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Article

Glutathione and glutathione peroxidase expression in breast cancer: An immunohistochemical and molecular study

  • Authors:
    • Bruna Victorasso Jardim
    • Marina Gobbe Moschetta
    • Camila Leonel
    • Gabriela Bottaro Gelaleti
    • Vitor Rafael Regiani
    • Lívia Carvalho Ferreira
    • Juliana Ramos Lopes
    • Debora Ap. Pires de Campos Zuccari
  • View Affiliations / Copyright

    Affiliations: Department of Biology, Sao Paulo State University - UNESP/IBILCE, 15090-000 São José do Rio Preto, SP, Brazil, Department of Molecular Biology, Laboratory of Molecular Research in Cancer (LIMC), Faculty of Medicine of Sao Jose do Rio Preto (FAMERP), 15090-000 São José do Rio Preto, SP, Brazil
  • Pages: 1119-1128
    |
    Published online on: June 13, 2013
       https://doi.org/10.3892/or.2013.2540
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Abstract

The use of prognostic markers for breast cancer allows therapeutic strategies to be defined more efficiently. The expression of glutathione (GSH) and glutathione peroxidase (GPX) in tumor cells has been evaluated as a predictor of prognosis and response to cytotoxic treatments. Its immunoexpression was assessed in 63 women diagnosed with invasive ductal carcinoma in a retrospective study. The results showed that high GSH expression was associated with tumors negative for the estrogen receptor (ER) (P<0.05), and GPX expression was associated with tumors negative for the progesterone receptor (PR) and patient mortality. Focusing on the 37 patients who received adjuvant chemotherapy/radiotherapy (Group I), high expression of GPX was associated with a high rate of patient mortality (P<0.05). The 19 patients who received only adjuvant chemotherapy (Group II) showed high expression of GSH in relation to metastasis (P<0.05). In addition, high levels of GPX expression were significantly associated with a shorter overall survival (P<0.05). To confirm this, the expression of precursor genes of GSH [glutamate cysteine ligase (GCLC) and glutathione synthetase (GSS)] and the GPX gene was analyzed using quantitative PCR in cultured neoplastic mammary cells treated with doxorubicin. Doxorubicin treatment was able to eliminate tumor cells without alterations in the gene expression of GSS, but led to underexpression of the GCLC and GPX genes. Our results suggest that high levels of GPX may be related to the development of resistance to chemotherapy in these tumors, response to treatment and the clinical course of the breast cancer patients.
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1 

Gonzalez-Angulo AM, Moraes-Vasquez F and Hortobagyl GN: Overview of resistance to systemic therapy in patients with breast cancer. Adv Exp Med Biol. 608:1–22. 2007. View Article : Google Scholar : PubMed/NCBI

2 

Instituto Nacional do Câncer (INCA). Estimativas da incidência e mortalidade por câncer no Brasil. Ministério da Saúde Rio de Janeiro. http://www.inca.gov.br/estimativa/2010/index.asp?link=conteudo_view.asp&ID=5. Accessed Nov. 20, 2011

3 

Gralow J, Ozols RF, Bajorin DF, et al: Clinical cancer advances 2007: major research advances in cancer treatment, prevention, and screening - a report from the American Society of Clinical Oncology. J Clin Oncol. 26:313–325. 2008. View Article : Google Scholar : PubMed/NCBI

4 

Hicks DG and Kulkarni S: Trastuzumab as adjuvant therapy for early breast cancer. Arch Pathol Lab Med. 132:1008–1015. 2008.PubMed/NCBI

5 

vant’t Veer LJ, Paik S and Hayes DF: Gene expression profiling of breast cancer: a new tumor marker. J Clin Oncol. 23:1631–1635. 2005.PubMed/NCBI

6 

Pedersen L, Gunnarsdottir KA, Rasmussen BB, et al: The prognostic influence of multifocality in breast cancer patients. Breast. 13:188–193. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Duffy MJ and Crown J: A personalized approach to cancer treatment: how biomarkers can help. Clin Chem. 54:1770–1779. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Thomas E and Berner G: Prognostic and predictive implications of HER2 status for breast cancer patients. Eur J Oncol Nurs. 4:10–17. 2000. View Article : Google Scholar : PubMed/NCBI

9 

Duffy MJ, O’Donovan N and Crown J: Use of molecular markers for predicting therapy response in cancer patients. Cancer Treat Rev. 37:151–159. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Pastore A, Federici G, Bertini E and Piemonte F: Analysis of glutathione: implication in redox and detoxification. Clin Chem Acta. 333:19–39. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Townsend DM and Tew KD: The role of glutathione-S-transferase in anti-cancer drug resistance. Oncogene. 22:7369–7375. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Jardim BV, Moschetta MG, Gelaleti GB, et al: Glutathione transferase pi (GSTpi) expression in breast cancer: An immunohistochemical and molecular study. Acta Histochem. 114:510–517. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Huber PC, Almeida WP and Fatima A: Glutationa e enzimas relacionadas: papel biológico e importância em processos patológicos. Quím Nova. 31:1170–1179. 2008.

14 

Carnicer MJ, Bernardini S, Bellincampi L, et al: Role of γ-glutamyl cysteine synthetase (γ-GCS) gene expression as marker of drug sensitivity in acute myeloid leukemias. Clin Chem Acta. 365:342–345. 2006.

15 

Uchida M, Sugaya M, Kanamaru T and Hisatomi H: Alternative RNA splicing in expression of the glutathione synthetase gene in human cells. Mol Biol Rep. 37:2105–2109. 2010. View Article : Google Scholar : PubMed/NCBI

16 

Valko M, Leibfritz D, Moncol J, et al: Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 39:44–84. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Ballatori N, Krance SM, Notenboom S, et al: Glutathione dysregulation and the etiology and progression of human diseases. Biol Chem. 390:191–214. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Kumaraguruparan R, Balachandran C, Manohar BM and Nagini S: Altered oxidant-antioxidant profile in canine mammary tumours. Vet Res Commun. 29:287–296. 2005. View Article : Google Scholar : PubMed/NCBI

19 

Rajneesh CP, Manimaran A, Sasikala KR and Adaikappan P: Lipid peroxidation and antioxidant status in patients with breast cancer. Singapore Med J. 49:640–643. 2008.PubMed/NCBI

20 

Kasapović J, Pejić S, Stojiljković V, et al: Antioxidant status and lipid peroxidation in the blood of breast cancer patients of different ages after chemotherapy with 5-fluorouracil, doxorubicin and cyclophosphamide. Clin Biochem. 43:1287–1293. 2010.PubMed/NCBI

21 

Hammond ME, Hayes DF, Dowsett M, et al: American Society of Clinical Oncology/College of American Pathologists guideline recommendations for immunohistochemical testing of estrogen and progesterone receptors in breast cancer. J Clin Oncol. 28:2784–2795. 2010. View Article : Google Scholar

22 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−ΔΔCT) method. Methods. 25:402–408. 2001.

23 

Yeh CC, Hou MF, Wu SH, et al: A study of glutathione status in the blood and tissues of patients with breast cancer. Cell Biochem Funct. 24:555–559. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Kasapović J, Pejić S, Todorović A, et al: Antioxidant status and lipid peroxidation in the blood of breast cancer patients of different ages. Cell Biochem Funct. 26:723–730. 2008.

25 

Fernandes RC, Bevilacqua JL, Soares IC, et al: Coordinated expression of ER, PR and HER2 define different prognostic subtypes among poorly differentiated breast carcinomas. Histopathology. 55:346–352. 2009. View Article : Google Scholar

26 

Basu S, Chen W, Tchou J, et al: Comparison of triple-negative and estrogen receptor-positive/progesterone receptor-positive/HER2-negative breast carcinoma using quantitative fluorine-18 fluorodeoxyglucose/positron emission tomography imaging parameters: a potentially useful method for disease characterization. Cancer. 112:995–1000. 2008.

27 

Murawaki Y, Tsuchiya H, Kanbe T, et al: Aberrant expression of selenoproteins in the progression of colorectal cancer. Cancer Lett. 259:218–230. 2008. View Article : Google Scholar : PubMed/NCBI

28 

Li S, Yan T, Yang JQ, et al: The role of cellular glutathione peroxidase redox regulation in the suppression of tumor cell growth by manganese superoxide dismutase. Cancer Res. 60:3927–3939. 2000.PubMed/NCBI

29 

Estrela JM, Ortega A and Obrador E: Glutathione in cancer biology and therapy. Crit Rev Clin Lab Sci. 43:143–181. 2006. View Article : Google Scholar

30 

Gaudiano G, Koch TH, LoBello M, et al: Lack of glutathione conjugation to adriamycin in human breast cancer MCF-7/DOX cells. Inhibition of glutathione S-transferase p1-1 by glutathione conjugates from anthracyclines. Biochem Pharmacol. 60:1915–1923. 2000. View Article : Google Scholar : PubMed/NCBI

31 

Ozkan A and Fiskin K: Protective effect of antioxidant enzymes against drug cytotoxicity in MCF-7 cells. Exp Oncol. 28:86–88. 2006.PubMed/NCBI

32 

Ilvasova D, Mixon G, Wang F, et al: Markers of oxidative status in a clinical model of oxidative assault: a pilot study in human blood following doxorubicin administration. Biomarkers. 14:321–325. 2009. View Article : Google Scholar : PubMed/NCBI

33 

Han XQ, Li ZH, Zhang JG, et al: Effect of decreased GSH on sensitivity of breast cancer cells to ADM. Sichuan Da Xue Xue Bao Yi Xue Ban. 36:770–774. 2007.(In Chinese).

34 

Vibet S, Goupille C, Bougnoux P, et al: Sensitization by docosahexaenoic acid (DHA) of breast cancer cells to anthracyclines through loss of glutathione peroxidase (GPx1) response. Free Radic Biol Med. 44:1483–1491. 2008. View Article : Google Scholar : PubMed/NCBI

35 

Sun W, Kalen AL, Smith BJ, et al: Enhancing the antitumor activity of adriamycin and ionizing radiation. Cancer Res. 69:4294–4300. 2009. View Article : Google Scholar : PubMed/NCBI

36 

Di X, Shiu RP, Newsham IF and Gewirtz DA: Apoptosis, autophagy, accelerated senescence and reactive oxygen in the response of human breast tumor cells to adriamycin. Biochem Pharmacol. 77:1139–1150. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Jardim BV, Moschetta MG, Leonel C, Gelaleti GB, Regiani VR, Ferreira LC, Lopes JR and de Campos Zuccari DA: Glutathione and glutathione peroxidase expression in breast cancer: An immunohistochemical and molecular study. Oncol Rep 30: 1119-1128, 2013.
APA
Jardim, B.V., Moschetta, M.G., Leonel, C., Gelaleti, G.B., Regiani, V.R., Ferreira, L.C. ... de Campos Zuccari, D.A. (2013). Glutathione and glutathione peroxidase expression in breast cancer: An immunohistochemical and molecular study. Oncology Reports, 30, 1119-1128. https://doi.org/10.3892/or.2013.2540
MLA
Jardim, B. V., Moschetta, M. G., Leonel, C., Gelaleti, G. B., Regiani, V. R., Ferreira, L. C., Lopes, J. R., de Campos Zuccari, D. A."Glutathione and glutathione peroxidase expression in breast cancer: An immunohistochemical and molecular study". Oncology Reports 30.3 (2013): 1119-1128.
Chicago
Jardim, B. V., Moschetta, M. G., Leonel, C., Gelaleti, G. B., Regiani, V. R., Ferreira, L. C., Lopes, J. R., de Campos Zuccari, D. A."Glutathione and glutathione peroxidase expression in breast cancer: An immunohistochemical and molecular study". Oncology Reports 30, no. 3 (2013): 1119-1128. https://doi.org/10.3892/or.2013.2540
Copy and paste a formatted citation
x
Spandidos Publications style
Jardim BV, Moschetta MG, Leonel C, Gelaleti GB, Regiani VR, Ferreira LC, Lopes JR and de Campos Zuccari DA: Glutathione and glutathione peroxidase expression in breast cancer: An immunohistochemical and molecular study. Oncol Rep 30: 1119-1128, 2013.
APA
Jardim, B.V., Moschetta, M.G., Leonel, C., Gelaleti, G.B., Regiani, V.R., Ferreira, L.C. ... de Campos Zuccari, D.A. (2013). Glutathione and glutathione peroxidase expression in breast cancer: An immunohistochemical and molecular study. Oncology Reports, 30, 1119-1128. https://doi.org/10.3892/or.2013.2540
MLA
Jardim, B. V., Moschetta, M. G., Leonel, C., Gelaleti, G. B., Regiani, V. R., Ferreira, L. C., Lopes, J. R., de Campos Zuccari, D. A."Glutathione and glutathione peroxidase expression in breast cancer: An immunohistochemical and molecular study". Oncology Reports 30.3 (2013): 1119-1128.
Chicago
Jardim, B. V., Moschetta, M. G., Leonel, C., Gelaleti, G. B., Regiani, V. R., Ferreira, L. C., Lopes, J. R., de Campos Zuccari, D. A."Glutathione and glutathione peroxidase expression in breast cancer: An immunohistochemical and molecular study". Oncology Reports 30, no. 3 (2013): 1119-1128. https://doi.org/10.3892/or.2013.2540
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