-targeting activity. Oncology Reports, 29, 1379-1386. https://doi.org/10.3892/or.2013.2272
MLA
Zhao, R., Sun, L., Lin, S., Bai, X., Yu, B., Yuan, S., Zhang, L."The saponin monomer of dwarf lilyturf tuber, DT-13, inhibits angiogenesis under hypoxia and normoxia via multi-targeting activity". Oncology Reports 29.4 (2013): 1379-1386.
Chicago
Zhao, R., Sun, L., Lin, S., Bai, X., Yu, B., Yuan, S., Zhang, L."The saponin monomer of dwarf lilyturf tuber, DT-13, inhibits angiogenesis under hypoxia and normoxia via multi-targeting activity". Oncology Reports 29, no. 4 (2013): 1379-1386. https://doi.org/10.3892/or.2013.2272