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Article

Downregulation of microRNA-138 enhances the proliferation, migration and invasion of cholangiocarcinoma cells through the upregulation of RhoC/p-ERK/MMP-2/MMP-9

  • Authors:
    • Qi Wang
    • Huihuan Tang
    • Shanshan Yin
    • Chao Dong
  • View Affiliations / Copyright

    Affiliations: Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
  • Pages: 2046-2052
    |
    Published online on: February 26, 2013
       https://doi.org/10.3892/or.2013.2304
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Abstract

microRNAs (miRs) play an important role in tumor initiation and progression in many types of cancer, including cholangiocarcinoma (CC). miR-138 dysregulation is frequently observed in a variety of tumors. In the present study, miR-138 was found to be downregulated in CC tissues by quantitative real-time RT-PCR. Furthermore, its potential target molecule, Ras homolog gene family, member C (RhoC) protein, was found to be highly expressed in CC tissues examined by western blot analysis. Luciferase reporter assay further demonstrated that miR-138 directly targeted RhoC. We found that the introduction of miR-138 mimics to RBE and QBC939 CC cells could reduced RhoC mRNA and protein expression, and suppressed the proliferation, G1/S transition, migration and invasion of CC cells. However, transfection with a miR-138 inhibitor induced an inverse effect in CC cells. The expression of phosphorylated extracellular signal-regulated kinase (p-ERK), matrix metalloproteinase (MMP)-2 and MMP-9 decreased following transfection with miR-138, and increased following transfection with miR-138 inhibitor in CC cells. In conclusion, RhoC upregulation induced by miR-138 downregulation promotes the malignant progression of CC cells and the underlying mechanisms of this effect involve the increase in the expression of p-ERK/MMP-2/MMP-9. Consequently, miR-138/RhoC is a potential target for the clinical diagnosis and treatment of CC.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Q, Tang H, Yin S and Dong C: Downregulation of microRNA-138 enhances the proliferation, migration and invasion of cholangiocarcinoma cells through the upregulation of RhoC/p-ERK/MMP-2/MMP-9. Oncol Rep 29: 2046-2052, 2013.
APA
Wang, Q., Tang, H., Yin, S., & Dong, C. (2013). Downregulation of microRNA-138 enhances the proliferation, migration and invasion of cholangiocarcinoma cells through the upregulation of RhoC/p-ERK/MMP-2/MMP-9. Oncology Reports, 29, 2046-2052. https://doi.org/10.3892/or.2013.2304
MLA
Wang, Q., Tang, H., Yin, S., Dong, C."Downregulation of microRNA-138 enhances the proliferation, migration and invasion of cholangiocarcinoma cells through the upregulation of RhoC/p-ERK/MMP-2/MMP-9". Oncology Reports 29.5 (2013): 2046-2052.
Chicago
Wang, Q., Tang, H., Yin, S., Dong, C."Downregulation of microRNA-138 enhances the proliferation, migration and invasion of cholangiocarcinoma cells through the upregulation of RhoC/p-ERK/MMP-2/MMP-9". Oncology Reports 29, no. 5 (2013): 2046-2052. https://doi.org/10.3892/or.2013.2304
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Q, Tang H, Yin S and Dong C: Downregulation of microRNA-138 enhances the proliferation, migration and invasion of cholangiocarcinoma cells through the upregulation of RhoC/p-ERK/MMP-2/MMP-9. Oncol Rep 29: 2046-2052, 2013.
APA
Wang, Q., Tang, H., Yin, S., & Dong, C. (2013). Downregulation of microRNA-138 enhances the proliferation, migration and invasion of cholangiocarcinoma cells through the upregulation of RhoC/p-ERK/MMP-2/MMP-9. Oncology Reports, 29, 2046-2052. https://doi.org/10.3892/or.2013.2304
MLA
Wang, Q., Tang, H., Yin, S., Dong, C."Downregulation of microRNA-138 enhances the proliferation, migration and invasion of cholangiocarcinoma cells through the upregulation of RhoC/p-ERK/MMP-2/MMP-9". Oncology Reports 29.5 (2013): 2046-2052.
Chicago
Wang, Q., Tang, H., Yin, S., Dong, C."Downregulation of microRNA-138 enhances the proliferation, migration and invasion of cholangiocarcinoma cells through the upregulation of RhoC/p-ERK/MMP-2/MMP-9". Oncology Reports 29, no. 5 (2013): 2046-2052. https://doi.org/10.3892/or.2013.2304
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