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Article

Promoter methylation status of the FHIT gene and Fhit expression: Association with HER2/neu status in breast cancer patients

  • Authors:
    • Young Ju Jeong
    • Hye Yeon Jeong
    • Sang Min Lee
    • Jin  Gu Bong
    • Sung Hwan Park
    • Hoon Kyu Oh
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, College of Medicine, Catholic University of Daegu, Daegu, Republic of Korea, Department of Pathology, College of Medicine, Catholic University of Daegu, Daegu, Republic of Korea
  • Pages: 2270-2278
    |
    Published online on: August 20, 2013
       https://doi.org/10.3892/or.2013.2668
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Abstract

Aberrant DNA methylation has been recognized to contribute to breast carcinogenesis, and promoter hypermethylation of several tumor suppressor genes has been correlated with decreased gene expression. The fragile histidine triad (FHIT) gene is a putative tumor suppressor gene in breast and other types of cancer, and loss of Fhit expression has been observed in breast cancer. The aim of the present study was to evaluate the association between methylation of the FHIT gene and its expression in breast cancer, and to investigate whether methylation and expression of the FHIT gene correlates with clinicopathological characteristics in relation to human epidermal growth factor receptor 2 (HER2) status. Pyrosequencing of bisulfite-treated DNA was performed to study the methylation status of the FHIT gene in 60 breast cancer samples. We examined the expression of Fhit using tissue microarrays by immunohistochemical staining. FHIT methylation was detected in 96.7% and the positive expression rate of Fhit was 87.3% of the patients. The mean methylation level of the FHIT gene was associated with intratumoral inflammation. Methylation level of the FHIT gene had no significant differences according to molecular subtypes. Loss of Fhit expression was associated with large tumor size, basal-like subtype and positive expression of EGFR. In HER2-negative breast cancer, loss of Fhit expression was significantly associated with tumor size, estrogen receptor status and Ki-67 proliferation index. No significant correlation between methylation of the FHIT gene and its expression was observed in the present study. Our results suggest that loss of Fhit expression in breast cancer is associated with poor prognostic features, and it is also relevant to the results in HER2-negative breast cancer. Further studies with larger sample sizes and longer follow-up are required to clarify the predictive and prognostic value of Fhit expression and the FHIT gene methylation status in breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Jeong YJ, Jeong HY, Lee SM, Bong JG, Park SH and Oh HK: Promoter methylation status of the FHIT gene and Fhit expression: Association with HER2/neu status in breast cancer patients. Oncol Rep 30: 2270-2278, 2013.
APA
Jeong, Y.J., Jeong, H.Y., Lee, S.M., Bong, J.G., Park, S.H., & Oh, H.K. (2013). Promoter methylation status of the FHIT gene and Fhit expression: Association with HER2/neu status in breast cancer patients. Oncology Reports, 30, 2270-2278. https://doi.org/10.3892/or.2013.2668
MLA
Jeong, Y. J., Jeong, H. Y., Lee, S. M., Bong, J. G., Park, S. H., Oh, H. K."Promoter methylation status of the FHIT gene and Fhit expression: Association with HER2/neu status in breast cancer patients". Oncology Reports 30.5 (2013): 2270-2278.
Chicago
Jeong, Y. J., Jeong, H. Y., Lee, S. M., Bong, J. G., Park, S. H., Oh, H. K."Promoter methylation status of the FHIT gene and Fhit expression: Association with HER2/neu status in breast cancer patients". Oncology Reports 30, no. 5 (2013): 2270-2278. https://doi.org/10.3892/or.2013.2668
Copy and paste a formatted citation
x
Spandidos Publications style
Jeong YJ, Jeong HY, Lee SM, Bong JG, Park SH and Oh HK: Promoter methylation status of the FHIT gene and Fhit expression: Association with HER2/neu status in breast cancer patients. Oncol Rep 30: 2270-2278, 2013.
APA
Jeong, Y.J., Jeong, H.Y., Lee, S.M., Bong, J.G., Park, S.H., & Oh, H.K. (2013). Promoter methylation status of the FHIT gene and Fhit expression: Association with HER2/neu status in breast cancer patients. Oncology Reports, 30, 2270-2278. https://doi.org/10.3892/or.2013.2668
MLA
Jeong, Y. J., Jeong, H. Y., Lee, S. M., Bong, J. G., Park, S. H., Oh, H. K."Promoter methylation status of the FHIT gene and Fhit expression: Association with HER2/neu status in breast cancer patients". Oncology Reports 30.5 (2013): 2270-2278.
Chicago
Jeong, Y. J., Jeong, H. Y., Lee, S. M., Bong, J. G., Park, S. H., Oh, H. K."Promoter methylation status of the FHIT gene and Fhit expression: Association with HER2/neu status in breast cancer patients". Oncology Reports 30, no. 5 (2013): 2270-2278. https://doi.org/10.3892/or.2013.2668
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