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October 2013 Volume 30 Issue 4

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International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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Article

Exome capture sequencing reveals new insights into hepatitis B virus-induced hepatocellular carcinoma at the early stage of tumorigenesis

  • Authors:
    • Yong Chen
    • Lijuan Wang
    • Hexiang Xu
    • Xingxiang Liu
    • Yingren Zhao
  • View Affiliations / Copyright

    Affiliations: Institute of Liver Diseases, Huai'an Fourth People's Hospital, Huai'an, Jiangsu 223002, P.R. China, Department of Infectious Diseases, The First Affiliated Hospital of the Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China
  • Pages: 1906-1912
    |
    Published online on: August 2, 2013
       https://doi.org/10.3892/or.2013.2652
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Abstract

Hepatocellular carcinoma (HCC), the most common type of liver cancer, is the third primary cause of cancer-related mortality worldwide. The molecular mechanisms underlying the initiation and formation of HCC remain obscure. In the present study, we performed exome sequencing using tumor and normal tissues from 3 hepatitis B virus (HBV)-positive BCLC stage A HCC patients. Bioinformatic analysis was performed to find candidate protein-altering somatic mutations. Eighty damaging mutations were validated and 59 genes were reported to be mutated in HBV-related HCCs for the first time here. Further analysis using whole genome sequencing (WGS) data of 88 HBV-related HCC patients from the European Genome-phenome Archive database showed that mutations in 33 of the 59 genes were also detected in other samples. Variants of two newly found genes, ZNF717 and PARP4, were detected in more than 10% of the WGS samples. Several other genes, such as FLNA and CNTN2, are also noteworthy. Thus, the exome sequencing analysis of three BCLC stage A patients provides new insights into the molecular events governing the early steps of HBV-induced HCC tumorigenesis.
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Copy and paste a formatted citation
Spandidos Publications style
Chen Y, Wang L, Xu H, Liu X and Zhao Y: Exome capture sequencing reveals new insights into hepatitis B virus-induced hepatocellular carcinoma at the early stage of tumorigenesis. Oncol Rep 30: 1906-1912, 2013.
APA
Chen, Y., Wang, L., Xu, H., Liu, X., & Zhao, Y. (2013). Exome capture sequencing reveals new insights into hepatitis B virus-induced hepatocellular carcinoma at the early stage of tumorigenesis. Oncology Reports, 30, 1906-1912. https://doi.org/10.3892/or.2013.2652
MLA
Chen, Y., Wang, L., Xu, H., Liu, X., Zhao, Y."Exome capture sequencing reveals new insights into hepatitis B virus-induced hepatocellular carcinoma at the early stage of tumorigenesis". Oncology Reports 30.4 (2013): 1906-1912.
Chicago
Chen, Y., Wang, L., Xu, H., Liu, X., Zhao, Y."Exome capture sequencing reveals new insights into hepatitis B virus-induced hepatocellular carcinoma at the early stage of tumorigenesis". Oncology Reports 30, no. 4 (2013): 1906-1912. https://doi.org/10.3892/or.2013.2652
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Y, Wang L, Xu H, Liu X and Zhao Y: Exome capture sequencing reveals new insights into hepatitis B virus-induced hepatocellular carcinoma at the early stage of tumorigenesis. Oncol Rep 30: 1906-1912, 2013.
APA
Chen, Y., Wang, L., Xu, H., Liu, X., & Zhao, Y. (2013). Exome capture sequencing reveals new insights into hepatitis B virus-induced hepatocellular carcinoma at the early stage of tumorigenesis. Oncology Reports, 30, 1906-1912. https://doi.org/10.3892/or.2013.2652
MLA
Chen, Y., Wang, L., Xu, H., Liu, X., Zhao, Y."Exome capture sequencing reveals new insights into hepatitis B virus-induced hepatocellular carcinoma at the early stage of tumorigenesis". Oncology Reports 30.4 (2013): 1906-1912.
Chicago
Chen, Y., Wang, L., Xu, H., Liu, X., Zhao, Y."Exome capture sequencing reveals new insights into hepatitis B virus-induced hepatocellular carcinoma at the early stage of tumorigenesis". Oncology Reports 30, no. 4 (2013): 1906-1912. https://doi.org/10.3892/or.2013.2652
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