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Article

Overexpression of human telomerase reverse transcriptase promotes the motility and invasiveness of HepG2 cells in vitro

  • Authors:
    • Peng-Cheng Chen
    • Ji-Run Peng
    • Lei Huang
    • Wen-Xia Li
    • Wen-Zhen  Wang
    • Zhu-Qing-Qing Cui
    • Hui Han
    • Lei Gong
    • Da-Peng Xiang
    • Shi-Shi  Qiao
    • Xin Yu
    • Yu-Hua Wei
    • Li-Ping Ma
    • Na Li
    • Ji-Ye Zhu
    • Xi-Sheng  Leng
  • View Affiliations / Copyright

    Affiliations: Peking University People's Hospital, Beijing 100044, P.R. China
  • Pages: 1157-1164
    |
    Published online on: June 25, 2013
       https://doi.org/10.3892/or.2013.2563
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Abstract

Recent studies have indicated that telomerase activity promotes cancer invasion and metastasis, but the underlying mechanism remains unclear. Several studies have shown that expression of exogenous human telomerase reverse transcriptase (hTERT) can promote motility and invasiveness among telomerase-negative tumor cells, and inhibition of endogenous telomerase activity can reduce invasiveness in tumor cells. However, whether overexpression of hTERT can further enhance the motility and invasiveness of telomerase‑positive tumor cells has yet to be determined. In the present study, we showed that stable overexpression of hTERT can increase telomerase activity and telomere length, which significantly promotes the invasive and metastatic potential of telomerase‑positive HepG2 cells but does not affect cell proliferation. Further analysis suggested that enhanced invasiveness and metastasis may act through corresponding upregulation of mRNA and protein expression of matrix metalloproteinase 9 (MMP9) and Ras homolog gene family member C (RhoC). Our study indicated that exogenous expression of hTERT may promote invasiveness and metastasis through upregulation of MMP9 and RhoC.
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Copy and paste a formatted citation
Spandidos Publications style
Chen P, Peng J, Huang L, Li W, Wang W, Cui Z, Han H, Gong L, Xiang D, Qiao S, Qiao S, et al: Overexpression of human telomerase reverse transcriptase promotes the motility and invasiveness of HepG2 cells in vitro. Oncol Rep 30: 1157-1164, 2013.
APA
Chen, P., Peng, J., Huang, L., Li, W., Wang, W., Cui, Z. ... Leng, X. (2013). Overexpression of human telomerase reverse transcriptase promotes the motility and invasiveness of HepG2 cells in vitro. Oncology Reports, 30, 1157-1164. https://doi.org/10.3892/or.2013.2563
MLA
Chen, P., Peng, J., Huang, L., Li, W., Wang, W., Cui, Z., Han, H., Gong, L., Xiang, D., Qiao, S., Yu, X., Wei, Y., Ma, L., Li, N., Zhu, J., Leng, X."Overexpression of human telomerase reverse transcriptase promotes the motility and invasiveness of HepG2 cells in vitro". Oncology Reports 30.3 (2013): 1157-1164.
Chicago
Chen, P., Peng, J., Huang, L., Li, W., Wang, W., Cui, Z., Han, H., Gong, L., Xiang, D., Qiao, S., Yu, X., Wei, Y., Ma, L., Li, N., Zhu, J., Leng, X."Overexpression of human telomerase reverse transcriptase promotes the motility and invasiveness of HepG2 cells in vitro". Oncology Reports 30, no. 3 (2013): 1157-1164. https://doi.org/10.3892/or.2013.2563
Copy and paste a formatted citation
x
Spandidos Publications style
Chen P, Peng J, Huang L, Li W, Wang W, Cui Z, Han H, Gong L, Xiang D, Qiao S, Qiao S, et al: Overexpression of human telomerase reverse transcriptase promotes the motility and invasiveness of HepG2 cells in vitro. Oncol Rep 30: 1157-1164, 2013.
APA
Chen, P., Peng, J., Huang, L., Li, W., Wang, W., Cui, Z. ... Leng, X. (2013). Overexpression of human telomerase reverse transcriptase promotes the motility and invasiveness of HepG2 cells in vitro. Oncology Reports, 30, 1157-1164. https://doi.org/10.3892/or.2013.2563
MLA
Chen, P., Peng, J., Huang, L., Li, W., Wang, W., Cui, Z., Han, H., Gong, L., Xiang, D., Qiao, S., Yu, X., Wei, Y., Ma, L., Li, N., Zhu, J., Leng, X."Overexpression of human telomerase reverse transcriptase promotes the motility and invasiveness of HepG2 cells in vitro". Oncology Reports 30.3 (2013): 1157-1164.
Chicago
Chen, P., Peng, J., Huang, L., Li, W., Wang, W., Cui, Z., Han, H., Gong, L., Xiang, D., Qiao, S., Yu, X., Wei, Y., Ma, L., Li, N., Zhu, J., Leng, X."Overexpression of human telomerase reverse transcriptase promotes the motility and invasiveness of HepG2 cells in vitro". Oncology Reports 30, no. 3 (2013): 1157-1164. https://doi.org/10.3892/or.2013.2563
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