Promoting effects on the proliferation and metastasis of ACC tumor cell with XAGE-1b overexpression

Zhou,Bo; Li,Tingxiu; Liu,Yang; Zhu,Naishuo

doi:10.3892/or.2013.2719

Promoting effects on the proliferation and metastasis of ACC tumor cell with XAGE-1b overexpression

Authors:
- Bo Zhou
- Tingxiu Li
- Yang Liu
- Naishuo Zhu
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Affiliations: Department of Gerontology, Zhongda Hospital Affiliated to Southeast University, Nanjing 210009, P.R. China, Department of Chemotherapy, Guangxi Medical University Cancer Hospital, Nanning 530000, P.R. China, Laboratory of Molecular Virology and Immunology School of Life Sciences, Fudan University, Shanghai 200433, P.R. China
Published online on: September 5, 2013 https://doi.org/10.3892/or.2013.2719
Pages: 2323-2335

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Abstract

Adenoid cystic carcinoma is salivary gland malignancy characterized by indolent yet relentless growth that exhibits inherent resistance to surgery, chemotherapy and radiotherapy and it also expresses genes associated with well-defined carcinogenic and metastasis processes. There is no clear role established for XAGE-1b, which is a member of the cancer testis antigen family in tumorigenesis and the metastasis process of ACC. We studied and elucidated the correlation between the proliferation and metastasis of ACC and XAGE-1b. The eukaryotic vector was constructed for XAGE-1b overexpression in ACC-2 cells, which were used for studying the proliferation and migration phenotype in vivo and in vitro. RNAi technology was used to suppress the expression of XAGE-1b in the ACC-M cell line, and shRNA expression vector was also constructed and screened for interfering XAGE-1b expression applied to ACC-M cell lines. The effects on cell migration activity with XAGE-1b overexpression were determined by QCMTM 24-Well Cell Invasion Assay in vitro, and a lung metastatic model in mice. We found decreased effects on the proliferation phenotype of ACC-M cell in vivo and in vitro with XAGE-1b downregulation, and XAGE-1b overexpression promoted the proliferation of ACC-2 cells in vivo and in vitro, while its overexpression promoted the transmembrane invasion of ACC-2 cells in vitro and metastasis in vivo of the nude mice. The proliferation in vitro of ACC-M cells and subcutaneous tumor growth of nude mice was inhibited by XAGE-1b interference. The ACC-2 cell line with XAGE-1b overexpression displayed more rapid proliferation and higher transmembrane and metastatic ability in vivo and in vitro, with more angiogenesis in the tumor tissues. XAGE-1b gene was able to influence angiogenesis directly or indirectly, leading to tumorigenesis and metastasis of ACC.

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