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Article

Let-7: A regulator of the ERα signaling pathway in human breast tumors and breast cancer stem cells

  • Authors:
    • Xin Sun
    • Sida Qin
    • Chong Fan
    • Chongwen Xu
    • Ning Du
    • Hong Ren
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, The First Affiliated Hospital of Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi 710061, P.R. China
  • Pages: 2079-2087
    |
    Published online on: March 6, 2013
       https://doi.org/10.3892/or.2013.2330
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Abstract

The oncogenic role of estrogen receptor (ER)α and its correlation with let-7 microRNAs (miRNAs) have been studied and confirmed in breast tumors; however, this correlation has not been investigated in breast cancer stem cells (BCSCs). In the present study, we detected the expression of let-7 and ERα in ER-positive breast tumor tissues. Furthermore, we used a FACSAria cell sorter to separate side population (SP) cells from the MCF-7 and T47-D cell lines by Hoechst 33342 staining. The expression of let-7 miRNAs, ERα and its downstream genes in SP and non-SP (NSP) cells were analyzed. In additional experiments, we transfected a plasmid expressing let-7a into SP cells isolated from the MCF-7 and T47-D cell lines in order to observe changes in the expression of downstream genes (cyclin D1 and pS2). The correlation among let-7, ERα and ERα downstream genes suggested that let-7 acts as a tumor suppressor by inhibiting ERα-mediated cellular malignant growth in ER-positive breast cancer stem cells. The suppression of ERα by the upregulation of let-7 expression may be a promising strategy for the inhibition of the ER signaling pathway and for the elimination of cancer stem cells, thus aiding in the treatment of breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Sun X, Qin S, Fan C, Xu C, Du N and Ren H: Let-7: A regulator of the ERα signaling pathway in human breast tumors and breast cancer stem cells. Oncol Rep 29: 2079-2087, 2013.
APA
Sun, X., Qin, S., Fan, C., Xu, C., Du, N., & Ren, H. (2013). Let-7: A regulator of the ERα signaling pathway in human breast tumors and breast cancer stem cells. Oncology Reports, 29, 2079-2087. https://doi.org/10.3892/or.2013.2330
MLA
Sun, X., Qin, S., Fan, C., Xu, C., Du, N., Ren, H."Let-7: A regulator of the ERα signaling pathway in human breast tumors and breast cancer stem cells". Oncology Reports 29.5 (2013): 2079-2087.
Chicago
Sun, X., Qin, S., Fan, C., Xu, C., Du, N., Ren, H."Let-7: A regulator of the ERα signaling pathway in human breast tumors and breast cancer stem cells". Oncology Reports 29, no. 5 (2013): 2079-2087. https://doi.org/10.3892/or.2013.2330
Copy and paste a formatted citation
x
Spandidos Publications style
Sun X, Qin S, Fan C, Xu C, Du N and Ren H: Let-7: A regulator of the ERα signaling pathway in human breast tumors and breast cancer stem cells. Oncol Rep 29: 2079-2087, 2013.
APA
Sun, X., Qin, S., Fan, C., Xu, C., Du, N., & Ren, H. (2013). Let-7: A regulator of the ERα signaling pathway in human breast tumors and breast cancer stem cells. Oncology Reports, 29, 2079-2087. https://doi.org/10.3892/or.2013.2330
MLA
Sun, X., Qin, S., Fan, C., Xu, C., Du, N., Ren, H."Let-7: A regulator of the ERα signaling pathway in human breast tumors and breast cancer stem cells". Oncology Reports 29.5 (2013): 2079-2087.
Chicago
Sun, X., Qin, S., Fan, C., Xu, C., Du, N., Ren, H."Let-7: A regulator of the ERα signaling pathway in human breast tumors and breast cancer stem cells". Oncology Reports 29, no. 5 (2013): 2079-2087. https://doi.org/10.3892/or.2013.2330
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