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Article

microRNA-18b is upregulated in breast cancer and modulates genes involved in cell migration

  • Authors:
    • Miguel A. Fonseca-Sanchéz
    • Carlos Pérez-Plasencia
    • Jorge Fernández-Retana
    • Elena Arechaga-Ocampo
    • Laurence A. Marchat
    • Sergio Rodríguez-Cuevas
    • Veronica Bautista-Piña
    • Zaira E. Arellano-Anaya
    • Ali Flores-Pérez
    • José Diaz-Chávez
    • César López-Camarillo
  • View Affiliations / Copyright

    Affiliations: Oncogenomics and Cancer Proteomics Laboratory, Genomics Sciences Program, Autonomous University of Mexico City, Mexico City, Mexico, Oncogenomics Laboratory, National Institute of Cancerology, Mexico City, Mexico, Virus and Cancer Laboratory, National Institute of Cancerology, Mexico City, Mexico, Molecular Biomedicine Program and Biotechnology Network, National School of Medicine and Homeopathy, National Polytechnic Institute, Mexico City, Mexico, Institute of Breast Diseases-FUCAM, Mexico City, Mexico, Unit of Biomedical Research in Cancer, Institute of Biomedical Research, UNAM/National Cancer Institute, Mexico City, Mexico
  • Pages: 2399-2410
    |
    Published online on: August 22, 2013
       https://doi.org/10.3892/or.2013.2691
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Abstract

microRNAs are small non-coding RNAs of ~22 nucleotides that function at post-transcriptional level as negative regulators of gene expression. Aberrant expression of microRNAs could promote uncontrolled proliferation, migration and invasion of human cancer cells. In this study, we analyzed the expression of microRNA-18b (miR-18b) in breast cancer cell lines and in a set of clinical specimens. Our results showed that miR-18b was upregulated in four out of five breast cancer cell lines and also in breast tumors. In order to identify potential gene targets, we carried out transcriptional profiling of MDA-MB-231 breast cancer cells that ectopically expressed miR-18b. Our results showed that 263 genes were significantly modulated in miR-18b-deficient cells (fold change >1.5; P≤0.05). We found that knock-down of miR-18b induced the upregulation of 55 olfactory receptor (OR) genes and nine genes (NLRP7, KLK3, OLFM3, POSTN, MAGED4B, KIR3DL3, CRX, SEMG1 and CEACAM5) with key roles in cell migration and metastasis. Consistently, we found that ectopic inhibition of miR-18b suppressed the migration of two breast cancer cell models in vitro. In conclusion, we have uncovered genes directly or indirectly modulated by miR-18b which may represent potential therapeutic targets in breast cancer. Our data also pointed out a role of miR-18b in migration of breast cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Fonseca-Sanchéz MA, Pérez-Plasencia C, Fernández-Retana J, Arechaga-Ocampo E, Marchat LA, Rodríguez-Cuevas S, Bautista-Piña V, Arellano-Anaya ZE, Flores-Pérez A, Diaz-Chávez J, Diaz-Chávez J, et al: microRNA-18b is upregulated in breast cancer and modulates genes involved in cell migration. Oncol Rep 30: 2399-2410, 2013.
APA
Fonseca-Sanchéz, M.A., Pérez-Plasencia, C., Fernández-Retana, J., Arechaga-Ocampo, E., Marchat, L.A., Rodríguez-Cuevas, S. ... López-Camarillo, C. (2013). microRNA-18b is upregulated in breast cancer and modulates genes involved in cell migration. Oncology Reports, 30, 2399-2410. https://doi.org/10.3892/or.2013.2691
MLA
Fonseca-Sanchéz, M. A., Pérez-Plasencia, C., Fernández-Retana, J., Arechaga-Ocampo, E., Marchat, L. A., Rodríguez-Cuevas, S., Bautista-Piña, V., Arellano-Anaya, Z. E., Flores-Pérez, A., Diaz-Chávez, J., López-Camarillo, C."microRNA-18b is upregulated in breast cancer and modulates genes involved in cell migration". Oncology Reports 30.5 (2013): 2399-2410.
Chicago
Fonseca-Sanchéz, M. A., Pérez-Plasencia, C., Fernández-Retana, J., Arechaga-Ocampo, E., Marchat, L. A., Rodríguez-Cuevas, S., Bautista-Piña, V., Arellano-Anaya, Z. E., Flores-Pérez, A., Diaz-Chávez, J., López-Camarillo, C."microRNA-18b is upregulated in breast cancer and modulates genes involved in cell migration". Oncology Reports 30, no. 5 (2013): 2399-2410. https://doi.org/10.3892/or.2013.2691
Copy and paste a formatted citation
x
Spandidos Publications style
Fonseca-Sanchéz MA, Pérez-Plasencia C, Fernández-Retana J, Arechaga-Ocampo E, Marchat LA, Rodríguez-Cuevas S, Bautista-Piña V, Arellano-Anaya ZE, Flores-Pérez A, Diaz-Chávez J, Diaz-Chávez J, et al: microRNA-18b is upregulated in breast cancer and modulates genes involved in cell migration. Oncol Rep 30: 2399-2410, 2013.
APA
Fonseca-Sanchéz, M.A., Pérez-Plasencia, C., Fernández-Retana, J., Arechaga-Ocampo, E., Marchat, L.A., Rodríguez-Cuevas, S. ... López-Camarillo, C. (2013). microRNA-18b is upregulated in breast cancer and modulates genes involved in cell migration. Oncology Reports, 30, 2399-2410. https://doi.org/10.3892/or.2013.2691
MLA
Fonseca-Sanchéz, M. A., Pérez-Plasencia, C., Fernández-Retana, J., Arechaga-Ocampo, E., Marchat, L. A., Rodríguez-Cuevas, S., Bautista-Piña, V., Arellano-Anaya, Z. E., Flores-Pérez, A., Diaz-Chávez, J., López-Camarillo, C."microRNA-18b is upregulated in breast cancer and modulates genes involved in cell migration". Oncology Reports 30.5 (2013): 2399-2410.
Chicago
Fonseca-Sanchéz, M. A., Pérez-Plasencia, C., Fernández-Retana, J., Arechaga-Ocampo, E., Marchat, L. A., Rodríguez-Cuevas, S., Bautista-Piña, V., Arellano-Anaya, Z. E., Flores-Pérez, A., Diaz-Chávez, J., López-Camarillo, C."microRNA-18b is upregulated in breast cancer and modulates genes involved in cell migration". Oncology Reports 30, no. 5 (2013): 2399-2410. https://doi.org/10.3892/or.2013.2691
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