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Article

Valproic acid inhibits the growth of HeLa cervical cancer cells via caspase-dependent apoptosis

  • Authors:
    • Bo Ram Han
    • Bo Ra You
    • Woo Hyun Park
  • View Affiliations / Copyright

    Affiliations: Department of Physiology, Medical School, Research Institute for Endocrine Sciences, Chonbuk National University, Jeonju 561-180, Republic of Korea
  • Pages: 2999-3005
    |
    Published online on: September 20, 2013
       https://doi.org/10.3892/or.2013.2747
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Abstract

Valproic acid (VPA) as a histone deacetylase (HDAC) inhibitor has an anticancer effect. In the present study, we evaluated the effects of VPA on the growth and death of HeLa cervical cancer cells in relation to reactive oxygen species (ROS) and glutathione (GSH). Dose- and time-dependent growth inhibition was observed in HeLa cells with an IC50 of approximately 10 mM at 24 h. DNA flow cytometric analysis indicated that 10 mM VPA induced a G2/M phase arrest of the cell cycle. This agent also induced apoptosis, which was accompanied by the cleavage of PARP, the activation of caspase-3, -8 and -9, and the loss of mitochondrial membrane potential (MMP; ∆Ψm). All the tested caspase inhibitors significantly prevented HeLa apoptotic cell death induced by VPA, whereas TNF-α intensified the apoptotic cell death. With respect to ROS and GSH levels, VPA increased ROS levels and induced GSH depletion. However, N-acetyl cysteine (NAC; an antioxidant) and L-buthionine sulfoximine (BSO; a GSH synthesis inhibitor) did not significantly affect cell death in VPA-treated HeLa cells. In conclusion, VPA inhibits the growth of HeLa cervical cancer cells via caspase-dependent apoptosis and the growth inhibition is independent of ROS and GSH level changes.
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Copy and paste a formatted citation
Spandidos Publications style
Han BR, You BR and Park WH: Valproic acid inhibits the growth of HeLa cervical cancer cells via caspase-dependent apoptosis. Oncol Rep 30: 2999-3005, 2013.
APA
Han, B.R., You, B.R., & Park, W.H. (2013). Valproic acid inhibits the growth of HeLa cervical cancer cells via caspase-dependent apoptosis. Oncology Reports, 30, 2999-3005. https://doi.org/10.3892/or.2013.2747
MLA
Han, B. R., You, B. R., Park, W. H."Valproic acid inhibits the growth of HeLa cervical cancer cells via caspase-dependent apoptosis". Oncology Reports 30.6 (2013): 2999-3005.
Chicago
Han, B. R., You, B. R., Park, W. H."Valproic acid inhibits the growth of HeLa cervical cancer cells via caspase-dependent apoptosis". Oncology Reports 30, no. 6 (2013): 2999-3005. https://doi.org/10.3892/or.2013.2747
Copy and paste a formatted citation
x
Spandidos Publications style
Han BR, You BR and Park WH: Valproic acid inhibits the growth of HeLa cervical cancer cells via caspase-dependent apoptosis. Oncol Rep 30: 2999-3005, 2013.
APA
Han, B.R., You, B.R., & Park, W.H. (2013). Valproic acid inhibits the growth of HeLa cervical cancer cells via caspase-dependent apoptosis. Oncology Reports, 30, 2999-3005. https://doi.org/10.3892/or.2013.2747
MLA
Han, B. R., You, B. R., Park, W. H."Valproic acid inhibits the growth of HeLa cervical cancer cells via caspase-dependent apoptosis". Oncology Reports 30.6 (2013): 2999-3005.
Chicago
Han, B. R., You, B. R., Park, W. H."Valproic acid inhibits the growth of HeLa cervical cancer cells via caspase-dependent apoptosis". Oncology Reports 30, no. 6 (2013): 2999-3005. https://doi.org/10.3892/or.2013.2747
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