miR-708 acts as a tumor suppressor in human glioblastoma cells

Guo,Pin; Lan,Jin; Ge,Jianwei; Nie,Quanmin; Mao,Qing; Qiu,Yongming

doi:10.3892/or.2013.2526

miR-708 acts as a tumor suppressor in human glioblastoma cells

Authors:
- Pin Guo
- Jin Lan
- Jianwei Ge
- Quanmin Nie
- Qing Mao
- Yongming Qiu
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Affiliations: Department of Neurosurgery, Ren Ji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, P.R. China, Shanghai Institute of Head Trauma, Shanghai 200127, P.R. China
Published online on: June 7, 2013 https://doi.org/10.3892/or.2013.2526
Pages: 870-876

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Abstract

Glioblastoma (GBM) is one of the most lethal forms of human cancer, and new clinical biomarkers and therapeutic targets are urgently required. microRNAs (miRNAs) are small, non-coding RNAs that negatively regulate gene expression at the post-transcriptional and/or translational level by binding the 3' untranslated regions (3' UTRs) of target mRNAs. The dysregulated expression of several miRNAs has been reported to modulate glioma progression. In the present study, we defined the expression and function of miR-708, which, based on real-time PCR analysis, were downregulated in GBM cells. The overexpression of miR-708 inhibited cell proliferation and invasion and induced apoptosis in the human GBM cell lines A172 and T98G. Furthermore, the overexpression of miR-708 reduced the expression of Akt1, CCND1, MMP2, EZH2, Parp-1 and Bcl2 in A172 and T98G cells. Taken together, our study suggests that miR-708 affects GBM cell proliferation and invasion, and induces apoptosis. It is suggested that miR-708 may play an important role as a tumor suppressor in GBM and it may be an attractive target for therapeutic intervention in GBM.

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