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Article

Effect of ascorbic acid and X-irradiation on HL-60 human leukemia cells: The kinetics of reactive oxygen species

  • Authors:
    • Shingo Terashima
    • Yoichiro Hosokawa
    • Hironori Yoshino
    • Masaru Yamaguchi
    • Toshiya Nakamura
  • View Affiliations / Copyright

    Affiliations: Department of Radiological Life Sciences, Division of Medical Life Sciences, Hirosaki University Graduate School of Health Sciences, Hirosaki, Aomori 036-8564, Japan, Department of Biomedical Sciences, Division of Medical Life Sciences, Hirosaki University Graduate School of Health Sciences, Hirosaki, Aomori 036-8564, Japan
  • Pages: 2653-2658
    |
    Published online on: October 1, 2013
       https://doi.org/10.3892/or.2013.2758
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Abstract

Ascorbic acid (AsA) treatment is expected to be a potential cancer therapy strategy with few side-effects that can be used alone or in combination with chemotherapy. However, the combination of AsA, a free radical scavenger, with radiation is not clearly understood; conflicting data are reported for cancer cell death. We conducted this study to determine the effect of AsA treatment combined with X-irradiation and the role of reactive oxygen species (ROS) in HL-60 human promyelocytic leukemia cells. Additive cytotoxic effects were observed when the cells were exposed to 2 Gy X-irradiation after 2.5 mM AsA treatment. When catalase was added to the culture with AsA alone, the cytotoxic effects of AsA disappeared. X-irradiation increased intercellular ROS levels and mitochondrial superoxide levels. By contrast, AsA alone and in combination with X-irradiation decreased ROS levels. However, in the presence of catalase neutralizing H2O2, AsA alone or in combination with X-irradiation only slightly decreased the intercellular ROS. Moreover, AsA decreased the mitochondrial membrane potential, which is commonly associated with apoptosis. These results suggest that the reduction of ROS did not result from ROS scavenging by AsA, and AsA induced apoptosis through a ROS-independent pathway. This study reports that a combination of AsA with radiation treatment is effective in cancer therapy when considering ROS in cancer cells.
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1 

Padayatty SJ, Sun AY, Chen Q, Espey MG, Drisko J and Levine M: Vitamin C: intravenous use by complementary and alternative medicine practitioners and adverse effects. PLoS One. 5:e114142010. View Article : Google Scholar : PubMed/NCBI

2 

Chen Q, Espey MG, Krishna MC, et al: Pharmacologic ascorbic acid concentrations selectively kill cancer cells: action as a pro-drug to deliver hydrogen peroxide to tissues. Proc Natl Acad Sci USA. 102:13604–13609. 2005. View Article : Google Scholar : PubMed/NCBI

3 

Chen Q, Espey MG, Sun AY, et al: Ascorbate in pharmacologic concentrations selectively generates ascorbate radical and hydrogen peroxide in extracellular fluid in vivo. Proc Natl Acad Sci USA. 104:8749–8787. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Takemura Y, Satoh M, Satoh K, Hamada H, Sekido Y and Kubota S: High dose of ascorbic acid induces cell death in mesothelioma cells. Biochem Biophys Res Commun. 394:249–253. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Hsieh BS, Huang LW, Su SJ, et al: Combined arginine and ascorbic acid treatment induces apoptosis in the hepatoma cell line HA22T/VGH and changes in redox status involving the pentose phosphate pathway and reactive oxygen and nitrogen species. J Nutr Biochem. 22:234–241. 2011. View Article : Google Scholar

6 

Hampton MB and Orrenius S: Dual regulation of caspase activity by hydrogen peroxide: implications for apoptosis. FEBS Lett. 414:552–556. 1997. View Article : Google Scholar : PubMed/NCBI

7 

Hyslop PA, Hinshaw DB, Halsey WA Jr, et al: Mechanisms of oxidant-mediated cell injury. The glycolytic and mitochondrial pathways of ADP phosphorylation are major intracellular targets inactivated by hydrogen peroxide. J Biol Chem. 263:1665–1675. 1998.PubMed/NCBI

8 

Lamson DW and Brignall MS: Antioxidants in cancer therapy; their actions and interactions with oncologic therapies. Altern Med Rev. 4:304–329. 1999.PubMed/NCBI

9 

Padayatty SJ, Riordan HD, Hewitt SM, Katz A, Hoffer LJ and Levine M: Intravenously administered vitamin C as cancer therapy: three cases. CMAJ. 174:937–942. 2006. View Article : Google Scholar : PubMed/NCBI

10 

Riordan HD, Casciari JJ, González MJ, et al: A pilot clinical study of continuous intravenous ascorbate in terminal cancer patients. PR Health Sci J. 24:269–276. 2005.PubMed/NCBI

11 

Monti DA, Mitchell E, Bazzan AJ, et al: Phase I evaluation of intravenous ascorbic acid in combination with gemcitabine and erlotinib in patients with metastatic pancreatic cancer. PLoS One. 7:e297942012. View Article : Google Scholar : PubMed/NCBI

12 

Shinozaki K, Hosokawa Y, Hazawa M, et al: Ascorbic acid enhances radiation-induced apoptosis in an HL60 human leukemia cell line. J Radiat Res. 52:229–237. 2011. View Article : Google Scholar : PubMed/NCBI

13 

Herst PM, Broadley KW, Harper JL and McConnell MJ: Pharmacological concentrations of ascorbate radiosensitize glioblastoma multiforme primary cells by increasing oxidative DNA damage and inhibiting G2/M arrest. Free Radic Biol Med. 52:1486–1493. 2012. View Article : Google Scholar

14 

Rose RC and Bode AM: Biology of free radical scavengers: an evaluation of ascorbate. FASEB J. 7:1135–1142. 1993.PubMed/NCBI

15 

Li Y and Schellhorn HE: New developments and novel therapeutic perspectives for vitamin C. J Nutr. 137:2171–2184. 2007.PubMed/NCBI

16 

Reth M: Antioxidant defenses - endogenous and diet derived. Free Radicals in Biology and Medicine. Halliwell B and Gutteridge JMC: 4th edition. Oxford University Press; New York: pp. 160–166. 2007

17 

Lawenda BD, Kelly KM, Ladas EJ, Sagar SM, Vickers A and Blumberg JB: Should supplemental antioxidant administration be avoided during chemotherapy and radiation therapy? J Natl Cancer Inst. 100:773–783. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Heaney ML, Gardner JR, Karasavvas N, et al: Vitamin C antagonizes the cytotoxic effects of antineoplastic drugs. Cancer Res. 68:8031–8038. 2008. View Article : Google Scholar : PubMed/NCBI

19 

Yamamoto T, Kinoshita M, Shinomiya N, et al: Pretreatment with ascorbic acid prevents lethal gastrointestinal syndrome in mice receiving a massive amount of radiation. J Radiat Res. 51:145–156. 2010. View Article : Google Scholar : PubMed/NCBI

20 

Witenberg B, Kalir HH, Raviv Z, Kletter Y, Kravtsov V and Fabian I: Inhibition by ascorbic acid of apoptosis induced by oxidative stress in HL-60 myeloid leukemia cells. Biochem Pharmacol. 57:823–832. 1999. View Article : Google Scholar : PubMed/NCBI

21 

Witenberg B, Kletter Y, Kalir HH, et al: Ascorbic acid inhibits apoptosis induced by X irradiation in HL60 myeloid leukemia cells. Radiat Res. 152:468–478. 1999. View Article : Google Scholar : PubMed/NCBI

22 

Lee YJ, Lee DH, Cho CK, et al: HSP25 inhibits radiation-induced apoptosis through reduction of PKCδ-mediated ROS production. Oncogene. 24:3715–3725. 2005.PubMed/NCBI

23 

Ni Y, Gong XG, Lu M, Chen HM and Wang Y: Mitochondrial ROS burst as an early sign in sarsasapogenin-induced apoptosis in HepG2 cells. Cell Biol Int. 32:337–343. 2008. View Article : Google Scholar : PubMed/NCBI

24 

Ogura A, Oowada S, Kon Y, et al: Redox regulation in radiation-induced cytochrome c release from mitochondria of human lung carcinoma A549 cells. Cancer Lett. 277:64–71. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Grad JM, Cepero E and Boise LH: Mitochondria as targets for established and novel anti-cancer agents. Drug Resist Updat. 4:85–91. 2001. View Article : Google Scholar : PubMed/NCBI

26 

Green DR and Reed JC: Mitochondria and apoptosis. Science. 281:1309–1312. 1998. View Article : Google Scholar : PubMed/NCBI

27 

Yoshino H, Kiminarita T, Matsushita Y and Kashiwakura I: Response of the Nrf2 protection system in human monocytic cells after ionising irradiation. Radiat Prot Dosimetry. 152:104–108. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Hachiya M and Akashi M: Catalase regulates cell growth in HL60 human promyelocytic cells: evidence for growth regulation by H2O2. Radiat Res. 163:271–282. 2005. View Article : Google Scholar : PubMed/NCBI

29 

Yanai H: Statcel-The Useful Add-in Software Forms on Excel. 2nd edition. OMS; Tokyo: 2006

30 

Koyama S, Kodama S, Suzuki K, Matsumoto T, Miyazaki T and Watanabe M: Radiation-induced long-lived radicals which cause mutation and transformation. Mutat Res. 421:45–54. 1998. View Article : Google Scholar : PubMed/NCBI

31 

Frömberg A, Gutsch D, Schulze D, et al: Ascorbate exerts anti-proliferative effects through cell cycle inhibition and sensitizes tumor cells towards cytostatic drugs. Cancer Chemother Pharmacol. 67:1157–1166. 2011.PubMed/NCBI

32 

Wang XH, Jia DZ, Liang YJ, et al: Lgf-YL-9 induces apoptosis in human epidermoid carcinoma KB cells and multidrug resistant KBv200 cells via reactive oxygen species-independent mitochondrial pathway. Cancer Lett. 249:256–270. 2007. View Article : Google Scholar

33 

Zhang JY, Wu HY, Xia XK, et al: Anthracenedione derivative 1403P-3 induces apoptosis in KB and KBv200 cells via reactive oxygen species-independent mitochondrial pathway and death receptor pathway. Cancer Biol Ther. 6:1413–1421. 2007. View Article : Google Scholar

34 

Lin KT, Xue JY, Sun FF and Wong PY: Reactive oxygen species participate in peroxynitrite-induced apoptosis in HL-60 cells. Biochem Biophys Res Commun. 230:115–119. 1997. View Article : Google Scholar : PubMed/NCBI

35 

Antunes F and Cadenas E: Estimation of H2O2 gradients across biomembranes. FEBS Lett. 475:121–126. 2000.

36 

Griffiths EJ: Mitochondria-potential role in cell life and death. Cardiovasc Res. 46:24–27. 2000. View Article : Google Scholar : PubMed/NCBI

37 

Hara K, Kasahara E, Takahashi N, et al: Mitochondria determine the efficacy of anticancer agents that interact with DNA but not the cytoskeleton. J Pharmacol Exp Ther. 337:838–845. 2011. View Article : Google Scholar : PubMed/NCBI

38 

Hou DX, Uto T, Tong X, et al: Involvement of reactive oxygen species-independent mitochondrial pathway in gossypol-induced apoptosis. Arch Biochem Biophys. 428:179–187. 2004. View Article : Google Scholar : PubMed/NCBI

39 

Ko CH, Shen SC, Hsu CS and Chen YC: Mitochondrial-dependent, reactive oxygen species-independent apoptosis by myricetin: roles of protein kinase C, cytochrome c, and caspase cascade. Biochem Pharmacol. 69:913–927. 2005. View Article : Google Scholar : PubMed/NCBI

40 

Yamamori T, Yasui H, Yamazumi M, et al: Ionizing radiation induces mitochondrial reactive oxygen species production accompanied by upregulation of mitochondrial electron transport chain function and mitochondrial content under control of the cell cycle checkpoint. Free Radic Biol Med. 53:260–270. 2012. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Terashima S, Hosokawa Y, Yoshino H, Yamaguchi M and Nakamura T: Effect of ascorbic acid and X-irradiation on HL-60 human leukemia cells: The kinetics of reactive oxygen species. Oncol Rep 30: 2653-2658, 2013.
APA
Terashima, S., Hosokawa, Y., Yoshino, H., Yamaguchi, M., & Nakamura, T. (2013). Effect of ascorbic acid and X-irradiation on HL-60 human leukemia cells: The kinetics of reactive oxygen species. Oncology Reports, 30, 2653-2658. https://doi.org/10.3892/or.2013.2758
MLA
Terashima, S., Hosokawa, Y., Yoshino, H., Yamaguchi, M., Nakamura, T."Effect of ascorbic acid and X-irradiation on HL-60 human leukemia cells: The kinetics of reactive oxygen species". Oncology Reports 30.6 (2013): 2653-2658.
Chicago
Terashima, S., Hosokawa, Y., Yoshino, H., Yamaguchi, M., Nakamura, T."Effect of ascorbic acid and X-irradiation on HL-60 human leukemia cells: The kinetics of reactive oxygen species". Oncology Reports 30, no. 6 (2013): 2653-2658. https://doi.org/10.3892/or.2013.2758
Copy and paste a formatted citation
x
Spandidos Publications style
Terashima S, Hosokawa Y, Yoshino H, Yamaguchi M and Nakamura T: Effect of ascorbic acid and X-irradiation on HL-60 human leukemia cells: The kinetics of reactive oxygen species. Oncol Rep 30: 2653-2658, 2013.
APA
Terashima, S., Hosokawa, Y., Yoshino, H., Yamaguchi, M., & Nakamura, T. (2013). Effect of ascorbic acid and X-irradiation on HL-60 human leukemia cells: The kinetics of reactive oxygen species. Oncology Reports, 30, 2653-2658. https://doi.org/10.3892/or.2013.2758
MLA
Terashima, S., Hosokawa, Y., Yoshino, H., Yamaguchi, M., Nakamura, T."Effect of ascorbic acid and X-irradiation on HL-60 human leukemia cells: The kinetics of reactive oxygen species". Oncology Reports 30.6 (2013): 2653-2658.
Chicago
Terashima, S., Hosokawa, Y., Yoshino, H., Yamaguchi, M., Nakamura, T."Effect of ascorbic acid and X-irradiation on HL-60 human leukemia cells: The kinetics of reactive oxygen species". Oncology Reports 30, no. 6 (2013): 2653-2658. https://doi.org/10.3892/or.2013.2758
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