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Article

Angiotensin-converting enzyme 2 attenuates the metastasis of non-small cell lung cancer through inhibition of epithelial-mesenchymal transition

  • Authors:
    • Yan-Rong Qian
    • Yi Guo
    • Huan-Ying Wan
    • Liang Fan
    • Yun Feng
    • Lei Ni
    • Yi Xiang
    • Qing-Yun Li
  • View Affiliations / Copyright

    Affiliations: Respiratory Department, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, P.R. China
  • Pages: 2408-2414
    |
    Published online on: March 29, 2013
       https://doi.org/10.3892/or.2013.2370
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Abstract

Angiotensin-converting enzyme 2 (ACE2) is a key enzyme of the renin-angiotensin system (RAS). ACE2 plays a critical counterbalancing role by degrading angiotensin II (Ang II) to Ang 1-7. Recent studies suggest that RAS influences tumor growth and development by its paracrine effects on the tumor microenvironment. Epithelial‑mesenchymal transition (EMT) is now thought to be a process that plays a fundamental role in tumor progression and metastasis. In the present study, we investigated the role of ACE2 in lung cancer metastasis and the mechanism of EMT. This is the first study to elucidate the mechanism through which the overexpression of ACE2 in the A549 lung cancer cell line decreases metastasis formation in vivo and upregulates the expression of E-cadherin both in vitro and in vivo. We also observed the downregulation of vimentin, which supports a role of ACE2 in influencing EMT in lung cancer. Further analysis indicated that ACE2 abrogated the upregulation of TGF-β1-induced EMT markers, such as vimentin and α-smooth muscle actin (αSMA) in vitro in A549 cells. Finally, exposing A549 cells stably expressing ACE2 to DX600, an inhibitor of ACE2, recovered the sensitivity of lung cancer cells to TGF-β1-mediated induction of EMT. Our study demonstrated that ACE2 attenuated the metastasis of lung cancer and may serve as a target for new strategies to inhibit EMT in cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Qian Y, Guo Y, Wan H, Fan L, Feng Y, Ni L, Xiang Y and Li Q: Angiotensin-converting enzyme 2 attenuates the metastasis of non-small cell lung cancer through inhibition of epithelial-mesenchymal transition. Oncol Rep 29: 2408-2414, 2013.
APA
Qian, Y., Guo, Y., Wan, H., Fan, L., Feng, Y., Ni, L. ... Li, Q. (2013). Angiotensin-converting enzyme 2 attenuates the metastasis of non-small cell lung cancer through inhibition of epithelial-mesenchymal transition. Oncology Reports, 29, 2408-2414. https://doi.org/10.3892/or.2013.2370
MLA
Qian, Y., Guo, Y., Wan, H., Fan, L., Feng, Y., Ni, L., Xiang, Y., Li, Q."Angiotensin-converting enzyme 2 attenuates the metastasis of non-small cell lung cancer through inhibition of epithelial-mesenchymal transition". Oncology Reports 29.6 (2013): 2408-2414.
Chicago
Qian, Y., Guo, Y., Wan, H., Fan, L., Feng, Y., Ni, L., Xiang, Y., Li, Q."Angiotensin-converting enzyme 2 attenuates the metastasis of non-small cell lung cancer through inhibition of epithelial-mesenchymal transition". Oncology Reports 29, no. 6 (2013): 2408-2414. https://doi.org/10.3892/or.2013.2370
Copy and paste a formatted citation
x
Spandidos Publications style
Qian Y, Guo Y, Wan H, Fan L, Feng Y, Ni L, Xiang Y and Li Q: Angiotensin-converting enzyme 2 attenuates the metastasis of non-small cell lung cancer through inhibition of epithelial-mesenchymal transition. Oncol Rep 29: 2408-2414, 2013.
APA
Qian, Y., Guo, Y., Wan, H., Fan, L., Feng, Y., Ni, L. ... Li, Q. (2013). Angiotensin-converting enzyme 2 attenuates the metastasis of non-small cell lung cancer through inhibition of epithelial-mesenchymal transition. Oncology Reports, 29, 2408-2414. https://doi.org/10.3892/or.2013.2370
MLA
Qian, Y., Guo, Y., Wan, H., Fan, L., Feng, Y., Ni, L., Xiang, Y., Li, Q."Angiotensin-converting enzyme 2 attenuates the metastasis of non-small cell lung cancer through inhibition of epithelial-mesenchymal transition". Oncology Reports 29.6 (2013): 2408-2414.
Chicago
Qian, Y., Guo, Y., Wan, H., Fan, L., Feng, Y., Ni, L., Xiang, Y., Li, Q."Angiotensin-converting enzyme 2 attenuates the metastasis of non-small cell lung cancer through inhibition of epithelial-mesenchymal transition". Oncology Reports 29, no. 6 (2013): 2408-2414. https://doi.org/10.3892/or.2013.2370
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