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Sequence mutations of the substrate binding pocket of stem cell factor and multidrug resistance protein ABCG2 in renal cell cancer: A possible link to treatment resistance

  • Authors:
    • Inka Zoernig
    • Claudia Ziegelmeier
    • Bernd Lahrmann
    • Niels  Grabe
    • Dirk Jäger
    • Niels Halama
  • View Affiliations / Copyright

    Affiliations: Department of Medical Oncology, National Center for Tumor Diseases, University of Heidelberg, D-69120 Heidelberg, Germany, Tissue Imaging and Analysis Center (TIGA) and National Center for Tumor Diseases, D-69120 Heidelberg, Germany
    Copyright: © Zoernig et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1697-1700
    |
    Published online on: March 5, 2013
       https://doi.org/10.3892/or.2013.2324
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Abstract

ABCG2 is a multidrug cellular transport protein that is associated with resistance to certain treatments in patients, particularly anticancer treatment. The tumor-protective properties of ABCG2 expression are reported to be a feature of a subset of stem cell-like tumor cells. While protection against chemotherapy has been well analyzed, the role of ABCG2 in the treatment with tyrosine kinase inhibitors is only partially understood. Tyrosine kinase inhibitors are currently the main treatment option in irresectable renal cell carcinomas. To investigate possible underlying sequence variations in the ABCG2 gene with relevance to the functional properties of the protein, 36 patient samples were analyzed. Using sequence analysis and single-nucleotide polymorphism databases, sequence variations in the highly conserved domains of the binding pocket of ABCG2 were analyzed. The resulting variations were used for computational protein prediction algorithms to identify conformational alterations. A relevant shift from A to G at position 1376 (resulting in Y→C at 459 aa) was identified and found to be present in 8.3% of the patients. These patients are currently in follow-up after resection, thus, further analysis will reveal whether this mutation has relevance to treatment efficacy.
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Copy and paste a formatted citation
Spandidos Publications style
Zoernig I, Ziegelmeier C, Lahrmann B, Grabe N, Jäger D and Halama N: Sequence mutations of the substrate binding pocket of stem cell factor and multidrug resistance protein ABCG2 in renal cell cancer: A possible link to treatment resistance. Oncol Rep 29: 1697-1700, 2013.
APA
Zoernig, I., Ziegelmeier, C., Lahrmann, B., Grabe, N., Jäger, D., & Halama, N. (2013). Sequence mutations of the substrate binding pocket of stem cell factor and multidrug resistance protein ABCG2 in renal cell cancer: A possible link to treatment resistance. Oncology Reports, 29, 1697-1700. https://doi.org/10.3892/or.2013.2324
MLA
Zoernig, I., Ziegelmeier, C., Lahrmann, B., Grabe, N., Jäger, D., Halama, N."Sequence mutations of the substrate binding pocket of stem cell factor and multidrug resistance protein ABCG2 in renal cell cancer: A possible link to treatment resistance". Oncology Reports 29.5 (2013): 1697-1700.
Chicago
Zoernig, I., Ziegelmeier, C., Lahrmann, B., Grabe, N., Jäger, D., Halama, N."Sequence mutations of the substrate binding pocket of stem cell factor and multidrug resistance protein ABCG2 in renal cell cancer: A possible link to treatment resistance". Oncology Reports 29, no. 5 (2013): 1697-1700. https://doi.org/10.3892/or.2013.2324
Copy and paste a formatted citation
x
Spandidos Publications style
Zoernig I, Ziegelmeier C, Lahrmann B, Grabe N, Jäger D and Halama N: Sequence mutations of the substrate binding pocket of stem cell factor and multidrug resistance protein ABCG2 in renal cell cancer: A possible link to treatment resistance. Oncol Rep 29: 1697-1700, 2013.
APA
Zoernig, I., Ziegelmeier, C., Lahrmann, B., Grabe, N., Jäger, D., & Halama, N. (2013). Sequence mutations of the substrate binding pocket of stem cell factor and multidrug resistance protein ABCG2 in renal cell cancer: A possible link to treatment resistance. Oncology Reports, 29, 1697-1700. https://doi.org/10.3892/or.2013.2324
MLA
Zoernig, I., Ziegelmeier, C., Lahrmann, B., Grabe, N., Jäger, D., Halama, N."Sequence mutations of the substrate binding pocket of stem cell factor and multidrug resistance protein ABCG2 in renal cell cancer: A possible link to treatment resistance". Oncology Reports 29.5 (2013): 1697-1700.
Chicago
Zoernig, I., Ziegelmeier, C., Lahrmann, B., Grabe, N., Jäger, D., Halama, N."Sequence mutations of the substrate binding pocket of stem cell factor and multidrug resistance protein ABCG2 in renal cell cancer: A possible link to treatment resistance". Oncology Reports 29, no. 5 (2013): 1697-1700. https://doi.org/10.3892/or.2013.2324
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