Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1021-335X Online ISSN: 1791-2431
Journal Cover
October 2013 Volume 30 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
October 2013 Volume 30 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Heparin-binding EGF-like growth factor enhances the activity of invasion and metastasis in thyroid cancer cells

  • Authors:
    • Ichiro Ota
    • Shigeki Higashiyama
    • Takashi Masui
    • Katsunari Yane
    • Hiroshi Hosoi
    • Nariaki Matsuura
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology-Head and Neck Surgery, Nara Medical University, Kashihara, Nara, Japan, Department of Biochemistry and Molecular Genetics, Ehime University Graduate School of Medicine, Toon, Ehime, Japan, Department of Otolaryngology, Kinki University Nara Hospital, Ikoma, Nara, Japan, Department of Molecular Pathology, School of Allied Health Science, Osaka University Faculty of Medicine, Suita, Osaka, Japan
    Copyright: © Ota et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 1593-1600
    |
    Published online on: August 6, 2013
       https://doi.org/10.3892/or.2013.2659
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Thyroid cancer sometimes contains poorly differentiated components, which have the potential of invasion and metastasis. We evaluated the possible roles of heparin-binding EGF-like growth factor (HB-EGF), a member of the epidermal growth factor (EGF) family, in cell growth and invasion of thyroid cancer cells, and demonstrated that HB-EGF is not only a potent mitogen but also a chemotactic factor in the thyroid cancer cells 8305C and SW579. The HB-EGF-mediated chemotaxis was inhibited by neutralizing antibody against the EGF receptor (EGFR/HER1/ErbB1) or tyrphostin AG1478, a specific inhibitor of the EGFR tyrosine kinase. The HB-EGF mRNA and protein expression was also analyzed using RT-PCR and immunofluorescence methods, respectively. In addition, in clinical immunohistochemical study, increased expression of HB-EGF and its receptors, HER1 and EGFR4 (HER4/ErbB4), was observed in thyroid carcinoma cells. Our findings suggest that HB-EGF acts as a potent paracrine and/or autocrine chemotactic factor as well as a mitogen that mediates HER1 and/or HER4 in the invasion and metastasis of thyroid carcinoma cells, including poorly differentiated papillary carcinomas or undifferentiated/anaplastic carcinomas. These data may aid in the development of novel therapeutic strategies for thyroid cancer.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Ito Y, Hirokawa M, Kihara M, et al: Prognostic value of poorly differentiated carcinoma in Japanese Society of Thyroid Surgery in a series of papillary thyroid carcinoma patients: comparison with risk classification system in Kuma Hospital. Endocr J. 59:817–821. 2012. View Article : Google Scholar

2 

Xing M: Genetic alterations in the phosphatidylinositol-3 kinase/Akt pathway in thyroid cancer. Thyroid. 20:697–706. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Stoscheck CM and King LE Jr: Role of epidermal growth factor in carcinogenesis. Cancer Res. 46:1030–1037. 1986.PubMed/NCBI

4 

Higashiyama S, Abraham JA, Miller J, Fiddes JC and Klagsbrun M: A heparin-binding growth factor secreted by macrophage-like cells that is related to EGF. Science. 251:936–939. 1991. View Article : Google Scholar : PubMed/NCBI

5 

Raab G, Higashiyama S, Hetelekidis S, et al: Biosynthesis and processing by phorbol ester of the cells surface-associated precursor form of heparin-binding EGF-like growth factor. Biochem Biophys Res Commun. 204:592–597. 1994. View Article : Google Scholar : PubMed/NCBI

6 

Besner G, Higashiyama S and Klagsbrun M: Isolation and characterization of a macrophage-derived heparin-binding growth factor. Cell Regul. 1:811–819. 1990.PubMed/NCBI

7 

Higashiyama S, Lau K, Besner GE, Abraham JA and Klagsbrun M: Structure of heparin-binding EGF-like growth factor. Multiple forms, primary structure, and glycosylation of the mature protein. J Biol Chem. 267:6205–6212. 1992.PubMed/NCBI

8 

Higashiyama S, Abraham JA and Klagsbrun M: Heparin-binding EGF-like growth factor stimulation of smooth muscle cell migration: dependence on interactions with cell surface heparan sulfate. J Cell Biol. 122:933–940. 1993. View Article : Google Scholar : PubMed/NCBI

9 

Marikovsky M, Breuing K, Liu PY, et al: Appearance of heparin-binding EGF-like growth factor in wound fluid as a response to injury. Proc Natl Acad Sci USA. 90:3889–3893. 1993. View Article : Google Scholar : PubMed/NCBI

10 

Hashimoto K, Higashiyama S, Asada H, et al: Heparin-binding epidermal growth factor-like growth factor is an autocrine growth factor for human keratinocytes. J Biol Chem. 269:20060–20066. 1994.PubMed/NCBI

11 

Peoples GE, Blotnick S, Takahashi K, Freeman MR, Klagsbrun M and Eberlein TJ: T lymphocytes that infiltrate tumors and atherosclerotic plaques produce heparin-binding epidermal growth factor-like growth factor and basic fibroblast growth factor: a potential pathologic role. Proc Natl Acad Sci USA. 92:6547–6551. 1995. View Article : Google Scholar

12 

Miyagawa J, Higashiyama S, Kawata S, et al: Localization of heparin-binding EGF-like growth factor in the smooth muscle cells and macrophages of human atherosclerotic plaques. J Clin Invest. 95:404–411. 1995. View Article : Google Scholar : PubMed/NCBI

13 

Powell PP, Klagsbrun M, Abraham JA and Jones RC: Eosinophils expressing heparin-binding EGF-like growth factor mRNA localize around lung microvessels in pulmonary hypertension. Am J Pathol. 143:784–793. 1993.PubMed/NCBI

14 

Mangrulkar RS, Ono M, Ishikawa M, Takashima S, Klagsbrun M and Nowak RA: Isolation and characterization of heparin-binding growth factors in human leiomyomas and normal myometrium. Biol Reprod. 53:636–646. 1995. View Article : Google Scholar : PubMed/NCBI

15 

Higashiyama S, Iwamoto R, Goishi K, et al: The membrane protein CD9/DRAP 27 potentiates the juxtacrine growth factor activity of the membrane-anchored heparin-binding EGF-like growth factor. J Cell Biol. 128:929–938. 1995. View Article : Google Scholar : PubMed/NCBI

16 

Iwamoto R, Higashiyama S, Mitamura T, Taniguchi N, Klagsbrun M and Mekada E: Heparin-binding EGF-like growth factor, which acts as the diphtheria toxin receptor, forms a complex with membrane protein DRAP27/CD9, which up-regulates functional receptors and diphtheria toxin sensitivity. EMBO J. 13:2322–2330. 1994.

17 

Yotsumoto F, Yagi H, Suzuki SO, et al: Validation of HB-EGF and amphiregulin as targets for human cancer therapy. Biochem Biophys Res Commun. 365:555–561. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Miyamoto S, Hirata M, Yamazaki A, et al: Heparin-binding EGF-like growth factor is a promising target for ovarian cancer therapy. Cancer Res. 64:5720–5727. 2004. View Article : Google Scholar : PubMed/NCBI

19 

Inui Y, Higashiyama S, Kawata S, et al: Expression of heparin-binding epidermal growth factor in human hepatocellular carcinoma. Gastroenterology. 107:1799–1804. 1994.PubMed/NCBI

20 

Naef M, Yokoyama M, Friess H, Buchler MW and Korc M: Co-expression of heparin-binding EGF-like growth factor and related peptides in human gastric carcinoma. Int J Cancer. 66:315–321. 1996. View Article : Google Scholar : PubMed/NCBI

21 

Kobrin MS, Funatomi H, Friess H, Buchler MW, Stathis P and Korc M: Induction and expression of heparin-binding EGF-like growth factor in human pancreatic cancer. Biochem Biophys Res Commun. 202:1705–1709. 1994. View Article : Google Scholar : PubMed/NCBI

22 

Ohnishi Y, Inoue H, Furukawa M, Kakudo K and Nozaki M: Heparin-binding epidermal growth factor-like growth factor is a potent regulator of invasion activity in oral squamous cell carcinoma. Oncol Rep. 27:954–958. 2012.PubMed/NCBI

23 

Haugen DR, Akslen LA, Varhaug JE and Lillehaug JR: Expression of c-erbB-3 and c-erbB-4 proteins in papillary thyroid carcinomas. Cancer Res. 56:1184–1188. 1996.PubMed/NCBI

24 

Elenius K, Paul S, Allison G, Sun J and Klagsbrun M: Activation of HER4 by heparin-binding EGF-like growth factor stimulates chemotaxis but not proliferation. EMBO J. 16:1268–1278. 1997. View Article : Google Scholar : PubMed/NCBI

25 

Ito T, Seyama T, Mizuno T, et al: Unique association of p53 mutations with undifferentiated but not with differentiated carcinomas of the thyroid gland. Cancer Res. 52:1369–1371. 1992.PubMed/NCBI

26 

Fogh J, Wright WC and Loveless JD: Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst. 58:209–214. 1977.PubMed/NCBI

27 

Sobin LH and Wittekind C: International Union against Cancer. TNM Classification of Malignant Tumours. 6th edition. Wiley-Liss; New York, NY: 2002

28 

Ishiyama M, Miyazono Y, Sasamoto K, Ohkura Y and Ueno K: A highly water-soluble disulfonated tetrazolium salt as a chromogenic indicator for NADH as well as cell viability. Talanta. 44:1299–1305. 1997. View Article : Google Scholar : PubMed/NCBI

29 

Boyden S: The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes. J Exp Med. 115:453–466. 1962. View Article : Google Scholar : PubMed/NCBI

30 

Yoshida A, Anand-Apte B and Zetter BR: Differential endothelial migration and proliferation to basic fibroblast growth factor and vascular endothelial growth factor. Growth Factors. 13:57–64. 1996. View Article : Google Scholar

31 

Goodman SL, Vollmers HP and Birchmeier W: Control of cell locomotion: perturbation with an antibody directed against specific glycoproteins. Cell. 41:1029–1038. 1985. View Article : Google Scholar : PubMed/NCBI

32 

Mannino RJ, Ballmer K, Zeltner D and Burger MM: An inhibitor of animal cell growth increases cell-to-cell adhesion. J Cell Biol. 91:855–859. 1981. View Article : Google Scholar : PubMed/NCBI

33 

Giancotti FG and Ruoslahti E: Elevated levels of the α5β1fibronectin receptor suppress the transformed phenotype of Chinese hamster ovary cells. Cell. 60:849–859. 1990.

34 

Chomczynski P and Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 162:156–159. 1987. View Article : Google Scholar : PubMed/NCBI

35 

Datta YH, Adams PT, Drobyski WR, Ethier SP, Terry VH and Roth MS: Sensitive detection of occult breast cancer by the reverse-transcriptase polymerase chain reaction. J Clin Oncol. 12:475–482. 1994.

36 

Noguchi S, Aihara T, Nakamori S, et al: The detection of breast carcinoma micrometastases in axillary lymph nodes by means of reverse transcriptase-polymerase chain reaction. Cancer. 74:1595–1600. 1994. View Article : Google Scholar

37 

Haugen DR, Akslen LA, Varhaug JE and Lillehaug JR: Demonstration of a TGF-alpha-EGF-receptor autocrine loop and c-myc protein over-expression in papillary thyroid carcinomas. Int J Cancer. 55:37–43. 1993. View Article : Google Scholar : PubMed/NCBI

38 

Gorgoulis V, Aninos D, Priftis C, et al: Expression of epidermal growth factor, transforming growth factor-alpha and epidermal growth factor receptor in thyroid tumors. In Vivo. 6:291–296. 1992.PubMed/NCBI

39 

Aasland R, Akslen LA, Varhaug JE and Lillehaug JR: Co-expression of the genes encoding transforming growth factor-alpha and its receptor in papillary carcinomas of the thyroid. Int J Cancer. 46:382–387. 1990. View Article : Google Scholar : PubMed/NCBI

40 

Freeman MR, Paul S, Kaefer M, et al: Heparin-binding EGF-like growth factor in the human prostate: synthesis predominantly by interstitial and vascular smooth muscle cells and action as a carcinoma cell mitogen. J Cell Biochem. 68:328–338. 1998. View Article : Google Scholar : PubMed/NCBI

41 

Holting T, Siperstein AE, Clark OH and Duh QY: Epidermal growth factor (EGF)- and transforming growth factor alpha-stimulated invasion and growth of follicular thyroid cancer cells can be blocked by antagonism to the EGF receptor and tyrosine kinase in vitro. Eur J Endocrinol. 132:229–235. 1995. View Article : Google Scholar

42 

Ongusaha PP, Kwak JC, Zwible AJ, et al: HB-EGF is a potent inducer of tumor growth and angiogenesis. Cancer Res. 64:5283–5290. 2004. View Article : Google Scholar : PubMed/NCBI

43 

Miyoshi E, Higashiyama S, Nakagawa T, Hayashi N and Taniguchi N: Membrane-anchored heparin-binding epidermal growth factor-like growth factor acts as a tumor survival factor in a hepatoma cell line. J Biol Chem. 272:14349–14355. 1997. View Article : Google Scholar : PubMed/NCBI

44 

Shah JP, Loree TR, Dharker D, Strong EW, Begg C and Vlamis V: Prognostic factors in differentiated carcinoma of the thyroid gland. Am J Surg. 164:658–661. 1992. View Article : Google Scholar : PubMed/NCBI

45 

Suzuki M, Raab G, Moses MA, Fernandez CA and Klagsbrun M: Matrix metalloproteinase-3 releases active heparin-binding EGF-like growth factor by cleavage at a specific juxtamembrane site. J Biol Chem. 272:31730–31737. 1997. View Article : Google Scholar : PubMed/NCBI

46 

Izumi Y, Hirata M, Hasuwa H, et al: A metalloprotease-disintegrin, MDC9/meltrin-gamma/ADAM9 and PKCdelta are involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. EMBO J. 17:7260–7272. 1998. View Article : Google Scholar

47 

Koshikawa N, Mizushima H, Minegishi T, et al: Proteolytic activation of heparin-binding EGF-like growth factor by membrane-type matrix metalloproteinase-1 in ovarian carcinoma cells. Cancer Sci. 102:111–116. 2010. View Article : Google Scholar : PubMed/NCBI

48 

Nakamura H, Ueno H, Yamashita K, et al: Enhanced production and activation of progelatinase A mediated by membrane-type 1 matrix metalloproteinase in human papillary thyroid carcinomas. Cancer Res. 59:467–473. 1999.PubMed/NCBI

49 

Aust G, Hofmann A, Laue S, Rost A, Kohler T and Scherbaum WA: Human thyroid carcinoma cell lines and normal thyrocytes: expression and regulation of matrix metalloproteinase-1 and tissue matrix metalloproteinase inhibitor-1 messenger-RNA and protein. Thyroid. 7:713–724. 1997. View Article : Google Scholar

50 

Hofmann A, Laue S, Rost AK, Scherbaum WA and Aust G: mRNA levels of membrane-type 1 matrix metalloproteinase (MT1-MMP), MMP-2, and MMP-9 and of their inhibitors TIMP-2 and TIMP-3 in normal thyrocytes and thyroid carcinoma cell lines. Thyroid. 8:203–214. 1998. View Article : Google Scholar : PubMed/NCBI

51 

Higashiyama S, Iwabuki H, Morimoto C, Hieda M, Inoue H and Matsushita N: Membrane-anchored growth factors, the epidermal growth factor family: beyond receptor ligands. Cancer Sci. 99:214–220. 2008. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Ota I, Higashiyama S, Masui T, Yane K, Hosoi H and Matsuura N: Heparin-binding EGF-like growth factor enhances the activity of invasion and metastasis in thyroid cancer cells. Oncol Rep 30: 1593-1600, 2013.
APA
Ota, I., Higashiyama, S., Masui, T., Yane, K., Hosoi, H., & Matsuura, N. (2013). Heparin-binding EGF-like growth factor enhances the activity of invasion and metastasis in thyroid cancer cells. Oncology Reports, 30, 1593-1600. https://doi.org/10.3892/or.2013.2659
MLA
Ota, I., Higashiyama, S., Masui, T., Yane, K., Hosoi, H., Matsuura, N."Heparin-binding EGF-like growth factor enhances the activity of invasion and metastasis in thyroid cancer cells". Oncology Reports 30.4 (2013): 1593-1600.
Chicago
Ota, I., Higashiyama, S., Masui, T., Yane, K., Hosoi, H., Matsuura, N."Heparin-binding EGF-like growth factor enhances the activity of invasion and metastasis in thyroid cancer cells". Oncology Reports 30, no. 4 (2013): 1593-1600. https://doi.org/10.3892/or.2013.2659
Copy and paste a formatted citation
x
Spandidos Publications style
Ota I, Higashiyama S, Masui T, Yane K, Hosoi H and Matsuura N: Heparin-binding EGF-like growth factor enhances the activity of invasion and metastasis in thyroid cancer cells. Oncol Rep 30: 1593-1600, 2013.
APA
Ota, I., Higashiyama, S., Masui, T., Yane, K., Hosoi, H., & Matsuura, N. (2013). Heparin-binding EGF-like growth factor enhances the activity of invasion and metastasis in thyroid cancer cells. Oncology Reports, 30, 1593-1600. https://doi.org/10.3892/or.2013.2659
MLA
Ota, I., Higashiyama, S., Masui, T., Yane, K., Hosoi, H., Matsuura, N."Heparin-binding EGF-like growth factor enhances the activity of invasion and metastasis in thyroid cancer cells". Oncology Reports 30.4 (2013): 1593-1600.
Chicago
Ota, I., Higashiyama, S., Masui, T., Yane, K., Hosoi, H., Matsuura, N."Heparin-binding EGF-like growth factor enhances the activity of invasion and metastasis in thyroid cancer cells". Oncology Reports 30, no. 4 (2013): 1593-1600. https://doi.org/10.3892/or.2013.2659
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team