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Article

Sorting, identification and enrichment of side population cells in THP-1 acute monocytic leukemia cells

  • Authors:
    • Yingchao Wang
    • Chuyun Yin
    • Lei Feng
    • Lina Ma
    • Yongwei Wei
    • Guangyao Sheng
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
  • Pages: 1923-1931
    |
    Published online on: February 28, 2013
       https://doi.org/10.3892/or.2013.2316
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Abstract

The objective of the present study was to examine and determine whether the human acute monocytic leukemia cell line THP-1 contains side population (SP) cells, and, if so, to increase the proportion of SP cells using arabinosylcytosine (Ara-C). Fluorescent microscopy and flow cytometry were employed to detect the percentage of SP cells in THP-1 cells. Then, SP and non-SP (NSP) cell subpopulations were collected and identified. THP-1 cells were incubated with different concentrations of Ara-C for 24 h and the proportion of SP cells was detected. Our results demonstrated that the percentage of SP cells was 1.81±0.99% in THP-1 cells. A majority of the SP cells remained in the G0/G1 phase, and the expression of CD34+ and CD34+CD38- and the proliferation ability of the SP cells were higher compared to NSP cells (P<0.05). The mRNA expression of multidrug resistance genes (ABCG2 and ABCB1), apoptosis regulation genes (Bcl-2) and the Bcl-2/Bax value of SP cells were higher than those of NSP cells. SP cells have been shown to be more tumorigenic than NSP cells. Following co-culture with Ara-C, the proportion of SP cells increased significantly and subsequently the Ara-C concentration increased. These findings suggest that the THP-1 cell line contains SP cells and that SP cells possess certain intrinsic stem cell properties and may contain a larger proportion of leukemia stem cells (LSCs). The concentrations of SP cells can be increased with Ara-C by co-culture, and this technique is a useful and important application for the study of LSCs.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Yin C, Feng L, Ma L, Wei Y and Sheng G: Sorting, identification and enrichment of side population cells in THP-1 acute monocytic leukemia cells. Oncol Rep 29: 1923-1931, 2013.
APA
Wang, Y., Yin, C., Feng, L., Ma, L., Wei, Y., & Sheng, G. (2013). Sorting, identification and enrichment of side population cells in THP-1 acute monocytic leukemia cells. Oncology Reports, 29, 1923-1931. https://doi.org/10.3892/or.2013.2316
MLA
Wang, Y., Yin, C., Feng, L., Ma, L., Wei, Y., Sheng, G."Sorting, identification and enrichment of side population cells in THP-1 acute monocytic leukemia cells". Oncology Reports 29.5 (2013): 1923-1931.
Chicago
Wang, Y., Yin, C., Feng, L., Ma, L., Wei, Y., Sheng, G."Sorting, identification and enrichment of side population cells in THP-1 acute monocytic leukemia cells". Oncology Reports 29, no. 5 (2013): 1923-1931. https://doi.org/10.3892/or.2013.2316
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Yin C, Feng L, Ma L, Wei Y and Sheng G: Sorting, identification and enrichment of side population cells in THP-1 acute monocytic leukemia cells. Oncol Rep 29: 1923-1931, 2013.
APA
Wang, Y., Yin, C., Feng, L., Ma, L., Wei, Y., & Sheng, G. (2013). Sorting, identification and enrichment of side population cells in THP-1 acute monocytic leukemia cells. Oncology Reports, 29, 1923-1931. https://doi.org/10.3892/or.2013.2316
MLA
Wang, Y., Yin, C., Feng, L., Ma, L., Wei, Y., Sheng, G."Sorting, identification and enrichment of side population cells in THP-1 acute monocytic leukemia cells". Oncology Reports 29.5 (2013): 1923-1931.
Chicago
Wang, Y., Yin, C., Feng, L., Ma, L., Wei, Y., Sheng, G."Sorting, identification and enrichment of side population cells in THP-1 acute monocytic leukemia cells". Oncology Reports 29, no. 5 (2013): 1923-1931. https://doi.org/10.3892/or.2013.2316
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