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Article

Patrinia scabiosaefolia induces mitochondrial-dependent apoptosis in a mouse model of colorectal cancer

  • Authors:
    • Liya Liu
    • Aling Shen
    • Youqin Chen
    • Lihui Wei
    • Jiumao Lin
    • Thomas J. Sferra
    • Zhenfeng Hong
    • Jun Peng
  • View Affiliations / Copyright

    Affiliations: Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Minhou Shangjie, Fuzhou, Fujian 350122, P.R. China, Rainbow Babies and Children's Hospital, Case Western Reserve University School of Medicine, Cleveland, OH 44106, USA, Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Minhou Shangjie, Fuzhou, Fujian 350122, P.R. China
  • Pages: 897-903
    |
    Published online on: June 7, 2013
       https://doi.org/10.3892/or.2013.2528
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Abstract

Disrupted apoptosis not only confers a survival advantage to cancer cells but also causes resistance to chemotherapies. Therefore, inducing cell apoptosis has become a promising strategy for anticancer treatment. Patrinia scabiosaefolia (PS) has long been used to clinically treat various types of malignancies including colorectal cancer (CRC). However, the precise mechanism of its tumoricidal activity remains largely unclear. Using a CRC mouse xenograft model and a human colon carcinoma cell line, HT-29, in the present study, we evaluated the antitumor activities of an ethanol extract of Patrinia scabiosaefolia (EEPS), and investigated the underlying molecular mechanisms. We found that EEPS inhibited CRC growth both in vivo and in vitro, without apparent adverse side-effects. Moreover, EEPS treatment promoted apoptosis in CRC tumor tissues and in HT-29 cells, suggesting that the inhibitory effect of EEPS on tumor growth was due to its pro-apoptotic activity. Furthermore, EEPS treatment inhibited the expression of anti-apoptotic Bcl-2 but enhanced pro-apoptotic Bax expression at both transcriptional and translational levels. Finally, EEPS induced the loss of mitochondrial membrane potential and activation of caspase-9 and -3 in HT-29 cells. Taken together, data in this study suggest that induction of cancer cell apoptosis via the mitochondrial-dependent pathway may be one of the mechanisms whereby PS exerts anticancer activity.
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Copy and paste a formatted citation
Spandidos Publications style
Liu L, Shen A, Chen Y, Wei L, Lin J, Sferra TJ, Hong Z and Peng J: Patrinia scabiosaefolia induces mitochondrial-dependent apoptosis in a mouse model of colorectal cancer. Oncol Rep 30: 897-903, 2013.
APA
Liu, L., Shen, A., Chen, Y., Wei, L., Lin, J., Sferra, T.J. ... Peng, J. (2013). Patrinia scabiosaefolia induces mitochondrial-dependent apoptosis in a mouse model of colorectal cancer. Oncology Reports, 30, 897-903. https://doi.org/10.3892/or.2013.2528
MLA
Liu, L., Shen, A., Chen, Y., Wei, L., Lin, J., Sferra, T. J., Hong, Z., Peng, J."Patrinia scabiosaefolia induces mitochondrial-dependent apoptosis in a mouse model of colorectal cancer". Oncology Reports 30.2 (2013): 897-903.
Chicago
Liu, L., Shen, A., Chen, Y., Wei, L., Lin, J., Sferra, T. J., Hong, Z., Peng, J."Patrinia scabiosaefolia induces mitochondrial-dependent apoptosis in a mouse model of colorectal cancer". Oncology Reports 30, no. 2 (2013): 897-903. https://doi.org/10.3892/or.2013.2528
Copy and paste a formatted citation
x
Spandidos Publications style
Liu L, Shen A, Chen Y, Wei L, Lin J, Sferra TJ, Hong Z and Peng J: Patrinia scabiosaefolia induces mitochondrial-dependent apoptosis in a mouse model of colorectal cancer. Oncol Rep 30: 897-903, 2013.
APA
Liu, L., Shen, A., Chen, Y., Wei, L., Lin, J., Sferra, T.J. ... Peng, J. (2013). Patrinia scabiosaefolia induces mitochondrial-dependent apoptosis in a mouse model of colorectal cancer. Oncology Reports, 30, 897-903. https://doi.org/10.3892/or.2013.2528
MLA
Liu, L., Shen, A., Chen, Y., Wei, L., Lin, J., Sferra, T. J., Hong, Z., Peng, J."Patrinia scabiosaefolia induces mitochondrial-dependent apoptosis in a mouse model of colorectal cancer". Oncology Reports 30.2 (2013): 897-903.
Chicago
Liu, L., Shen, A., Chen, Y., Wei, L., Lin, J., Sferra, T. J., Hong, Z., Peng, J."Patrinia scabiosaefolia induces mitochondrial-dependent apoptosis in a mouse model of colorectal cancer". Oncology Reports 30, no. 2 (2013): 897-903. https://doi.org/10.3892/or.2013.2528
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