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Article

Combined use of adenoviral vector Ad5/F35-mediated APE1 siRNA enhances the therapeutic efficacy of adenoviral-mediated p53 gene transfer in hepatoma cells in vitro and in vivo

  • Authors:
    • Yanping Cun
    • Qinhong Zhang
    • Chengjie Xiong
    • Mengxia Li
    • Nan Dai
    • Shiheng Zhang
    • Dong Wang
  • View Affiliations / Copyright

    Affiliations: Cancer Center, Daping Hospital and Research Institute of Surgery, Third Military Medical University, Chongqing 400042, P.R. China, Department of Orthopedics, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, P.R. China
  • Pages: 2197-2204
    |
    Published online on: April 4, 2013
       https://doi.org/10.3892/or.2013.2384
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Abstract

Gene therapy has emerged as a novel therapeutic approach for the treatment of cancer. In order to establish a more effective therapeutic strategy against unresectable hepatocellular carcinoma (HCC), we evaluated, in the present study, the effects of combined treatment with adenoviral vector Ad5/F35-mediated APE1 siRNA (Ad5/F35-siAPE1) and adenoviral-mediated p53 gene transfer (Ad-p53) in hepatoma cells in vitro and in vivo. Infection of SMMC-7721 cells with Ad5/F35-siAPE1 resulted in a time- and dose-dependent decrease of APE1 protein, while Ad-p53 treatment led to a time- and dose-dependent increase of p53 protein expression. Ad5/F35-siAPE1 significantly enhanced the cytotoxic effect of SMMC-7721 cells to Ad-p53 in cell survival assays, associated with increased cell apoptosis. Moreover, administration of Ad5/F35-siAPE1 and Ad-p53 into nude mice resulted in tumor growth inhibition and apoptosis induction in SMMC-7721 xenografts compared to administration of either agent alone. These results suggest that combination of Ad5/F35-siAPE1 and Ad-p53 could be a promising gene therapeutic approach against human HCC.
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Copy and paste a formatted citation
Spandidos Publications style
Cun Y, Zhang Q, Xiong C, Li M, Dai N, Zhang S and Wang D: Combined use of adenoviral vector Ad5/F35-mediated APE1 siRNA enhances the therapeutic efficacy of adenoviral-mediated p53 gene transfer in hepatoma cells in vitro and in vivo. Oncol Rep 29: 2197-2204, 2013.
APA
Cun, Y., Zhang, Q., Xiong, C., Li, M., Dai, N., Zhang, S., & Wang, D. (2013). Combined use of adenoviral vector Ad5/F35-mediated APE1 siRNA enhances the therapeutic efficacy of adenoviral-mediated p53 gene transfer in hepatoma cells in vitro and in vivo. Oncology Reports, 29, 2197-2204. https://doi.org/10.3892/or.2013.2384
MLA
Cun, Y., Zhang, Q., Xiong, C., Li, M., Dai, N., Zhang, S., Wang, D."Combined use of adenoviral vector Ad5/F35-mediated APE1 siRNA enhances the therapeutic efficacy of adenoviral-mediated p53 gene transfer in hepatoma cells in vitro and in vivo". Oncology Reports 29.6 (2013): 2197-2204.
Chicago
Cun, Y., Zhang, Q., Xiong, C., Li, M., Dai, N., Zhang, S., Wang, D."Combined use of adenoviral vector Ad5/F35-mediated APE1 siRNA enhances the therapeutic efficacy of adenoviral-mediated p53 gene transfer in hepatoma cells in vitro and in vivo". Oncology Reports 29, no. 6 (2013): 2197-2204. https://doi.org/10.3892/or.2013.2384
Copy and paste a formatted citation
x
Spandidos Publications style
Cun Y, Zhang Q, Xiong C, Li M, Dai N, Zhang S and Wang D: Combined use of adenoviral vector Ad5/F35-mediated APE1 siRNA enhances the therapeutic efficacy of adenoviral-mediated p53 gene transfer in hepatoma cells in vitro and in vivo. Oncol Rep 29: 2197-2204, 2013.
APA
Cun, Y., Zhang, Q., Xiong, C., Li, M., Dai, N., Zhang, S., & Wang, D. (2013). Combined use of adenoviral vector Ad5/F35-mediated APE1 siRNA enhances the therapeutic efficacy of adenoviral-mediated p53 gene transfer in hepatoma cells in vitro and in vivo. Oncology Reports, 29, 2197-2204. https://doi.org/10.3892/or.2013.2384
MLA
Cun, Y., Zhang, Q., Xiong, C., Li, M., Dai, N., Zhang, S., Wang, D."Combined use of adenoviral vector Ad5/F35-mediated APE1 siRNA enhances the therapeutic efficacy of adenoviral-mediated p53 gene transfer in hepatoma cells in vitro and in vivo". Oncology Reports 29.6 (2013): 2197-2204.
Chicago
Cun, Y., Zhang, Q., Xiong, C., Li, M., Dai, N., Zhang, S., Wang, D."Combined use of adenoviral vector Ad5/F35-mediated APE1 siRNA enhances the therapeutic efficacy of adenoviral-mediated p53 gene transfer in hepatoma cells in vitro and in vivo". Oncology Reports 29, no. 6 (2013): 2197-2204. https://doi.org/10.3892/or.2013.2384
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