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Article

HSPA9 overexpression inhibits apoptin-induced apoptosis in the HepG2 cell line

  • Authors:
    • Chuanmei Peng
    • Pu Yang
    • Yingbo Cui
    • Ming He
    • Leilei Liang
    • Yong Di
  • View Affiliations / Copyright

    Affiliations: Clinical Laboratory of Yanan Hospital of Kunming, Kunming 650051, P.R. China, Biomechanics Institute, Department of Biomedical Engineering, School of Biological Science and Engineering, South China University of Technology, Guangzhou 510006, P.R. China, Department of Biochemistry and Molecular Biology, Kunming Medical University, Kunming 650500, P.R. China
  • Pages: 2431-2437
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    Published online on: April 9, 2013
       https://doi.org/10.3892/or.2013.2399
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Abstract

Apoptin, a small protein derived from chicken anemia virus, possesses the capacity to specifically kill tumor cells while leaving normal cells intact. Previous studies have indicated that the subcellular localization of apoptin appears to be crucial for this tumor-selective activity. Apoptin resides in the cytoplasm of normal cells; however, in cancer cells it translocates into the nucleus. In the present study, purified prokaryotic native His-apoptin served as a bait for capturing apoptin-associated proteins in both a hepatoma carcinoma cell line (HepG2) and a human fetal liver cell line (L-02). The captured proteins obtained from a pull-down assay were separated by two-dimensional gel electrophoresis. Mass spectrometry was employed to detect the effect of HSPA9 overexpression (one of the interacting proteins with apoptin in vitro) and downregulation of HSPA9 on HepG2 cells. The data revealed that HSPA9 overexpression resulted in partial distribution of apoptin in the cytoplasm. Notably, HSPA9 overexpression markedly decreased the apoptosis rate of HepG2 cells from 41.2 to 31.7%, while the downregulation of HSPA9 using small interfering RNA significantly enhanced the apoptosis of HepG2 cells. Our results suggest new insights into the localization mechanism of apoptin which is tightly associated with HSPA9 overexpression and its crucial role in cellular apoptosis both in a tumor cell line (HepG2) and a normal cell line (L-02). These findings shed new light on the elucidation of the underlying mechanism of anticancer action of apoptin.
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Copy and paste a formatted citation
Spandidos Publications style
Peng C, Yang P, Cui Y, He M, Liang L and Di Y: HSPA9 overexpression inhibits apoptin-induced apoptosis in the HepG2 cell line. Oncol Rep 29: 2431-2437, 2013.
APA
Peng, C., Yang, P., Cui, Y., He, M., Liang, L., & Di, Y. (2013). HSPA9 overexpression inhibits apoptin-induced apoptosis in the HepG2 cell line. Oncology Reports, 29, 2431-2437. https://doi.org/10.3892/or.2013.2399
MLA
Peng, C., Yang, P., Cui, Y., He, M., Liang, L., Di, Y."HSPA9 overexpression inhibits apoptin-induced apoptosis in the HepG2 cell line". Oncology Reports 29.6 (2013): 2431-2437.
Chicago
Peng, C., Yang, P., Cui, Y., He, M., Liang, L., Di, Y."HSPA9 overexpression inhibits apoptin-induced apoptosis in the HepG2 cell line". Oncology Reports 29, no. 6 (2013): 2431-2437. https://doi.org/10.3892/or.2013.2399
Copy and paste a formatted citation
x
Spandidos Publications style
Peng C, Yang P, Cui Y, He M, Liang L and Di Y: HSPA9 overexpression inhibits apoptin-induced apoptosis in the HepG2 cell line. Oncol Rep 29: 2431-2437, 2013.
APA
Peng, C., Yang, P., Cui, Y., He, M., Liang, L., & Di, Y. (2013). HSPA9 overexpression inhibits apoptin-induced apoptosis in the HepG2 cell line. Oncology Reports, 29, 2431-2437. https://doi.org/10.3892/or.2013.2399
MLA
Peng, C., Yang, P., Cui, Y., He, M., Liang, L., Di, Y."HSPA9 overexpression inhibits apoptin-induced apoptosis in the HepG2 cell line". Oncology Reports 29.6 (2013): 2431-2437.
Chicago
Peng, C., Yang, P., Cui, Y., He, M., Liang, L., Di, Y."HSPA9 overexpression inhibits apoptin-induced apoptosis in the HepG2 cell line". Oncology Reports 29, no. 6 (2013): 2431-2437. https://doi.org/10.3892/or.2013.2399
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