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Article

Human erythropoietin increases the pro-angiogenic potential of A2780 ovarian adenocarcinoma cells under hypoxic conditions

  • Authors:
    • Ján Kriška
    • Peter Solár
    • Lenka Varinská
    • Zuzana Solárová
    • Patrícia Kimáková
    • Ján Mojžiš
    • Peter Fedoročko
    • Arthur J. Sytkowski
  • View Affiliations / Copyright

    Affiliations: Institute of Biology and Ecology, Faculty of Science, P.J. Šafárik University, Košice, Slovak Republic, Department of Pharmacology, Faculty of Medicine, P.J. Šafárik University, Košice, Slovak Republic, Geriatric Nursing Clinic, Faculty of Medicine, P.J. Šafárik University, Košice, Slovak Republic, Oncology Therapeutic Area, Therapeutic Delivery Unit, Quintiles Transnational, Rockville, MD, USA
  • Pages: 1455-1462
    |
    Published online on: June 26, 2013
       https://doi.org/10.3892/or.2013.2566
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Abstract

Erythropoietin (Epo) is a key regulator of erythroid cell proliferation, differentiation and apoptosis. In the form of the recombinant protein, it is widely used to treat various types of anemias, including that associated with cancer and with the myelosuppressive effects of chemotherapy, particularly platinum-based regimens. Our previous studies confirmed the presence of Epo receptors (EpoRs) in ovarian adenocarcinoma cell lines and demonstrated that long-term Epo treatment of A2780 cells resulted in the development of a phenotype exhibiting both enhanced Epo signaling and increased paclitaxel resistance. In the present study, we carried out a series of experiments to analyze the pro-angiogenic potential of Epo-treated A2780 and SKOV-3 cells. Our studies revealed that conditioned media of Epo-treated A2780 cells had a stimulative effect on human umbilical vein endothelial cells (HUVECs). This effect was only seen when A2780 cells were incubated under hypoxic conditions. Furthermore, Epo increased the secretion of interleukin (IL)-4, IL-5, IL-6, IL-8, IL-10, IL-12, IL-13, GM-CSF and interferon-γ by A2780 cells that grew in hypoxic conditions. In this regard, conditioned media of hypoxic and Epo-treated A2780 cells induced a significant phosphorylation of STAT-5 in HUVECs. Our results may have important implications for ovarian cancer patients receiving Epo.
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Copy and paste a formatted citation
Spandidos Publications style
Kriška J, Solár P, Varinská L, Solárová Z, Kimáková P, Mojžiš J, Fedoročko P and Sytkowski AJ: Human erythropoietin increases the pro-angiogenic potential of A2780 ovarian adenocarcinoma cells under hypoxic conditions. Oncol Rep 30: 1455-1462, 2013.
APA
Kriška, J., Solár, P., Varinská, L., Solárová, Z., Kimáková, P., Mojžiš, J. ... Sytkowski, A.J. (2013). Human erythropoietin increases the pro-angiogenic potential of A2780 ovarian adenocarcinoma cells under hypoxic conditions. Oncology Reports, 30, 1455-1462. https://doi.org/10.3892/or.2013.2566
MLA
Kriška, J., Solár, P., Varinská, L., Solárová, Z., Kimáková, P., Mojžiš, J., Fedoročko, P., Sytkowski, A. J."Human erythropoietin increases the pro-angiogenic potential of A2780 ovarian adenocarcinoma cells under hypoxic conditions". Oncology Reports 30.3 (2013): 1455-1462.
Chicago
Kriška, J., Solár, P., Varinská, L., Solárová, Z., Kimáková, P., Mojžiš, J., Fedoročko, P., Sytkowski, A. J."Human erythropoietin increases the pro-angiogenic potential of A2780 ovarian adenocarcinoma cells under hypoxic conditions". Oncology Reports 30, no. 3 (2013): 1455-1462. https://doi.org/10.3892/or.2013.2566
Copy and paste a formatted citation
x
Spandidos Publications style
Kriška J, Solár P, Varinská L, Solárová Z, Kimáková P, Mojžiš J, Fedoročko P and Sytkowski AJ: Human erythropoietin increases the pro-angiogenic potential of A2780 ovarian adenocarcinoma cells under hypoxic conditions. Oncol Rep 30: 1455-1462, 2013.
APA
Kriška, J., Solár, P., Varinská, L., Solárová, Z., Kimáková, P., Mojžiš, J. ... Sytkowski, A.J. (2013). Human erythropoietin increases the pro-angiogenic potential of A2780 ovarian adenocarcinoma cells under hypoxic conditions. Oncology Reports, 30, 1455-1462. https://doi.org/10.3892/or.2013.2566
MLA
Kriška, J., Solár, P., Varinská, L., Solárová, Z., Kimáková, P., Mojžiš, J., Fedoročko, P., Sytkowski, A. J."Human erythropoietin increases the pro-angiogenic potential of A2780 ovarian adenocarcinoma cells under hypoxic conditions". Oncology Reports 30.3 (2013): 1455-1462.
Chicago
Kriška, J., Solár, P., Varinská, L., Solárová, Z., Kimáková, P., Mojžiš, J., Fedoročko, P., Sytkowski, A. J."Human erythropoietin increases the pro-angiogenic potential of A2780 ovarian adenocarcinoma cells under hypoxic conditions". Oncology Reports 30, no. 3 (2013): 1455-1462. https://doi.org/10.3892/or.2013.2566
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