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Article

Propofol induces DNA damage in mouse leukemic monocyte macrophage RAW264.7 cells

  • Authors:
    • King-Chuen Wu
    • Su-Tso Yang
    • Shu-Chun Hsu
    • Jo-Hua Chiang
    • Te-Chun Hsia
    • Jai-Sing Yang
    • Kuo-Ching Liu
    • Rick Sai-Chuen Wu
    • Jing-Gung Chung
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, E-DA Hospital/I-Shou University, Kaohsiung 824, Taiwan, R.O.C., Department of Radiology, China Medical University Hospital, Taichung 404, Taiwan, R.O.C., Department of Biological Science and Technology, China Medical University, Taichung 404, Taiwan, R.O.C., Department of Life Sciences, National Chung Hsing University, Taichung 402, Taiwan, R.O.C., Department of Internal Medicine, China Medical University Hospital, Taichung 404, Taiwan, R.O.C., Department of Pharmacology, China Medical University, Taichung, Taiwan, R.O.C., Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 404, Taiwan, R.O.C., Department of Anesthesiology, China Medical University Hospital, Taichung 404, Taiwan, R.O.C.
  • Pages: 2304-2310
    |
    Published online on: September 5, 2013
       https://doi.org/10.3892/or.2013.2722
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Abstract

Propofol is one of the most widely clinically used intravenous anesthetic, and it induces apoptosis in human and murine leukemia cell lines. Yet, whether propofol causes DNA damage and affects the mRNA expression of repair-associated genes in cancer cells remains undetermined. In the present study, we investigated the effects of propofol on DNA damage and associated mRNA gene expression in RAW264.7 cells. Comet assay and DNA gel electrophoresis were used to evaluate DNA damage in RAW264.7 cells and propofol-inhibited cell growth in vitro. The results revealed a longer DNA tail and DNA fragmentation. Real-time PCR assay was used to examine mRNA gene expression of DNA damage and DNA repair-associated genes. Following exposure to propofol for 48 h, a decrease in the mRNA expression of DNA-PK, BRCA1, MGMT and p53 was noted in the RAW264.7 cells. Results from the western blotting indicated that p53, MGMT, 14-3-3-σ, BRCA1 and MDC1 proteins were decreased while p-p53 and p-H2A.X(S140) were increased in the RAW264.7 cells following exposure to propofol. In conclusion, exposure to propofol caused DNA damage and inhibited mRNA expression and protein levels of repair-associated genes in RAW264.7 cells.
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1 

Lichtman MA: Battling the hematological malignancies: the 200 years’ war. Oncologist. 13:126–138. 2008.PubMed/NCBI

2 

Lin JP, Yang JS, Lin JJ, et al: Rutin inhibits human leukemia tumor growth in a murine xenograft model in vivo. Environ Toxicol. 27:480–484. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Fotoohi AK, Assaraf YG, Moshfegh A, et al: Gene expression profiling of leukemia T-cells resistant to methotrexate and 7-hydroxymethotrexate reveals alterations that preserve intracellular levels of folate and nucleotide biosynthesis. Biochem Pharmacol. 77:1410–1417. 2009. View Article : Google Scholar

4 

Nau KC and Lewis WD: Multiple myeloma: diagnosis and treatment. Am Fam Physician. 78:853–859. 2008.PubMed/NCBI

5 

Nishiyama T, Matsukawa T and Hanaoka K: Intrathecal propofol has analgesic effects on inflammation-induced pain in rats. Can J Anaesth. 51:899–904. 2004. View Article : Google Scholar : PubMed/NCBI

6 

de Ruijter W, Stienen GJ, van Klarenbosch J and de Lange JJ: Negative and positive inotropic effects of propofol via L-type calcium channels and the sodium-calcium exchanger in rat cardiac trabeculae. Anesthesiology. 97:1146–1155. 2002.PubMed/NCBI

7 

Kato R and Foex P: Myocardial protection by anesthetic agents against ischemia-reperfusion injury: an update for anesthesiologists. Can J Anaesth. 49:777–791. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Ko SH, Yu CW, Lee SK, et al: Propofol attenuates ischemia-reperfusion injury in the isolated rat heart. Anesth Analg. 85:719–724. 1997.PubMed/NCBI

9 

Kokita N, Hara A, Abiko Y, Arakawa J, Hashizume H and Namiki A: Propofol improves functional and metabolic recovery in ischemic reperfused isolated rat hearts. Anesth Analg. 86:252–258. 1998.PubMed/NCBI

10 

Gao J, Zhao W, Xiang D and Shi Y: Effects of propofol on the expression of aquaporin-1 in lipopolysaccharide-activated rat lung microvessel endothelial cells. Chin J Critl Care Med. 26:670–672. 2006.

11 

Chen HI, Hsieh NK, Kao SJ and Su CF: Protective effects of propofol on acute lung injury induced by oleic acid in conscious rats. Crit Care Med. 36:1214–1221. 2008. View Article : Google Scholar : PubMed/NCBI

12 

Cui WY, Tian AY and Bai T: Protective effects of propofol on endotoxemia-induced acute kidney injury in rats. Clin Exp Pharmacol Physiol. 38:747–754. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Wu RS, Liu KC, Tang NY, et al: cDNA microarray analysis of the gene expression of murine leukemia RAW 264.7 cells after exposure to propofol. Environ Toxicol. 8:471–478. 2011.PubMed/NCBI

14 

Sagara Y, Hendler S, Khoh-Reiter S, et al: Propofol hemisuccinate protects neuronal cells from oxidative injury. J Neurochem. 73:2524–2530. 1999. View Article : Google Scholar : PubMed/NCBI

15 

Honegger P, Pardo B and Monnet-Tschudi F: Muscimol-induced death of GABAergic neurons in rat brain aggregating cell cultures. Brain Res Dev Brain Res. 105:219–225. 1998. View Article : Google Scholar : PubMed/NCBI

16 

Dallas ML, Boyle JP, Milligan CJ, et al: Carbon monoxide protects against oxidant-induced apoptosis via inhibition of Kv2.1. FASEB J. 25:1519–1530. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Lu CC, Yang JS, Huang AC, et al: Chrysophanol induces necrosis through the production of ROS and alteration of ATP levels in J5 human liver cancer cells. Mol Nutr Food Res. 54:967–976. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Chiang JH, Yang JS, Ma CY, et al: Danthron, an anthraquinone derivative, induces DNA damage and caspase cascade-mediated apoptosis in SNU-1 human gastric cancer cells through mitochondrial permeability transition pores and Bax-triggered pathways. Chem Res Toxicol. 24:20–29. 2011. View Article : Google Scholar

19 

Liu KC, Ho HC, Huang AC, et al: Gallic acid provokes DNA damage and suppresses DNA repair gene expression in human prostate cancer PC-3 cells. Environ Toxicol. Sep 2–2011.(Epub ahead of print). View Article : Google Scholar

20 

Kuo CL, Wu SY, Ip SW, et al: Apoptotic death in curcumin-treated NPC-TW 076 human nasopharyngeal carcinoma cells is mediated through the ROS, mitochondrial depolarization and caspase-3-dependent signaling responses. Int J Oncol. 39:319–328. 2011.

21 

Lu HF, Lai TY, Hsia TC, et al: Danthron induces DNA damage and inhibits DNA repair gene expressions in GBM 8401 human brain glioblastoma multiform cells. Neurochem Res. 35:1105–1110. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Yu FS, Yang JS, Yu CS, et al: Safrole induces apoptosis in human oral cancer HSC-3 cells. J Dent Res. 90:168–174. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Voso MT, D’Alo F, Greco M, et al: Epigenetic changes in therapy-related MDS/AML. Chem Biol Interact. 184:46–49. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Tsuchiya M, Asada A, Arita K, et al: Induction and mechanism of apoptotic cell death by propofol in HL-60 cells. Acta Anaesthesiol Scand. 46:1068–1074. 2002. View Article : Google Scholar : PubMed/NCBI

25 

Loeb KR and Loeb LA: Significance of multiple mutations in cancer. Carcinogenesis. 21:379–385. 2000. View Article : Google Scholar : PubMed/NCBI

26 

Ashby J, Tinwell H, Lefevre PA and Browne MA: The single cell gel electrophoresis assay for induced DNA damage (comet assay): measurement of tail length and moment. Mutagenesis. 10:85–90. 1995. View Article : Google Scholar : PubMed/NCBI

27 

Donatus IA, Sardjoko and Vermeulen NP: Cytotoxic and cytoprotective activities of curcumin. Effects on paracetamol-induced cytotoxicity, lipid peroxidation and glutathione depletion in rat hepatocytes. Biochem Pharmacol. 39:1869–1875. 1990.

28 

Pool-Zobel BL, Lotzmann N, Knoll M, et al: Detection of genotoxic effects in human gastric and nasal mucosa cells isolated from biopsy samples. Environ Mol Mutagen. 24:23–45. 1994. View Article : Google Scholar : PubMed/NCBI

29 

Olive PL, Banath JP and Durand RE: Detection of etoposide resistance by measuring DNA damage in individual Chinese hamster cells. J Natl Cancer Inst. 82:779–783. 1990. View Article : Google Scholar : PubMed/NCBI

30 

Tice RR, Andrews PW and Singh NP: The single cell gel assay: a sensitive technique for evaluating intercellular differences in DNA damage and repair. Basic Life Sci. 53:291–301. 1990.PubMed/NCBI

31 

Gulcin I, Alici HA and Cesur M: Determination of in vitro antioxidant and radical scavenging activities of propofol. Chem Pharm Bull. 53:281–285. 2005. View Article : Google Scholar : PubMed/NCBI

32 

Epe B: Role of endogenous oxidative DNA damage in carcinogenesis: what can we learn from repair-deficient mice? Biol Chem. 383:467–475. 2002.PubMed/NCBI

33 

Jiang MR, Li YC, Yang Y and Wu JR: c-Myc degradation induced by DNA damage results in apoptosis of CHO cells. Oncogene. 22:3252–3259. 2003. View Article : Google Scholar : PubMed/NCBI

34 

Marnett LJ: Oxyradicals and DNA damage. Carcinogenesis. 21:361–370. 2000. View Article : Google Scholar : PubMed/NCBI

35 

Cimprich KA and Cortez D: ATR: an essential regulator of genome integrity. Nat Rev Mol Cell Biol. 9:616–627. 2008. View Article : Google Scholar : PubMed/NCBI

36 

Liu Y and Kulesz-Martin M: p53 protein at the hub of cellular DNA damage response pathways through sequence-specific and non-sequence-specific DNA binding. Carcinogenesis. 22:851–860. 2001. View Article : Google Scholar : PubMed/NCBI

37 

Lou Z, Minter-Dykhouse K, Wu X and Chen J: MDC1 is coupled to activated CHK2 in mammalian DNA damage response pathways. Nature. 421:957–961. 2003. View Article : Google Scholar : PubMed/NCBI

38 

Choi JH, Sancar A and Lindsey-Boltz LA: The human ATR-mediated DNA damage checkpoint in a reconstituted system. Methods. 48:3–7. 2009. View Article : Google Scholar : PubMed/NCBI

39 

Venkitaraman AR: Cancer susceptibility and the functions of BRCA1 and BRCA2. Cell. 108:171–182. 2002. View Article : Google Scholar : PubMed/NCBI

40 

Jesien-Lewandowicz E, Jesionek-Kupnicka D, Zawlik I, et al: High incidence of MGMT promoter methylation in primary glioblastomas without correlation with TP53 gene mutations. Cancer Genet Cytogenet. 188:77–82. 2009.

41 

Mi J, Dziegielewski J, Bolesta E, Brautigan DL and Larner JM: Activation of DNA-PK by ionizing radiation is mediated by protein phosphatase 6. PLoS One. 4:e43952009. View Article : Google Scholar : PubMed/NCBI

42 

Braz MG, Braz LG, Mazoti MA, et al: Lower levels of oxidative DNA damage and apoptosis in lymphocytes from patients undergoing surgery with propofol anesthesia. Environ Mol Mutagen. 53:70–77. 2012. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wu K, Yang S, Hsu S, Chiang J, Hsia T, Yang J, Liu K, Wu RS and Chung J: Propofol induces DNA damage in mouse leukemic monocyte macrophage RAW264.7 cells. Oncol Rep 30: 2304-2310, 2013.
APA
Wu, K., Yang, S., Hsu, S., Chiang, J., Hsia, T., Yang, J. ... Chung, J. (2013). Propofol induces DNA damage in mouse leukemic monocyte macrophage RAW264.7 cells. Oncology Reports, 30, 2304-2310. https://doi.org/10.3892/or.2013.2722
MLA
Wu, K., Yang, S., Hsu, S., Chiang, J., Hsia, T., Yang, J., Liu, K., Wu, R. S., Chung, J."Propofol induces DNA damage in mouse leukemic monocyte macrophage RAW264.7 cells". Oncology Reports 30.5 (2013): 2304-2310.
Chicago
Wu, K., Yang, S., Hsu, S., Chiang, J., Hsia, T., Yang, J., Liu, K., Wu, R. S., Chung, J."Propofol induces DNA damage in mouse leukemic monocyte macrophage RAW264.7 cells". Oncology Reports 30, no. 5 (2013): 2304-2310. https://doi.org/10.3892/or.2013.2722
Copy and paste a formatted citation
x
Spandidos Publications style
Wu K, Yang S, Hsu S, Chiang J, Hsia T, Yang J, Liu K, Wu RS and Chung J: Propofol induces DNA damage in mouse leukemic monocyte macrophage RAW264.7 cells. Oncol Rep 30: 2304-2310, 2013.
APA
Wu, K., Yang, S., Hsu, S., Chiang, J., Hsia, T., Yang, J. ... Chung, J. (2013). Propofol induces DNA damage in mouse leukemic monocyte macrophage RAW264.7 cells. Oncology Reports, 30, 2304-2310. https://doi.org/10.3892/or.2013.2722
MLA
Wu, K., Yang, S., Hsu, S., Chiang, J., Hsia, T., Yang, J., Liu, K., Wu, R. S., Chung, J."Propofol induces DNA damage in mouse leukemic monocyte macrophage RAW264.7 cells". Oncology Reports 30.5 (2013): 2304-2310.
Chicago
Wu, K., Yang, S., Hsu, S., Chiang, J., Hsia, T., Yang, J., Liu, K., Wu, R. S., Chung, J."Propofol induces DNA damage in mouse leukemic monocyte macrophage RAW264.7 cells". Oncology Reports 30, no. 5 (2013): 2304-2310. https://doi.org/10.3892/or.2013.2722
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