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Article

Overexpression of ERα inhibits proliferation and invasion of MKN28 gastric cancer cells by suppressing β-catenin

  • Authors:
    • Jichun Zhou
    • Rongyue Teng
    • Chaoyang Xu
    • Qinchuan Wang
    • Jufeng  Guo
    • Chenpu Xu
    • Ziduo Li
    • Shuduo Xie
    • Jianguo Shen
    • Linbo Wang
  • View Affiliations / Copyright

    Affiliations: Department of Surgical Oncology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310016, P.R. China, Department of Breast and Thyroid Surgery, Shaoxing People's Hospital, The First Affiliated Hospital of Shaoxing Liberal Art College, Shaoxing, Zhejiang 312000, P.R. China
  • Pages: 1622-1630
    |
    Published online on: July 9, 2013
       https://doi.org/10.3892/or.2013.2610
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Abstract

The relationship between estrogen receptor (ER)α and patient prognosis has been identified in gastric cancer; however, the definite role of ERα in gastric cancer remains to be fully elucidated. The aim of the present in vitro study was to investigate the impact of ERα on cell proliferation, migration and invasion in gastric cancer cell lines. We investigated the biological effect of ERα overexpression on gastric carcinoma cells. An MKN28 gastric cancer cell line stably overexpressing ERα was established. The effect of ERα overexpression on cell growth was assessed by evaluating cell survival, colony formation, cell cycle progression and apoptosis. Cell migration and invasion were detected by Transwell migration/invasion assays. The protein levels of several potentially involved genes were determined by western blotting to elucidate the underlying molecular mechanisms. The Student's t-test was used to determine the statistical differences between various experimental and control groups, and one-way ANOVA test was used to determine the difference between three or more groups. The results showed that ERα overexpression significantly inhibited cell growth and proliferation, blocked cell entry into the G1/G0 phase and promoted cell apoptosis. In addition, ERα reduced the motility and invasion of gastric cancer cells. These phenotypes may partly be explained by a decrease in β-catenin expression caused by ERα overexpression. ERα overexpression effectively inhibited cell growth and cancer progression by suppressing β-catenin in gastric cancer, identifying ERα as a promising target with therapeutic potential for development of new approaches to treat gastric cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Zhou J, Teng R, Xu C, Wang Q, Guo J, Xu C, Li Z, Xie S, Shen J, Wang L, Wang L, et al: Overexpression of ERα inhibits proliferation and invasion of MKN28 gastric cancer cells by suppressing β-catenin. Oncol Rep 30: 1622-1630, 2013.
APA
Zhou, J., Teng, R., Xu, C., Wang, Q., Guo, J., Xu, C. ... Wang, L. (2013). Overexpression of ERα inhibits proliferation and invasion of MKN28 gastric cancer cells by suppressing β-catenin. Oncology Reports, 30, 1622-1630. https://doi.org/10.3892/or.2013.2610
MLA
Zhou, J., Teng, R., Xu, C., Wang, Q., Guo, J., Xu, C., Li, Z., Xie, S., Shen, J., Wang, L."Overexpression of ERα inhibits proliferation and invasion of MKN28 gastric cancer cells by suppressing β-catenin". Oncology Reports 30.4 (2013): 1622-1630.
Chicago
Zhou, J., Teng, R., Xu, C., Wang, Q., Guo, J., Xu, C., Li, Z., Xie, S., Shen, J., Wang, L."Overexpression of ERα inhibits proliferation and invasion of MKN28 gastric cancer cells by suppressing β-catenin". Oncology Reports 30, no. 4 (2013): 1622-1630. https://doi.org/10.3892/or.2013.2610
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou J, Teng R, Xu C, Wang Q, Guo J, Xu C, Li Z, Xie S, Shen J, Wang L, Wang L, et al: Overexpression of ERα inhibits proliferation and invasion of MKN28 gastric cancer cells by suppressing β-catenin. Oncol Rep 30: 1622-1630, 2013.
APA
Zhou, J., Teng, R., Xu, C., Wang, Q., Guo, J., Xu, C. ... Wang, L. (2013). Overexpression of ERα inhibits proliferation and invasion of MKN28 gastric cancer cells by suppressing β-catenin. Oncology Reports, 30, 1622-1630. https://doi.org/10.3892/or.2013.2610
MLA
Zhou, J., Teng, R., Xu, C., Wang, Q., Guo, J., Xu, C., Li, Z., Xie, S., Shen, J., Wang, L."Overexpression of ERα inhibits proliferation and invasion of MKN28 gastric cancer cells by suppressing β-catenin". Oncology Reports 30.4 (2013): 1622-1630.
Chicago
Zhou, J., Teng, R., Xu, C., Wang, Q., Guo, J., Xu, C., Li, Z., Xie, S., Shen, J., Wang, L."Overexpression of ERα inhibits proliferation and invasion of MKN28 gastric cancer cells by suppressing β-catenin". Oncology Reports 30, no. 4 (2013): 1622-1630. https://doi.org/10.3892/or.2013.2610
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