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Article

Downregulation of OCT4 promotes differentiation and inhibits growth of BE (2)-C human neuroblastoma I-type cells

  • Authors:
    • Lin Yang
    • Jicui Zheng
    • Ting Xu
    • Xianmin Xiao
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, Children's Hospital of Fudan University, Shanghai 201102, P.R. China
  • Pages: 2191-2196
    |
    Published online on: March 21, 2013
       https://doi.org/10.3892/or.2013.2356
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Abstract

In in vitro continuous culture, N (neuroblastic)-, S (substrate adherent)- and I (intermediate)-type cells were identified in human neuroblastoma (NB), where I-type is recognized as a stem cell type. Octamer-binding protein 4 (OCT4) is a cell marker used to identify the stemness of cells, whose roles in regulating I-type NB cells have yet to be fully elucidated. In the present study, we assessed the differentiation regulation role of OCT4 in BE (2)-C of I-type cells. We demonstrated that downregulation of OCT4 expression in BE (2)-C was associated with reduced cell proliferation and loss of colony formation ability on soft agar, and prompted BE (2)-C cells to differentiate to S-type cells. By contrast, overexpression of OCT4 increased cell proliferation and colony formation ratio, but no obvious differentiation promotion was observed. Furthermore, induced differentiation of BE (2)-C cells to S-type by 5-bromo-2'-deoxyuridine (BrdUrd) was accompanied by reduced expression of OCT4.
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Copy and paste a formatted citation
Spandidos Publications style
Yang L, Zheng J, Xu T and Xiao X: Downregulation of OCT4 promotes differentiation and inhibits growth of BE (2)-C human neuroblastoma I-type cells. Oncol Rep 29: 2191-2196, 2013.
APA
Yang, L., Zheng, J., Xu, T., & Xiao, X. (2013). Downregulation of OCT4 promotes differentiation and inhibits growth of BE (2)-C human neuroblastoma I-type cells. Oncology Reports, 29, 2191-2196. https://doi.org/10.3892/or.2013.2356
MLA
Yang, L., Zheng, J., Xu, T., Xiao, X."Downregulation of OCT4 promotes differentiation and inhibits growth of BE (2)-C human neuroblastoma I-type cells". Oncology Reports 29.6 (2013): 2191-2196.
Chicago
Yang, L., Zheng, J., Xu, T., Xiao, X."Downregulation of OCT4 promotes differentiation and inhibits growth of BE (2)-C human neuroblastoma I-type cells". Oncology Reports 29, no. 6 (2013): 2191-2196. https://doi.org/10.3892/or.2013.2356
Copy and paste a formatted citation
x
Spandidos Publications style
Yang L, Zheng J, Xu T and Xiao X: Downregulation of OCT4 promotes differentiation and inhibits growth of BE (2)-C human neuroblastoma I-type cells. Oncol Rep 29: 2191-2196, 2013.
APA
Yang, L., Zheng, J., Xu, T., & Xiao, X. (2013). Downregulation of OCT4 promotes differentiation and inhibits growth of BE (2)-C human neuroblastoma I-type cells. Oncology Reports, 29, 2191-2196. https://doi.org/10.3892/or.2013.2356
MLA
Yang, L., Zheng, J., Xu, T., Xiao, X."Downregulation of OCT4 promotes differentiation and inhibits growth of BE (2)-C human neuroblastoma I-type cells". Oncology Reports 29.6 (2013): 2191-2196.
Chicago
Yang, L., Zheng, J., Xu, T., Xiao, X."Downregulation of OCT4 promotes differentiation and inhibits growth of BE (2)-C human neuroblastoma I-type cells". Oncology Reports 29, no. 6 (2013): 2191-2196. https://doi.org/10.3892/or.2013.2356
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