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Article

Enhancement of baicalin by hexamethylene bisacetamide on the induction of apoptosis contributes to simultaneous activation of the intrinsic and extrinsic apoptotic pathways in human leukemia cells

  • Authors:
    • Xia Ren
    • Yubao Zhang
    • Cuiling Li
    • Hengxiao Wang
    • Zheng Jiang
    • Zhiyong Zhang
    • Qiang Guo
    • Guanhua Song
    • Kehong Bi
    • Guosheng Jiang
  • View Affiliations / Copyright

    Affiliations: Key Laboratory for Rare and Uncommon Diseases, Key Laboratory for Tumor Immunology and Chinese Medicine Immunology of Shandong Province, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan, Shandong, P.R. China, Department of Neurosurgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, P.R. China, Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, Shandong, P.R. China, Department of Hematology, Qianfoshan Hospital, Jinan, Shandong, P.R. China
  • Pages: 2071-2080
    |
    Published online on: August 21, 2013
       https://doi.org/10.3892/or.2013.2684
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Abstract

Hexamethylene bisacetamide (HMBA) and natural flavanoid baicalin both exert potent antileukemic activity. However, there is currently no data on the anti-leukemic effects of baicalin in combination with HMBA. In the present study, we demonstrated that the combination of baicalin and HMBA synergistically inhibited the proliferation of acute myeloid leukemia (AML) cell lines. In addition, a slight G0/G1 phase arrest and significant apoptosis were observed. The combination treatment triggered apoptosis through the intrinsic pathway, which involved loss of MMP, decreased Bcl‑2/Bax ratio and Bcl‑XL/Bax ratio, caspase‑9 activation, as well as through the extrinsic pathway mediated by Fas and caspase‑8 activation. On the other hand, combination of baicalin and HMBA showed little toxic effect on peripheral blood mononuclear cells from healthy volunteers. Our results raise the possibility that the novel combination of baicalin and HMBA may be a promising regimen for the treatment of AML.
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Copy and paste a formatted citation
Spandidos Publications style
Ren X, Zhang Y, Li C, Wang H, Jiang Z, Zhang Z, Guo Q, Song G, Bi K, Jiang G, Jiang G, et al: Enhancement of baicalin by hexamethylene bisacetamide on the induction of apoptosis contributes to simultaneous activation of the intrinsic and extrinsic apoptotic pathways in human leukemia cells. Oncol Rep 30: 2071-2080, 2013.
APA
Ren, X., Zhang, Y., Li, C., Wang, H., Jiang, Z., Zhang, Z. ... Jiang, G. (2013). Enhancement of baicalin by hexamethylene bisacetamide on the induction of apoptosis contributes to simultaneous activation of the intrinsic and extrinsic apoptotic pathways in human leukemia cells. Oncology Reports, 30, 2071-2080. https://doi.org/10.3892/or.2013.2684
MLA
Ren, X., Zhang, Y., Li, C., Wang, H., Jiang, Z., Zhang, Z., Guo, Q., Song, G., Bi, K., Jiang, G."Enhancement of baicalin by hexamethylene bisacetamide on the induction of apoptosis contributes to simultaneous activation of the intrinsic and extrinsic apoptotic pathways in human leukemia cells". Oncology Reports 30.5 (2013): 2071-2080.
Chicago
Ren, X., Zhang, Y., Li, C., Wang, H., Jiang, Z., Zhang, Z., Guo, Q., Song, G., Bi, K., Jiang, G."Enhancement of baicalin by hexamethylene bisacetamide on the induction of apoptosis contributes to simultaneous activation of the intrinsic and extrinsic apoptotic pathways in human leukemia cells". Oncology Reports 30, no. 5 (2013): 2071-2080. https://doi.org/10.3892/or.2013.2684
Copy and paste a formatted citation
x
Spandidos Publications style
Ren X, Zhang Y, Li C, Wang H, Jiang Z, Zhang Z, Guo Q, Song G, Bi K, Jiang G, Jiang G, et al: Enhancement of baicalin by hexamethylene bisacetamide on the induction of apoptosis contributes to simultaneous activation of the intrinsic and extrinsic apoptotic pathways in human leukemia cells. Oncol Rep 30: 2071-2080, 2013.
APA
Ren, X., Zhang, Y., Li, C., Wang, H., Jiang, Z., Zhang, Z. ... Jiang, G. (2013). Enhancement of baicalin by hexamethylene bisacetamide on the induction of apoptosis contributes to simultaneous activation of the intrinsic and extrinsic apoptotic pathways in human leukemia cells. Oncology Reports, 30, 2071-2080. https://doi.org/10.3892/or.2013.2684
MLA
Ren, X., Zhang, Y., Li, C., Wang, H., Jiang, Z., Zhang, Z., Guo, Q., Song, G., Bi, K., Jiang, G."Enhancement of baicalin by hexamethylene bisacetamide on the induction of apoptosis contributes to simultaneous activation of the intrinsic and extrinsic apoptotic pathways in human leukemia cells". Oncology Reports 30.5 (2013): 2071-2080.
Chicago
Ren, X., Zhang, Y., Li, C., Wang, H., Jiang, Z., Zhang, Z., Guo, Q., Song, G., Bi, K., Jiang, G."Enhancement of baicalin by hexamethylene bisacetamide on the induction of apoptosis contributes to simultaneous activation of the intrinsic and extrinsic apoptotic pathways in human leukemia cells". Oncology Reports 30, no. 5 (2013): 2071-2080. https://doi.org/10.3892/or.2013.2684
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