Active immunotherapy for mouse breast cancer with irradiated whole-cell vaccine expressing VEGFR2

  • Authors:
    • Heng-Xiu Yan
    • Ping Cheng
    • Hai-Yan Wei
    • Guo-Bo Shen
    • Li-Xin Fu
    • Jie Ni
    • Yang Wu
    • Yu-Quan Wei
  • View Affiliations

  • Published online on: February 7, 2013     https://doi.org/10.3892/or.2013.2282
  • Pages: 1510-1516
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Abstract

As tumor-associated antigens are not well characterized for the majority of human tumors, polyvalent vaccines prepared with whole-tumor antigens are an attractive approach for tumor vaccination. Vascular endothelial growth factor receptor-2 (VEGFR2), as a model antigen with which to explore the feasibility of immunotherapy, has shown great promise as a tumor vaccine. However, the efficacy of immunotherapy is often not ideal when used alone. In this study, we explored the therapeutic efficacy of an irradiated AdVEGFR2-infected cell vaccine-based immunotherapy in the weakly immunogenic and highly metastatic 4T1 murine mammary cancer model. An adenovirus encoding the VEGFR2 gene (AdVEGFR2) was constructed. Lethally irradiated, virus-infected 4T1 cells were used as vaccines. Vaccination with lethally irradiated AdVEGFR2-infected 4T1 cells inhibited subsequent tumor growth and pulmonary metastasis compared with challenge inoculations. Angiogenesis was inhibited, and the number of CD8+ T lymphocytes was increased within the tumors. Antitumor activity was also caused by the adoptive transfer of isolated spleen lymphocytes. In vitro, the expression of HMGB1 and HSP70 in the AdVEGFR2‑infected 4T1 cells was increased, and was involved in the activation of tumor antigen-specific T-cell immunity. Our results indicate that the immunotherapy based on irradiated AdVEGFR2-infected whole-cancer cell vaccines may be a potentially effective strategy for 4T1 cancer treatment.
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April 2013
Volume 29 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yan H, Cheng P, Wei H, Shen G, Fu L, Ni J, Wu Y and Wei Y: Active immunotherapy for mouse breast cancer with irradiated whole-cell vaccine expressing VEGFR2. Oncol Rep 29: 1510-1516, 2013.
APA
Yan, H., Cheng, P., Wei, H., Shen, G., Fu, L., Ni, J. ... Wei, Y. (2013). Active immunotherapy for mouse breast cancer with irradiated whole-cell vaccine expressing VEGFR2. Oncology Reports, 29, 1510-1516. https://doi.org/10.3892/or.2013.2282
MLA
Yan, H., Cheng, P., Wei, H., Shen, G., Fu, L., Ni, J., Wu, Y., Wei, Y."Active immunotherapy for mouse breast cancer with irradiated whole-cell vaccine expressing VEGFR2". Oncology Reports 29.4 (2013): 1510-1516.
Chicago
Yan, H., Cheng, P., Wei, H., Shen, G., Fu, L., Ni, J., Wu, Y., Wei, Y."Active immunotherapy for mouse breast cancer with irradiated whole-cell vaccine expressing VEGFR2". Oncology Reports 29, no. 4 (2013): 1510-1516. https://doi.org/10.3892/or.2013.2282