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Article

CD133+ subpopulation of the HT1080 human fibrosarcoma cell line exhibits cancer stem-like characteristics

  • Authors:
    • Bao‑Hua Feng
    • Ai‑Guo Liu
    • Wen‑Guang Gu
    • Liang Deng
    • Xian‑Gyang Cheng
    • Tie‑Jun Tong
    • Hong‑Zhi Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China
  • Pages: 815-823
    |
    Published online on: May 23, 2013
       https://doi.org/10.3892/or.2013.2486
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Abstract

The cancer stem cell (CSC) theory holds that a minority population within tumors possesses stem cell properties of self-renewal and multilineage differentiation capacity and provides the initiating cells from which tumors are derived and sustained. However, verifying the existence of these CSCs has been a significant challenge. The CD133 antigen is a pentaspan membrane glycoprotein proposed to be a CSC marker for cancer-initiating subpopulations in the brain, colon and various other tissues. Here, CD133+ cells were obtained and characterized from the HT1080 cell line to determine the utility of this marker for isolating CSCs from human fibrosarcoma cells. In this study, CD133+ cells were separated from HT1080 cells using magnetic beads and characterized for their proliferation rate and resistance to chemotherapeutic drugs, cisplatin and doxorubicin, by MTS assay. Relative expression of tumor-associated genes Sox2, Oct3/4, Nanog, c-Myc, Bmi-1 and ABCG2 was measured by real-time polymerase chain reaction (PCR). Clonal sphere formation and the ability of CD133+ cells to initiate tumors in BALB/c nude mice was also evaluated. We found that CD133+ cells showed a high proliferation rate, increased resistance to chemotherapy drugs and overexpression of tumor-associated genes compared with these features in CD133- cells. Additionally, CD133+ cells were able to form spherical clusters in serum-free medium with high clonogenic efficiency, indicating a significantly greater tumor-initiating potential when compared with CD133- cells. These findings indicate that CD133+ cells identified within the HT1080 human fibrosarcoma cell line possess many CSC properties and may facilitate the development of improved therapies for fibrosarcoma.
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Copy and paste a formatted citation
Spandidos Publications style
Feng BH, Liu AG, Gu WG, Deng L, Cheng XG, Tong TJ and Zhang HZ: CD133+ subpopulation of the HT1080 human fibrosarcoma cell line exhibits cancer stem-like characteristics. Oncol Rep 30: 815-823, 2013.
APA
Feng, B., Liu, A., Gu, W., Deng, L., Cheng, X., Tong, T., & Zhang, H. (2013). CD133+ subpopulation of the HT1080 human fibrosarcoma cell line exhibits cancer stem-like characteristics. Oncology Reports, 30, 815-823. https://doi.org/10.3892/or.2013.2486
MLA
Feng, B., Liu, A., Gu, W., Deng, L., Cheng, X., Tong, T., Zhang, H."CD133+ subpopulation of the HT1080 human fibrosarcoma cell line exhibits cancer stem-like characteristics". Oncology Reports 30.2 (2013): 815-823.
Chicago
Feng, B., Liu, A., Gu, W., Deng, L., Cheng, X., Tong, T., Zhang, H."CD133+ subpopulation of the HT1080 human fibrosarcoma cell line exhibits cancer stem-like characteristics". Oncology Reports 30, no. 2 (2013): 815-823. https://doi.org/10.3892/or.2013.2486
Copy and paste a formatted citation
x
Spandidos Publications style
Feng BH, Liu AG, Gu WG, Deng L, Cheng XG, Tong TJ and Zhang HZ: CD133+ subpopulation of the HT1080 human fibrosarcoma cell line exhibits cancer stem-like characteristics. Oncol Rep 30: 815-823, 2013.
APA
Feng, B., Liu, A., Gu, W., Deng, L., Cheng, X., Tong, T., & Zhang, H. (2013). CD133+ subpopulation of the HT1080 human fibrosarcoma cell line exhibits cancer stem-like characteristics. Oncology Reports, 30, 815-823. https://doi.org/10.3892/or.2013.2486
MLA
Feng, B., Liu, A., Gu, W., Deng, L., Cheng, X., Tong, T., Zhang, H."CD133+ subpopulation of the HT1080 human fibrosarcoma cell line exhibits cancer stem-like characteristics". Oncology Reports 30.2 (2013): 815-823.
Chicago
Feng, B., Liu, A., Gu, W., Deng, L., Cheng, X., Tong, T., Zhang, H."CD133+ subpopulation of the HT1080 human fibrosarcoma cell line exhibits cancer stem-like characteristics". Oncology Reports 30, no. 2 (2013): 815-823. https://doi.org/10.3892/or.2013.2486
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