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Article

Antitumor effects of telomerase-specific replication-selective oncolytic viruses for adenoid cystic carcinoma cell lines

  • Authors:
    • Daisuke Sato
    • Yuji Kurihara
    • Seiji Kondo
    • Tatsuo Shirota
    • Yasuo Urata
    • Toshiyoshi Fujiwara
    • Satoru Shintani
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, Ota-ku, Tokyo 145-8515, Japan, Oncolys BioPharma, Inc., Minato-ku, Tokyo 106-0032, Japan, Division of Surgical Oncology, Department of Surgery, Okayama University, Graduate School of Medicine and Dentistry, Kita-ku, Okayama 700-8558, Japan
  • Pages: 2659-2664
    |
    Published online on: September 19, 2013
       https://doi.org/10.3892/or.2013.2738
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Abstract

We evaluated the antitumor effect of a telomerase-specific replication-selective adenovirus (Telomelysin, OBP-301) for adenoid cystic carcinoma (ACC) in vitro and in vivo. Adenovirus E1 gene expression was controlled by human telomerase reverse transcription (hTERT). Infection of ACC cells by OBP-301 induced high E1A mRNA expression and subsequent oncolytic cell death in a dose-dependent manner. Using OBP-401 (TelomeScan), a genetically engineered adenovirus that carries the GFP gene under the control of the cytomegalovirus (CMV) promoter at the deleted E3 region of OBP-301, ACC cells expressed bright GFP fluorescence as early as 12 h after OBP-401 infection. The fluorescence intensity gradually increased in a time-dependent manner, followed by rapid cell death due to the cytopathic effect of OBP-401, as evidenced by the floating, highly light-refractive cells using phase-contrast microscopy. Effects of intratumorally injected OBP-401 against established Acc2 xenograft tumors were seen in BALB/c nu/nu mice. The levels of GFP expression following ex vivo infection of OBP-401 may be of value as a positive predictive marker for the outcome of telomerase-specific virotherapy. Our data clearly indicated that telomerase-specific oncolytic adenoviruses have significant therapeutic potential against human ACC in vitro and in vivo. These results suggest that treatment with OBP-301 and OBP-401 may improve the quality of life of oral cancer patients.
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Copy and paste a formatted citation
Spandidos Publications style
Sato D, Kurihara Y, Kondo S, Shirota T, Urata Y, Fujiwara T and Shintani S: Antitumor effects of telomerase-specific replication-selective oncolytic viruses for adenoid cystic carcinoma cell lines. Oncol Rep 30: 2659-2664, 2013.
APA
Sato, D., Kurihara, Y., Kondo, S., Shirota, T., Urata, Y., Fujiwara, T., & Shintani, S. (2013). Antitumor effects of telomerase-specific replication-selective oncolytic viruses for adenoid cystic carcinoma cell lines. Oncology Reports, 30, 2659-2664. https://doi.org/10.3892/or.2013.2738
MLA
Sato, D., Kurihara, Y., Kondo, S., Shirota, T., Urata, Y., Fujiwara, T., Shintani, S."Antitumor effects of telomerase-specific replication-selective oncolytic viruses for adenoid cystic carcinoma cell lines". Oncology Reports 30.6 (2013): 2659-2664.
Chicago
Sato, D., Kurihara, Y., Kondo, S., Shirota, T., Urata, Y., Fujiwara, T., Shintani, S."Antitumor effects of telomerase-specific replication-selective oncolytic viruses for adenoid cystic carcinoma cell lines". Oncology Reports 30, no. 6 (2013): 2659-2664. https://doi.org/10.3892/or.2013.2738
Copy and paste a formatted citation
x
Spandidos Publications style
Sato D, Kurihara Y, Kondo S, Shirota T, Urata Y, Fujiwara T and Shintani S: Antitumor effects of telomerase-specific replication-selective oncolytic viruses for adenoid cystic carcinoma cell lines. Oncol Rep 30: 2659-2664, 2013.
APA
Sato, D., Kurihara, Y., Kondo, S., Shirota, T., Urata, Y., Fujiwara, T., & Shintani, S. (2013). Antitumor effects of telomerase-specific replication-selective oncolytic viruses for adenoid cystic carcinoma cell lines. Oncology Reports, 30, 2659-2664. https://doi.org/10.3892/or.2013.2738
MLA
Sato, D., Kurihara, Y., Kondo, S., Shirota, T., Urata, Y., Fujiwara, T., Shintani, S."Antitumor effects of telomerase-specific replication-selective oncolytic viruses for adenoid cystic carcinoma cell lines". Oncology Reports 30.6 (2013): 2659-2664.
Chicago
Sato, D., Kurihara, Y., Kondo, S., Shirota, T., Urata, Y., Fujiwara, T., Shintani, S."Antitumor effects of telomerase-specific replication-selective oncolytic viruses for adenoid cystic carcinoma cell lines". Oncology Reports 30, no. 6 (2013): 2659-2664. https://doi.org/10.3892/or.2013.2738
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