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Article Open Access

PARP1 gene expression is downregulated by knockdown of PARG gene

  • Authors:
    • Fumiaki Uchiumi
    • Takeshi Watanabe
    • Ryo Ohta
    • Hideaki Abe
    • Sei-Ichi Tanuma
  • View Affiliations / Copyright

    Affiliations: Department of Gene Regulation, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda-shi, Chiba-ken 278-8510, Japan, Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda-shi, Chiba-ken 278-8510, Japan, Department of Biochemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Noda-shi, Chiba-ken 278-8510, Japan
    Copyright: © Uchiumi et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1683-1688
    |
    Published online on: March 4, 2013
       https://doi.org/10.3892/or.2013.2321
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Abstract

Poly(ADP-ribosyl)ation is a modification of nuclear proteins that regulates DNA replication, repair and transcription. In order to investigate the biological effects of degradation of poly(ADP-ribose), knockdown of the poly(ADP-ribose) glycohydrolase (PARG) gene was performed by introducing a short interfering RNA (siRNA)-pool into HeLa S3 cells. Notably, poly(ADP-ribosyl)ated proteins did not accumulate in the cells. Western blotting, quantitative RT-PCR analysis and a transient transfection assay revealed that poly(ADP-ribose) polymerase 1 (PARP1) gene/protein expression and its promoter activity were reduced in the PARG knockdown cells. These results suggest that the amount of poly(ADP-ribose) in a cell is regulated under the control of PARP1/PARG gene expression balance. Furthermore, in this study, we showed that PARG-siRNA enhanced cell death induced by staurosporine (STS). Thus, we propose a PARG-siRNA utilizing gene-therapy for cancer treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Uchiumi F, Watanabe T, Ohta R, Abe H and Tanuma S: PARP1 gene expression is downregulated by knockdown of PARG gene. Oncol Rep 29: 1683-1688, 2013.
APA
Uchiumi, F., Watanabe, T., Ohta, R., Abe, H., & Tanuma, S. (2013). PARP1 gene expression is downregulated by knockdown of PARG gene. Oncology Reports, 29, 1683-1688. https://doi.org/10.3892/or.2013.2321
MLA
Uchiumi, F., Watanabe, T., Ohta, R., Abe, H., Tanuma, S."PARP1 gene expression is downregulated by knockdown of PARG gene". Oncology Reports 29.5 (2013): 1683-1688.
Chicago
Uchiumi, F., Watanabe, T., Ohta, R., Abe, H., Tanuma, S."PARP1 gene expression is downregulated by knockdown of PARG gene". Oncology Reports 29, no. 5 (2013): 1683-1688. https://doi.org/10.3892/or.2013.2321
Copy and paste a formatted citation
x
Spandidos Publications style
Uchiumi F, Watanabe T, Ohta R, Abe H and Tanuma S: PARP1 gene expression is downregulated by knockdown of PARG gene. Oncol Rep 29: 1683-1688, 2013.
APA
Uchiumi, F., Watanabe, T., Ohta, R., Abe, H., & Tanuma, S. (2013). PARP1 gene expression is downregulated by knockdown of PARG gene. Oncology Reports, 29, 1683-1688. https://doi.org/10.3892/or.2013.2321
MLA
Uchiumi, F., Watanabe, T., Ohta, R., Abe, H., Tanuma, S."PARP1 gene expression is downregulated by knockdown of PARG gene". Oncology Reports 29.5 (2013): 1683-1688.
Chicago
Uchiumi, F., Watanabe, T., Ohta, R., Abe, H., Tanuma, S."PARP1 gene expression is downregulated by knockdown of PARG gene". Oncology Reports 29, no. 5 (2013): 1683-1688. https://doi.org/10.3892/or.2013.2321
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