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June 2013 Volume 29 Issue 6

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International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

Upregulation of the eIF4E signaling pathway contributes to the progression of gastric cancer, and targeting eIF4E by perifosine inhibits cell growth

  • Authors:
    • Song Liang
    • Renhua Guo
    • Zhihong Zhang
    • Dongxiao Liu
    • Hao Xu
    • Zekuan Xu
    • Xuerong Wang
    • Li Yang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, P.R. China, Department of Medical Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, P.R. China, Department of Pathology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, P.R. China, Department of General Surgery, The Second Affiliated Hospital of South-east University, The Second Hospital of Nanjing, Nanjing, P.R. China, Department of Pharmacology, Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China
  • Pages: 2422-2430
    |
    Published online on: April 9, 2013
       https://doi.org/10.3892/or.2013.2397
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Abstract

The increase of eukaryotic translation initiation factor 4E (eIF4E) expression is frequently observed in several types of cancer, making eIF4E an attractive anticancer drug target. However, the role of eIF4E in gastric cancer pathogenesis remains unclear. Perifosine is a bioavailable alkylphospholipid exhibiting antitumor activity in a series of cancer types. In this study, gastric cancer cell lines were selected to explore the role of eIF4E as a potential target for treating human gastric cancer. The expression of total eIF4E (T-eIF4E)and phosphorylated eIF4E (p-eIF4E) in gastric cancer samples was detected by immunohistochemical assay. RNA interference was used to silence eIF4E expression. Sulforhodamine B assay was performed to evaluate tumor cell viability. Colony formation assay was used to examine the effects of eIF4E small interfering RNA (siRNA) or perifosine on colony formation. The mRNA levels of eIF4E were analyzed by qRT-PCR and western blot analysis was carried out to evaluate the expression of Akt and eIF4E. The results showed that increased expression levels of T-eIF4E and p-eIF4E were found in gastric cancer tissues and cells. Reduced eIF4E expression blocked the proliferation of gastric cancer cells. Perifosine downregulated the T-eIF4E and p-eIF4E levels in a dose- and time-dependent manner; it also inhibited the growth of gastric cancer cells. Moreover, this inhibitory effect was significantly enhanced by the combination of eIF4E siRNA and perifosine treatments. Our results indicate that eIF4E gene silencing can inhibit tumor cell growth, and eIF4E can be developed as a promising therapeutic target for gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Liang S, Guo R, Zhang Z, Liu D, Xu H, Xu Z, Wang X and Yang L: Upregulation of the eIF4E signaling pathway contributes to the progression of gastric cancer, and targeting eIF4E by perifosine inhibits cell growth. Oncol Rep 29: 2422-2430, 2013.
APA
Liang, S., Guo, R., Zhang, Z., Liu, D., Xu, H., Xu, Z. ... Yang, L. (2013). Upregulation of the eIF4E signaling pathway contributes to the progression of gastric cancer, and targeting eIF4E by perifosine inhibits cell growth. Oncology Reports, 29, 2422-2430. https://doi.org/10.3892/or.2013.2397
MLA
Liang, S., Guo, R., Zhang, Z., Liu, D., Xu, H., Xu, Z., Wang, X., Yang, L."Upregulation of the eIF4E signaling pathway contributes to the progression of gastric cancer, and targeting eIF4E by perifosine inhibits cell growth". Oncology Reports 29.6 (2013): 2422-2430.
Chicago
Liang, S., Guo, R., Zhang, Z., Liu, D., Xu, H., Xu, Z., Wang, X., Yang, L."Upregulation of the eIF4E signaling pathway contributes to the progression of gastric cancer, and targeting eIF4E by perifosine inhibits cell growth". Oncology Reports 29, no. 6 (2013): 2422-2430. https://doi.org/10.3892/or.2013.2397
Copy and paste a formatted citation
x
Spandidos Publications style
Liang S, Guo R, Zhang Z, Liu D, Xu H, Xu Z, Wang X and Yang L: Upregulation of the eIF4E signaling pathway contributes to the progression of gastric cancer, and targeting eIF4E by perifosine inhibits cell growth. Oncol Rep 29: 2422-2430, 2013.
APA
Liang, S., Guo, R., Zhang, Z., Liu, D., Xu, H., Xu, Z. ... Yang, L. (2013). Upregulation of the eIF4E signaling pathway contributes to the progression of gastric cancer, and targeting eIF4E by perifosine inhibits cell growth. Oncology Reports, 29, 2422-2430. https://doi.org/10.3892/or.2013.2397
MLA
Liang, S., Guo, R., Zhang, Z., Liu, D., Xu, H., Xu, Z., Wang, X., Yang, L."Upregulation of the eIF4E signaling pathway contributes to the progression of gastric cancer, and targeting eIF4E by perifosine inhibits cell growth". Oncology Reports 29.6 (2013): 2422-2430.
Chicago
Liang, S., Guo, R., Zhang, Z., Liu, D., Xu, H., Xu, Z., Wang, X., Yang, L."Upregulation of the eIF4E signaling pathway contributes to the progression of gastric cancer, and targeting eIF4E by perifosine inhibits cell growth". Oncology Reports 29, no. 6 (2013): 2422-2430. https://doi.org/10.3892/or.2013.2397
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