Myocardial protection by interferon-γ late preconditioning during cardiopulmonary bypass‑associated myocardial ischemia-reperfusion in pigs

  • Authors:
    • Xiangang Yan
    • Wanshan Qiu
    • Bing Jia
    • Hui Zhong
    • Xin Li
    • Zhanggen Chen
  • View Affiliations

  • Published online on: August 29, 2013     https://doi.org/10.3892/or.2013.2707
  • Pages: 2145-2152
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Abstract

The impact of interferon-γ (IFN-γ) late preconditioning on myocardial ischemia-reperfusion injury during cardiopulmonary bypass (CPB) and the underlying mechanism were investigated. Using a porcine model of myocardial ischemia-reperfusion injury during CPB with a 60-min aorta cross-clamp, 20 pigs (15±0.5 kg) were treated randomly with either a 1-ml (20,000 IU/kg) IFN-γ injection (IFN-γ group; n=10) or saline solution (control group; n=10) 24 h prior to CPB. Heart rate, blood pressure, left ventricular end-systolic pressure (LVESP), left ventricular end-diastolic pressure (LVEDP), creatine kinase isoenzyme-MB (CK-MB), and cardiac troponin I (cTnI) were measured before CPB, before aortic clamping, and at post-reperfusion intervals of 10, 30, 60 and 120 min. Heat shock protein 70 (HSP70), Mn-superoxide dismutase (Mn-SOD) and inducible nitric oxide synthase (iNOS) were measured by immunohistochemical staining in pre-CPB myocardial tissues. Myocardial cell apoptosis TUNEL measurement was assessed in samples obtained 60 min following reperfusion. Both groups exhibited no statistical differences in age, weight, gender and preoperative cardiac function, and worsened left ventricular function, and hemodynamic index reductions, and significant cTnI and CK-MB leakage was observed 10 and 30 min after reperfusion. At 10, 30 and 60 min following reperfusion, ventricular function and leakage of the IFN-γ group were significantly improved, and expression of HSP70, iNOS and Mn-SOD increased and myocardial cell apoptosis decreased. IFN-γ late preconditioning exhibited preventative effects on myocardial tissues in pigs during CPB surgery, likely due to increased HSP70, Mn-SOD and iNOS expression.

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November 2013
Volume 30 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yan X, Qiu W, Jia B, Zhong H, Li X and Chen Z: Myocardial protection by interferon-γ late preconditioning during cardiopulmonary bypass‑associated myocardial ischemia-reperfusion in pigs. Oncol Rep 30: 2145-2152, 2013
APA
Yan, X., Qiu, W., Jia, B., Zhong, H., Li, X., & Chen, Z. (2013). Myocardial protection by interferon-γ late preconditioning during cardiopulmonary bypass‑associated myocardial ischemia-reperfusion in pigs. Oncology Reports, 30, 2145-2152. https://doi.org/10.3892/or.2013.2707
MLA
Yan, X., Qiu, W., Jia, B., Zhong, H., Li, X., Chen, Z."Myocardial protection by interferon-γ late preconditioning during cardiopulmonary bypass‑associated myocardial ischemia-reperfusion in pigs". Oncology Reports 30.5 (2013): 2145-2152.
Chicago
Yan, X., Qiu, W., Jia, B., Zhong, H., Li, X., Chen, Z."Myocardial protection by interferon-γ late preconditioning during cardiopulmonary bypass‑associated myocardial ischemia-reperfusion in pigs". Oncology Reports 30, no. 5 (2013): 2145-2152. https://doi.org/10.3892/or.2013.2707