Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
June 2013 Volume 29 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June 2013 Volume 29 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Leptin induces cell proliferation and reduces cell apoptosis by activating c-myc in cervical cancer

  • Authors:
    • Yong Yuan
    • Juan Zhang
    • Lin Cai
    • Caixia Ding
    • Xiaomin Wang
    • Hongmei Chen
    • Xian Wang
    • Jun Yan
    • Jianrong Lu
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Shaanxi Cancer Hospital, Xi'an, Shaanxi 710061, P.R. China
  • Pages: 2291-2296
    |
    Published online on: April 8, 2013
       https://doi.org/10.3892/or.2013.2390
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Leptin may be involved in the pathogenesis of numerous cancer types by activation of cellular signal-transduction pathways. In this study, we analyzed the role of leptin and the mechanism(s) underlying its action in cervical carcinoma cells. Firstly, we examined the expression of leptin in 80 cases of cervical carcinoma using immunohistochemical staining. The results showed that the levels of leptin correlated significantly with the grades of cervical carcinoma. At the same time, the expression of leptin correlated positively with c-myc and its downstream gene, bcl-2. The expression of c-myc and bcl-2 was evaluated in leptin-treated HeLa cells by reverse transcription-polymerase chain reaction (RT-PCR) and western blotting. Recombinant leptin significantly activated the expression of bcl-2 and c-myc in HeLa cells. Finally, the apoptotic index, the proliferative activity and the expression levels of c-myc and bcl-2 were determined in the HeLa cells treated with silencing of leptin. We found that silencing of leptin inhibited the proliferation of HeLa cells and reduced the expression of bcl-2 and c-myc. Our data demonstrated that leptin interferes with the expression of oncogenic c-myc and anti-apoptotic bcl-2, and regulates cell turnover and facilitates the progression of cervical cancer.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Jemal A, Bray F, Center MM, et al: Global cancer statistics. CA Cancer J Clin. 61:69–90. 2011. View Article : Google Scholar

2 

MacDougald OA, Hwang CS, Fan H, Lane MD, et al: Regulated expression of the obese gene product (leptin) in white adipose tissue and 3T3-L1 adipocytes. Proc Natl Acad Sci USA. 92:9034–9037. 1995. View Article : Google Scholar : PubMed/NCBI

3 

Halaas JL, Gajiwala KS, Maffei M, et al: Weight-reducing effects of the plasma protein encoded by the obese gene. Science. 269:543–546. 1995. View Article : Google Scholar : PubMed/NCBI

4 

Huang L and Li C: Leptin: a multifunctional hormone. Cell Res. 10:81–92. 2000. View Article : Google Scholar

5 

Bouloumié A, Drexler HC, Lafontan M and Busse R: Leptin, the product of Ob gene, promotes angiogenesis. Circ Res. 83:1059–1066. 1998.PubMed/NCBI

6 

Sierra-Honigmann MR, Nath AK, Murakami C, et al: Biological action of leptin as an angiogenic factor. Science. 281:1683–1686. 1998. View Article : Google Scholar : PubMed/NCBI

7 

Somasunder P, McFadden DW, Hileman SM and Vona-Davis L: Leptin is a growth factor in cancer. J Surg Res. 116:337–349. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Bjorbaek C, Uotani S, da Silva B and Flier JS: Divergent signaling capacities of the long and short isoforms of the leptin receptor. J Biol Chem. 272:32686–32695. 1997. View Article : Google Scholar : PubMed/NCBI

9 

Tartaglia LA: The leptin receptor. J Biol Chem. 272:6093–6096. 1997. View Article : Google Scholar : PubMed/NCBI

10 

Saxena NK, Sharma D, Ding X, et al: Concomitant activation of the JAK/STAT, PI3K/AKT, and ERK signaling is involved in leptin-mediated promotion of invasion and migration of hepatocellular carcinoma cells. Cancer Res. 67:2497–2507. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Yin N, Wang D, Zhang H, et al: Molecular mechanisms involved in the growth stimulation of breast cancer cells by leptin. Cancer Res. 64:5870–5875. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Yuan Y, Wang X, Lu J, et al: Effects of leptin on biological behavior of cervical cancer Hela cells and its mechanism. Chin J Clin Exp Pathol. 28:162–165. 2012.(In Chinese).

13 

Elbashir SM, Harborth J, Weber K and Tuschl T: Analysis of gene function in somatic mammalian cells using small interfering RNAs. Methods. 26:199–213. 2002. View Article : Google Scholar : PubMed/NCBI

14 

Hoda MR, Keely SJ, Bertelsen LS, Junger WG, Dharmasena D and Barrett KE: Leptin acts as a mitogenic and antiapoptotic factor for colonic cancer cells. Br J Surg. 94:346–354. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Ogunwobi OO and Beales IL: The anti-apoptotic and growth stimulatory actions of leptin in human colon cancer cells involves activation of JNK mitogen activated protein kinase, JAK2 and PI3 kinase/Akt. Int J Colorectal Dis. 22:401–409. 2007. View Article : Google Scholar : PubMed/NCBI

16 

Pai R, Lin C, Tran T and Tarnawski A: Leptin activates STAT and ERK2 pathways and induces gastric cancer cell proliferation. Biochem Biophys Res Commun. 331:984–992. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Koda M, Sulkowska M, Wincewicz A, Kanczuga-Koda L, Musiatowicz B, Szymanska M and Sulkowski S: Expression of leptin, leptin receptor, and hypoxia-inducible factor 1 alpha in human endometrial cancer. Ann NY Acad Sci. 1095:90–98. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Choi JH, Park SH, Leung PC and Choi KC: Expression of leptin receptors and potential effects of leptin on the cell growth and activation of mitogen-activated protein kinases in ovarian cancer cells. J Clin Endocrinol Metab. 90:207–210. 2005. View Article : Google Scholar : PubMed/NCBI

19 

Ishikawa M, Kitayama J and Nagawa H: Enhanced expression of leptin and leptin receptor (OB-R) in human breast cancer. Clin Cancer Res. 10:4325–4331. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Hu X, Juneja SC, Maihle NJ and Cleary MP: Leptin - a growth factor in normal and malignant breast cells and for normal mammary gland development. J Natl Cancer Inst. 94:1704–1711. 2002. View Article : Google Scholar

21 

Mauro L, Catalano S, Bossi G, et al: Evidences that leptin up-regulates E-cadherin expression in breast cancer: effects on tumor growth and progression. Cancer Res. 67:3412–3421. 2007. View Article : Google Scholar : PubMed/NCBI

22 

Rouet-Benzineb P, Aparicio T, Guilmeau S, Pouzet C, Descatoire V, Buyse M and Bado A: Leptin counteracts sodium butyrate-induced apoptosis in human colon cancer HT-29 cells via NF-κB signaling. J Biol Chem. 279:16495–16502. 2004.PubMed/NCBI

23 

Escobar-Morreale HF and San Millán JL: Abdominal adiposity and the polycystic ovary syndrome. Trends Endocrinol Metab. 18:266–272. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Sharma D, Saxena NK, Vertino PM and Anania FA: Leptin promotes the proliferative response and invasiveness in human endometrial cancer cells by activating multiple signal-transduction pathways. Endocr Relat Cancer. 13:629–640. 2006. View Article : Google Scholar

25 

Sheiness D, Fanshier L and Bishop JM: Identification of nucleotide sequences which may encode the oncogenic capacity of avian retrovirus MC29. J Virol. 28:600–610. 1978.PubMed/NCBI

26 

Persson H and Leder P: Nuclear localization and DNA binding properties of a protein expressed by human c-myc oncogene. Science. 225:718–721. 1984. View Article : Google Scholar : PubMed/NCBI

27 

Vita M and Henriksson M: The Myc oncoprotein as a therapeutic target for human cancer. Semin Cancer Biol. 16:318–330. 2006. View Article : Google Scholar : PubMed/NCBI

28 

O’Connell BC, Cheung AF, Simkevich CP, Tam W, Ren X, Mateyak MK and Sedivy JM: A large scale genetic analysis of c-Myc-regulated gene expression patterns. J Biol Chem. 278:12563–12573. 2003.PubMed/NCBI

29 

Nesbit CE, Tersak JM and Prochownik EV: MYC oncogenes and human neoplastic disease. Oncogene. 18:3004–3016. 1999. View Article : Google Scholar : PubMed/NCBI

30 

Chung HJ and Levens D: c-myc expression: keep the noise down! Mol Cells. 20:157–166. 2005.PubMed/NCBI

31 

Skirnisdóttir I, Seidal T, Gerdin E and Sorbe B: The prognostic importance of p53, bcl-2, and bax in early stage epithelial ovarian carcinoma treated with adjuvant chemotherapy. Int J Gynecol Cancer. 12:265–276. 2002.PubMed/NCBI

32 

Oltvai ZN, Milliman CL and Korsmeyer SJ: Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell. 74:609–619. 1993. View Article : Google Scholar : PubMed/NCBI

33 

Chung TK, Cheung TH, Lo WK, et al: Expression of apoptotic regulators and their significance in cervical cancer. Cancer Lett. 180:63–68. 2002. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Yuan Y, Zhang J, Cai L, Ding C, Wang X, Chen H, Wang X, Yan J and Lu J: Leptin induces cell proliferation and reduces cell apoptosis by activating c-myc in cervical cancer. Oncol Rep 29: 2291-2296, 2013.
APA
Yuan, Y., Zhang, J., Cai, L., Ding, C., Wang, X., Chen, H. ... Lu, J. (2013). Leptin induces cell proliferation and reduces cell apoptosis by activating c-myc in cervical cancer. Oncology Reports, 29, 2291-2296. https://doi.org/10.3892/or.2013.2390
MLA
Yuan, Y., Zhang, J., Cai, L., Ding, C., Wang, X., Chen, H., Wang, X., Yan, J., Lu, J."Leptin induces cell proliferation and reduces cell apoptosis by activating c-myc in cervical cancer". Oncology Reports 29.6 (2013): 2291-2296.
Chicago
Yuan, Y., Zhang, J., Cai, L., Ding, C., Wang, X., Chen, H., Wang, X., Yan, J., Lu, J."Leptin induces cell proliferation and reduces cell apoptosis by activating c-myc in cervical cancer". Oncology Reports 29, no. 6 (2013): 2291-2296. https://doi.org/10.3892/or.2013.2390
Copy and paste a formatted citation
x
Spandidos Publications style
Yuan Y, Zhang J, Cai L, Ding C, Wang X, Chen H, Wang X, Yan J and Lu J: Leptin induces cell proliferation and reduces cell apoptosis by activating c-myc in cervical cancer. Oncol Rep 29: 2291-2296, 2013.
APA
Yuan, Y., Zhang, J., Cai, L., Ding, C., Wang, X., Chen, H. ... Lu, J. (2013). Leptin induces cell proliferation and reduces cell apoptosis by activating c-myc in cervical cancer. Oncology Reports, 29, 2291-2296. https://doi.org/10.3892/or.2013.2390
MLA
Yuan, Y., Zhang, J., Cai, L., Ding, C., Wang, X., Chen, H., Wang, X., Yan, J., Lu, J."Leptin induces cell proliferation and reduces cell apoptosis by activating c-myc in cervical cancer". Oncology Reports 29.6 (2013): 2291-2296.
Chicago
Yuan, Y., Zhang, J., Cai, L., Ding, C., Wang, X., Chen, H., Wang, X., Yan, J., Lu, J."Leptin induces cell proliferation and reduces cell apoptosis by activating c-myc in cervical cancer". Oncology Reports 29, no. 6 (2013): 2291-2296. https://doi.org/10.3892/or.2013.2390
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team