Poly(amido amine) is an ideal carrier of miR-7 for enhancing gene silencing effects on the EGFR pathway in U251 glioma cells

Liu,Xiaozhi; Li,Gang; Su,Zhiguo; Jiang,Zhongmin; Chen,Lei; Wang,Junfei; Yu,Shizhu; Liu,Zhenlin

doi:10.3892/or.2013.2283

April 2013 Volume 29 Issue 4

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April 2013 Volume 29 Issue 4

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Article

Poly(amido amine) is an ideal carrier of miR-7 for enhancing gene silencing effects on the EGFR pathway in U251 glioma cells

Authors:
- Xiaozhi Liu
- Gang Li
- Zhiguo Su
- Zhongmin Jiang
- Lei Chen
- Junfei Wang
- Shizhu Yu
- Zhenlin Liu
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Affiliations: Department of Neurosurgery, The Fifth Central Hospital of Tianjin, Tianjin, P.R. China, Department of Pathology, The Fifth Central Hospital of Tianjin, Tianjin, P.R. China, Department of Neuropathology Institute, Department of Tianjin Neurological, Tianjin Medical University General Hospital, Tianjin, P.R. China
Pages: 1387-1394
|
Published online on: February 11, 2013

https://doi.org/10.3892/or.2013.2283
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Abstract

microRNAs are regarded as promising drugs for glioma gene therapy. However, conventional administration routes, such as oral administration and intravenous infusion, present low efficiency due to the blood-brain barrier and intercellular retention, thereby limiting their application. Recent studies showed poly(amido amine) (PAMAM) was a candidate carrier due to its high solubilization, delayed release and low toxicity. In the present study, U251 human brain glioma cells were transfected with the miR-7 gene using PAMAM as the vector to determine the transfection efficiency and therapeutic effects in vivo and in vitro. We found that PAMAM exhibited higher transfection efficiency and longer duration of action compared with liposome delivery, and miR-7 efficiently silenced some genes involved in the epidermal growth factor receptor (EGFR) pathway and achieved favorable effects in treating glioma in vivo and in vitro. These investigations provide a basis for developing high-efficiency micromolecular drug delivery.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Poly(amido amine) is an ideal carrier of miR-7 for enhancing gene silencing effects on the EGFR pathway in U251 glioma cells

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