Glucosidase II exhibits similarity to the p53 tumor suppressor in regards to structure and behavior in response to stress signals: A potential novel cancer biomarker

Suradej,Benjamart; Pata,Supansa; Kasinrerk,Watchara; Cressey,Ratchada

doi:10.3892/or.2013.2721

November 2013 Volume 30 Issue 5

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November 2013 Volume 30 Issue 5

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Article

Glucosidase II exhibits similarity to the p53 tumor suppressor in regards to structure and behavior in response to stress signals: A potential novel cancer biomarker

Authors:
- Benjamart Suradej
- Supansa Pata
- Watchara Kasinrerk
- Ratchada Cressey
View Affiliations / Copyright

Affiliations: Division of Clinical Chemistry, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand, Division of Clinical Immunology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand
Pages: 2511-2519
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Published online on: September 5, 2013

https://doi.org/10.3892/or.2013.2721
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Abstract

Early diagnosis of cancer is a key factor for the success of treatment. For this reason, identification of highly sensitive and specific novel tumor markers is urgently needed. In the present study, the CM5 polyclonal antibody (CM5 pAb) raised against p53 of mouse origin was used to identify p53 structurally related protein(s) that may also play an important role in promoting or preventing lung cancer. Western blot analysis was performed on tumor tissues and corresponding normal tissues obtained from lung cancer patients. CM5 pAb reacted with a human protein with an apparent molecular weight of 90 kDa in the lung tumor tissue. The levels of this protein were greatly increased in 35 of the 37 (94.6%) lung tumor samples assessed, with only minimal expression in the normal adjacent tissues. The 90-kDa protein was immunoprecipitated by CM5 pAb and was subsequently identified by LC-MS/MS to be glucosidase II, a key protein involved in the quality control mechanism of glycoprotein folding. An investigation of the response to genotoxic stress and endoplasmic reticulum (ER) stress using A549 human lung adenocarcinoma cells demonstrated that glucosidase II exhibited a similar pattern of response as the p53 tumor suppressor. Protein levels of both p53 and glucosidase II were increased in response to UV irradiation but decreased in response to tunicamycin-induced ER stress. In conclusion, we demonstrated that a polyclonal antibody raised against mouse p53 could cross-react with human glucosidase II, which was found to be frequently overexpressed in human lung tumor tissues and exhibited a stress response similar to p53. The high frequency of glucosidase II overexpression, which to the best of our knowledge has not been previously described, indicates its crucial roles in lung tumorigenesis and is thus a valuable biomarker for facilitating the screening and/or diagnosis of lung cancer. However, further investigations concerning its relationship to p53 and its roles in ER and genotoxic stress are warranted.

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1	Le Bras M, Delattre V, Bensaad K, Blandino G and Soussi T: Monoclonal antibodies raised against Xenopus p53 interact with human p73. Oncogene. 21:1304–1308. 2002.PubMed/NCBI
2	Harlow E, Crawford L, Pim D and Williamson N: Monoclonal antibodies specific for simian virus 40 tumor antigens. J Virol. 39:861–869. 1981.PubMed/NCBI
3	Lane DP, Stephen CW, Midgley CA, et al: Epitope analysis of the murine p53 tumour suppressor protein. Oncogene. 12:2461–2466. 1996.PubMed/NCBI
4	Midgley CA, Owens B, Briscoe CV, Thomas DB, Lane DP and Hall PA: Coupling between gamma irradiation, p53 induction and the apoptotic response depends upon cell type in vivo. J Cell Sci. 108:1843–1848. 1995.PubMed/NCBI
5	Mitprasat M, Roytrakul S, Jiemsup S, Boonseng O and Yokthongwattana K: Leaf proteomic analysis in cassava (Manihot esculenta, Crantz) during plant development, from planting of stem cutting to storage root formation. Planta. 233:1209–1221. 2011.PubMed/NCBI
6	Ruddock LW and Molinari M: N-glycan processing in ER quality control. J Cell Sci. 119:4373–4380. 2006. View Article : Google Scholar : PubMed/NCBI
7	Ellgaard L, Molinari M and Helenius A: Setting the standards: quality control in the secretory pathway. Science. 286:1882–1888. 1999. View Article : Google Scholar : PubMed/NCBI
8	Wang N, Daniels R and Hebert DN: The cotranslational maturation of the type I membrane glycoprotein tyrosinase: the heat shock protein 70 system hands off to the lectin-based chaperone system. Mol Biol Cell. 16:3740–3752. 2005. View Article : Google Scholar
9	Lee AS and Hendershot LM: ER stress and cancer. Cancer Biol Ther. 5:721–722. 2006. View Article : Google Scholar
10	Magyar JE, Gamberucci A, Konta L, et al: Endoplasmic reticulum stress underlying the pro-apoptotic effect of epigallocatechin gallate in mouse hepatoma cells. Int J Biochem Cell Biol. 41:694–700. 2009. View Article : Google Scholar : PubMed/NCBI
11	Gamberucci A, Konta L, Colucci A, et al: Green tea flavonols inhibit glucosidase II. Biochem Pharmacol. 72:640–646. 2006. View Article : Google Scholar : PubMed/NCBI
12	Ranganathan AC, Adam AP, Zhang L and Aguirre-Ghiso JA: Tumor cell dormancy induced by p38SAPK and ER-stress signaling: an adaptive advantage for metastatic cells? Cancer Biol Ther. 5:729–735. 2006. View Article : Google Scholar : PubMed/NCBI
13	Cho HY, Thomas S, Golden EB, et al: Enhanced killing of chemo-resistant breast cancer cells via controlled aggravation of ER stress. Cancer Lett. 282:87–97. 2009. View Article : Google Scholar : PubMed/NCBI
14	Jakobsen CH, Størvold GL, Bremseth H, et al: DHA induces ER stress and growth arrest in human colon cancer cells: associations with cholesterol and calcium homeostasis. J Lipid Res. 49:2089–2100. 2008. View Article : Google Scholar : PubMed/NCBI
15	Zhang LJ, Chen S, Wu P, et al: Inhibition of MEK blocks GRP78 up-regulation and enhances apoptosis induced by ER stress in gastric cancer cells. Cancer Lett. 274:40–46. 2009. View Article : Google Scholar : PubMed/NCBI
16	Elbein AD: Glycosidase inhibitors as antiviral and/or antitumor agents. Semin Cell Biol. 2:309–317. 1991.PubMed/NCBI
17	Elbein AD: Glycosidase inhibitors: inhibitors of N-linked oligosaccharide processing. FASEB J. 5:3055–3063. 1991.PubMed/NCBI
18	Horn HF and Vousden KH: Coping with stress: multiple ways to activate p53. Oncogene. 26:1306–1316. 2007. View Article : Google Scholar : PubMed/NCBI
19	Colman MS, Afshari CA and Barrett JC: Regulation of p53 stability and activity in response to genotoxic stress. Mutat Res. 462:179–188. 2000. View Article : Google Scholar : PubMed/NCBI
20	Amundson SA, Myers TG and Fornace AJ Jr: Roles for p53 in growth arrest and apoptosis: putting on the brakes after genotoxic stress. Oncogene. 17:3287–3299. 1998. View Article : Google Scholar : PubMed/NCBI
21	Pluquet O, Qu LK, Baltzis D and Koromilas AE: Endoplasmic reticulum stress accelerates p53 degradation by the cooperative actions of Hdm2 and glycogen synthase kinase 3β. Mol Cell Biol. 25:9392–9405. 2005.PubMed/NCBI
22	Qu L and Koromilas AE: Control of tumor suppressor p53 function by endoplasmic reticulum stress. Cell Cycle. 3:567–570. 2004.PubMed/NCBI
23	Stavridi ES and Halazonetis TD: p53 and stress in the ER. Genes Dev. 18:241–244. 2004. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Glucosidase II exhibits similarity to the p53 tumor suppressor in regards to structure and behavior in response to stress signals: A potential novel cancer biomarker

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