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International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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Article

Direct sequencing and amplification refractory mutation system for epidermal growth factor receptor mutations in patients with non-small cell lung cancer

  • Authors:
    • Huili Chu
    • Chen Zhong
    • Guoliang Xue
    • Xiuju Liang
    • Jun Wang
    • Yingxin Liu
    • Shiwei Zhao
    • Qian Zhou
    • Jingwang Bi
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, General Hospital, Jinan Command of the People's Liberation Army, Jinan, Shandong 250031, P.R. China, Department of Radiology, Jinan Central Hospital, Jinan, Shandong 250014, P.R. China
  • Pages: 2311-2315
    |
    Published online on: August 29, 2013
       https://doi.org/10.3892/or.2013.2709
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Abstract

Treatment with epidermal growth factor receptor (EGFR) tyrosine inhibitors (EGFR-TKIs) provides encouraging outcomes for advanced non-small cell lung cancer (NSCLC) patients with EGFR mutations. Pleural effusion is a common complication of NSCLC. We compared direct DNA sequencing and ADx Amplification Refractory Mutation System (ADx-ARMS) to detect EGFR mutations in malignant pleural effusion samples. We obtained 24 samples from pleural effusion fluid of NSCLC patients. Three common types of EGFR mutations were examined by direct sequencing and ADx-ARMS analysis. The sensitivity of the methods was compared and the relationship between EGFR mutations and response rates of the patients determined. In 14/24 patients, we detected EGFR mutations (58.3%) by ADx-ARMS, and in 10 samples (41.7%) by direct sequencing. In 6 samples, EGFR mutations were on exon 19, and in 8 samples, mutations were on exon 21 by ADx-ARMS. By contrast, we found EGFR mutations in 4 samples on exon 19, and in 6 samples on exon 21 by direct sequencing. Neither method showed mutations on exon 20. Among the 24 patients, there was 83.3% concordance for the methods. In 18/24 patients, gefitinib treatment was administered, including 10 patients with mutations who showed improved response compared to 8 of the wild-type patients (P<0.05). In conclusion, EGFR mutation analysis by ADx-ARMS was the most sensitive compared to direct sequencing, and provided more reliable EGFR mutation assessments. ADx-ARMS could be introduced into the clinical practice to identify NSCLC patients likely to benefit from TKI treatment, especially those with malignant pleural effusion.
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Copy and paste a formatted citation
Spandidos Publications style
Chu H, Zhong C, Xue G, Liang X, Wang J, Liu Y, Zhao S, Zhou Q and Bi J: Direct sequencing and amplification refractory mutation system for epidermal growth factor receptor mutations in patients with non-small cell lung cancer. Oncol Rep 30: 2311-2315, 2013.
APA
Chu, H., Zhong, C., Xue, G., Liang, X., Wang, J., Liu, Y. ... Bi, J. (2013). Direct sequencing and amplification refractory mutation system for epidermal growth factor receptor mutations in patients with non-small cell lung cancer. Oncology Reports, 30, 2311-2315. https://doi.org/10.3892/or.2013.2709
MLA
Chu, H., Zhong, C., Xue, G., Liang, X., Wang, J., Liu, Y., Zhao, S., Zhou, Q., Bi, J."Direct sequencing and amplification refractory mutation system for epidermal growth factor receptor mutations in patients with non-small cell lung cancer". Oncology Reports 30.5 (2013): 2311-2315.
Chicago
Chu, H., Zhong, C., Xue, G., Liang, X., Wang, J., Liu, Y., Zhao, S., Zhou, Q., Bi, J."Direct sequencing and amplification refractory mutation system for epidermal growth factor receptor mutations in patients with non-small cell lung cancer". Oncology Reports 30, no. 5 (2013): 2311-2315. https://doi.org/10.3892/or.2013.2709
Copy and paste a formatted citation
x
Spandidos Publications style
Chu H, Zhong C, Xue G, Liang X, Wang J, Liu Y, Zhao S, Zhou Q and Bi J: Direct sequencing and amplification refractory mutation system for epidermal growth factor receptor mutations in patients with non-small cell lung cancer. Oncol Rep 30: 2311-2315, 2013.
APA
Chu, H., Zhong, C., Xue, G., Liang, X., Wang, J., Liu, Y. ... Bi, J. (2013). Direct sequencing and amplification refractory mutation system for epidermal growth factor receptor mutations in patients with non-small cell lung cancer. Oncology Reports, 30, 2311-2315. https://doi.org/10.3892/or.2013.2709
MLA
Chu, H., Zhong, C., Xue, G., Liang, X., Wang, J., Liu, Y., Zhao, S., Zhou, Q., Bi, J."Direct sequencing and amplification refractory mutation system for epidermal growth factor receptor mutations in patients with non-small cell lung cancer". Oncology Reports 30.5 (2013): 2311-2315.
Chicago
Chu, H., Zhong, C., Xue, G., Liang, X., Wang, J., Liu, Y., Zhao, S., Zhou, Q., Bi, J."Direct sequencing and amplification refractory mutation system for epidermal growth factor receptor mutations in patients with non-small cell lung cancer". Oncology Reports 30, no. 5 (2013): 2311-2315. https://doi.org/10.3892/or.2013.2709
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