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Article

Promotive effects of cell proliferation and chromosomal instability induced by tribbles-related protein 3 in mouse mammary tumor cells

  • Authors:
    • Yuto Sakai
    • Katsumi Fukamachi
    • Mitsuru Futakuchi
    • Hidetoshi Hayashi
    • Masumi Suzui
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Toxicology, Nagoya City University Graduate School of Medical Sciences and Medical School, Nagoya 467-8601, Japan, Department of Drug Metabolism and Disposition, Nagoya City University Graduate School of Pharmaceutical Sciences, Nagoya 467-8603, Japan
  • Pages: 64-70
    |
    Published online on: April 30, 2013
       https://doi.org/10.3892/or.2013.2441
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Abstract

Tribbles-related protein 3 (TRB3) has been shown to be a crucial modulator of tumorigenesis. However, the precise role and the functional morphology of TRB3 are not clearly understood. To elucidate these enigmas we established the cell line, M2TRB3, by introducing the human TRB3 gene and protein in Cl66M2 (M2) mouse mammary tumor cells. This cell line stably expressed the TRB3 gene and protein. After 72 h of cell culture, there was a 34% increase in the growth of M2TRB3 cells compared to the control M2 mock cells. The mean volume of the tumors originating from the M2TRB3 cells was significantly increased by 38% when compared to the mean volume of the M2 mock tumors, and the proliferating cell nuclear antigen (PCNA) labeling index in the M2TRB3 tumors was higher when compared to that of the M2 and M2 mock cells. In the tumor tissue samples, the mean diameter of nuclei in the M2TRB3 tumor cells (9.4±0.3 µm) showed a significant increase compared to that of the M2 mock tumor cells (7.0±0.2 µm). M2TRB3 cells also showed a marked increase in the population of tetraploid or octaploid nuclei compared to M2 mock cells bearing mainly either diploid or tetraploid nuclei. Western blot analysis revealed the overexpression of cyclin B1 and cyclin D1 in M2TRB3 cells when compared to that in the M2 mock cells. These novel findings provide further evidence that TRB3 promotes cell proliferation and chromosomal instability by causing polyploidization during development.
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Copy and paste a formatted citation
Spandidos Publications style
Sakai Y, Fukamachi K, Futakuchi M, Hayashi H and Suzui M: Promotive effects of cell proliferation and chromosomal instability induced by tribbles-related protein 3 in mouse mammary tumor cells. Oncol Rep 30: 64-70, 2013.
APA
Sakai, Y., Fukamachi, K., Futakuchi, M., Hayashi, H., & Suzui, M. (2013). Promotive effects of cell proliferation and chromosomal instability induced by tribbles-related protein 3 in mouse mammary tumor cells. Oncology Reports, 30, 64-70. https://doi.org/10.3892/or.2013.2441
MLA
Sakai, Y., Fukamachi, K., Futakuchi, M., Hayashi, H., Suzui, M."Promotive effects of cell proliferation and chromosomal instability induced by tribbles-related protein 3 in mouse mammary tumor cells". Oncology Reports 30.1 (2013): 64-70.
Chicago
Sakai, Y., Fukamachi, K., Futakuchi, M., Hayashi, H., Suzui, M."Promotive effects of cell proliferation and chromosomal instability induced by tribbles-related protein 3 in mouse mammary tumor cells". Oncology Reports 30, no. 1 (2013): 64-70. https://doi.org/10.3892/or.2013.2441
Copy and paste a formatted citation
x
Spandidos Publications style
Sakai Y, Fukamachi K, Futakuchi M, Hayashi H and Suzui M: Promotive effects of cell proliferation and chromosomal instability induced by tribbles-related protein 3 in mouse mammary tumor cells. Oncol Rep 30: 64-70, 2013.
APA
Sakai, Y., Fukamachi, K., Futakuchi, M., Hayashi, H., & Suzui, M. (2013). Promotive effects of cell proliferation and chromosomal instability induced by tribbles-related protein 3 in mouse mammary tumor cells. Oncology Reports, 30, 64-70. https://doi.org/10.3892/or.2013.2441
MLA
Sakai, Y., Fukamachi, K., Futakuchi, M., Hayashi, H., Suzui, M."Promotive effects of cell proliferation and chromosomal instability induced by tribbles-related protein 3 in mouse mammary tumor cells". Oncology Reports 30.1 (2013): 64-70.
Chicago
Sakai, Y., Fukamachi, K., Futakuchi, M., Hayashi, H., Suzui, M."Promotive effects of cell proliferation and chromosomal instability induced by tribbles-related protein 3 in mouse mammary tumor cells". Oncology Reports 30, no. 1 (2013): 64-70. https://doi.org/10.3892/or.2013.2441
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