Expression of the Arp2/3 complex in human gliomas and its role in the migration and invasion of glioma cells

  • Authors:
    • Zhifeng Liu
    • Xuejun Yang
    • Cong Chen
    • Bin Liu
    • Bingcheng Ren
    • Leilei Wang
    • Kai Zhao
    • Shengping Yu
    • Haolang Ming
  • View Affiliations

  • Published online on: August 20, 2013     https://doi.org/10.3892/or.2013.2669
  • Pages: 2127-2136
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Abstract

A hallmark of directional cell migration is localized actin polymerization at the leading protrusions of the cell. The Arp2/3 complex nucleates the formation of the dendritic actin network (lamellipodia) at the leading edge of motile cells. This study was designed to investigate the role of the Arp2/3 complex in the infiltrative behavior of glioma cells. Immunofluorescence and western blotting showed a positive correlation between the expression of Arp2/3 and the malignancy of glioma specimens (r=0.686, P=0.02) and confocal microscopy demonstrated localization of the Arp2/3 complex in lamellipodia of glioma cells. Furthermore, we examined the effects of Arp2/3 complex inhibition in U251, LN229 and SNB19 glioma cells using CK666, an Arp2/3 complex inhibitor. Glioma cells lost lamellipodia and cell polarity after treatment with CK666. Inhibition of the Arp2/3 complex significantly affected the ability of glioma cells to migrate and invade. In the wound-healing assay, CK666 markedly inhibited cell migration, U251 cell migration was inhibited to 38.73±3.45% of control, LN229 cells to 57.40±2.16% of control and SNB19 cells to 34.17±3.82% of control. Also, CK666 significantly impaired Transwell chamber invasion capability of U251, LN229 and SNB19 cells compared with DMSO control by 72.70±4.86, 39.12±8.42 and 41.41±4.66%, respectively. The Arp2/3 complex is, therefore, likely to be a crucial participant in glioma cell invasion and migration, and may represent a target for therapeutic intervention.
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November 2013
Volume 30 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Liu Z, Yang X, Chen C, Liu B, Ren B, Wang L, Zhao K, Yu S and Ming H: Expression of the Arp2/3 complex in human gliomas and its role in the migration and invasion of glioma cells. Oncol Rep 30: 2127-2136, 2013
APA
Liu, Z., Yang, X., Chen, C., Liu, B., Ren, B., Wang, L. ... Ming, H. (2013). Expression of the Arp2/3 complex in human gliomas and its role in the migration and invasion of glioma cells. Oncology Reports, 30, 2127-2136. https://doi.org/10.3892/or.2013.2669
MLA
Liu, Z., Yang, X., Chen, C., Liu, B., Ren, B., Wang, L., Zhao, K., Yu, S., Ming, H."Expression of the Arp2/3 complex in human gliomas and its role in the migration and invasion of glioma cells". Oncology Reports 30.5 (2013): 2127-2136.
Chicago
Liu, Z., Yang, X., Chen, C., Liu, B., Ren, B., Wang, L., Zhao, K., Yu, S., Ming, H."Expression of the Arp2/3 complex in human gliomas and its role in the migration and invasion of glioma cells". Oncology Reports 30, no. 5 (2013): 2127-2136. https://doi.org/10.3892/or.2013.2669