MicroRNA-128 promotes cell-cell adhesion in U87 glioma cells via regulation of EphB2

Lin,Lina; Chen,Xulin; Peng,Xuelin; Zhou,Jianwen; Kung,Hsiang-Fu; Lin,Marie C.; Jiang,Songshan

doi:10.3892/or.2013.2596

MicroRNA-128 promotes cell-cell adhesion in U87 glioma cells via regulation of EphB2

Authors:
- Lina Lin
- Xulin Chen
- Xuelin Peng
- Jianwen Zhou
- Hsiang-Fu Kung
- Marie C. Lin
- Songshan Jiang
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Affiliations: Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, P.R. China, School of Biomedical Science and Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, Hong Kong SAR, P.R. China, Brain Tumor Centre, Division of Neurosurgery, Department of Surgery, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, P.R. China
Published online on: July 5, 2013 https://doi.org/10.3892/or.2013.2596
Pages: 1239-1248

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Abstract

MicroRNAs (miRNAs) are small, non-coding RNAs which regulate gene expression at the post-transcriptional level. Abnormal expression of miRNAs occurs frequently in human tumors. Despite the fact that reduced expression of miR-128 has been observed in glioma tissues and cells, the role of miR-128 in tumors has not been fully characterized. In the present study, cell adhesion assays indicated that overexpression of miR-128 can promote cell-cell adhesion. Target site prediction algorithms indicated that miR-128 binds the 3'-untranslated regions of erythropoietin-producing hepatocellular receptor (Eph)B1 and EphB2 mRNAs. Luciferase reporter assays confirmed that miR-128 binds and regulates EphB1 and EphB2 mRNAs. Overexpression of EphB2 reduced the ability of miR-128 to promote cell-cell adhesion. The wound-healing assay indicated that miR-128 significantly inhibited cell migration via EphB2. This study revealed the novel functions of miR-128 in cell-cell adhesion and cell migration in glioma cells through the regulation of EphB2, and identified EphB1 and EphB2 as novel miR-128 targets.

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