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Article

Matrine induction of reactive oxygen species activates p38 leading to caspase-dependent cell apoptosis in non-small cell lung cancer cells

  • Authors:
    • Caihong Tan
    • Xiaoqiang Qian
    • Rongdi Jia
    • Min Wu
    • Zhongqin Liang
  • View Affiliations / Copyright

    Affiliations: College of Pharmacy, Soochow University, Suzhou, Jiangsu 215123, P.R. China, Department of Pharmacy, The Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, P.R. China, Department of Neurosurgery, The Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu 212001, P.R. China
  • Pages: 2529-2535
    |
    Published online on: September 9, 2013
       https://doi.org/10.3892/or.2013.2727
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Abstract

Non-small cell lung carcinoma (NSCLC) is one of the most refractory cancers in the clinic; it is insensitive to chemotherapy and is usually excised. However, screening natural compounds from herbs is also considered a possible method for its therapy. In the present study, we investigated whether matrine, a natural compound isolated from Sophora flavescens Ait. and exerting an inhibitory effect on lung cancer cells, also indicates inhibition on NSCLC cells and elucidated its molecular mechanism. Firstly, it is confirmed that matrine induces apoptosis of human NSCLC cells with anti-apoptotic factors inhibited and dependent on caspase activity. In addition, we found that matrine increases the phosphorylation of p38 but not its total protein, and inhibition of the p38 pathway with SB202190 partially prevents matrine-induced apoptosis. Furthermore, matrine generates reactive oxygen species (ROS) in a dose- and time-dependent manner, which is reversed by pretreatment with N-acetyl-L-cysteine (NAC). Additionally, inhibition of cell proliferation and increase of phosphorylation of p38 was also partially reversed by NAC. Collectively, matrine activates p38 pathway leading to a caspase-dependent apoptosis by inducing generation of ROS in NSCLC cells and may be a potential chemical for NSCLC.
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Copy and paste a formatted citation
Spandidos Publications style
Tan C, Qian X, Jia R, Wu M and Liang Z: Matrine induction of reactive oxygen species activates p38 leading to caspase-dependent cell apoptosis in non-small cell lung cancer cells. Oncol Rep 30: 2529-2535, 2013.
APA
Tan, C., Qian, X., Jia, R., Wu, M., & Liang, Z. (2013). Matrine induction of reactive oxygen species activates p38 leading to caspase-dependent cell apoptosis in non-small cell lung cancer cells. Oncology Reports, 30, 2529-2535. https://doi.org/10.3892/or.2013.2727
MLA
Tan, C., Qian, X., Jia, R., Wu, M., Liang, Z."Matrine induction of reactive oxygen species activates p38 leading to caspase-dependent cell apoptosis in non-small cell lung cancer cells". Oncology Reports 30.5 (2013): 2529-2535.
Chicago
Tan, C., Qian, X., Jia, R., Wu, M., Liang, Z."Matrine induction of reactive oxygen species activates p38 leading to caspase-dependent cell apoptosis in non-small cell lung cancer cells". Oncology Reports 30, no. 5 (2013): 2529-2535. https://doi.org/10.3892/or.2013.2727
Copy and paste a formatted citation
x
Spandidos Publications style
Tan C, Qian X, Jia R, Wu M and Liang Z: Matrine induction of reactive oxygen species activates p38 leading to caspase-dependent cell apoptosis in non-small cell lung cancer cells. Oncol Rep 30: 2529-2535, 2013.
APA
Tan, C., Qian, X., Jia, R., Wu, M., & Liang, Z. (2013). Matrine induction of reactive oxygen species activates p38 leading to caspase-dependent cell apoptosis in non-small cell lung cancer cells. Oncology Reports, 30, 2529-2535. https://doi.org/10.3892/or.2013.2727
MLA
Tan, C., Qian, X., Jia, R., Wu, M., Liang, Z."Matrine induction of reactive oxygen species activates p38 leading to caspase-dependent cell apoptosis in non-small cell lung cancer cells". Oncology Reports 30.5 (2013): 2529-2535.
Chicago
Tan, C., Qian, X., Jia, R., Wu, M., Liang, Z."Matrine induction of reactive oxygen species activates p38 leading to caspase-dependent cell apoptosis in non-small cell lung cancer cells". Oncology Reports 30, no. 5 (2013): 2529-2535. https://doi.org/10.3892/or.2013.2727
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