Enhanced filopodium formation and stem-like phenotypes in a novel metastatic head and neck cancer cell model

  • Authors:
    • Yu-Syuan Chen
    • Wei-Li Huang
    • Shu-Hao Chang
    • Kuo-Wei Chang
    • Shou-Yen Kao
    • Jeng-Fan Lo
    • Pei-Fen Su
  • View Affiliations

  • Published online on: October 1, 2013     https://doi.org/10.3892/or.2013.2772
  • Pages: 2829-2837
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Abstract

Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer in the world, and metastasis is the major cause of cancer-related mortality. Prevention or elimination of metastasis may improve the survival of cancer patients; however, the availability of systemic HNSCC cell model with which to investigate the mechanisms of metastasis is limited. In the present study, we established a set of metastatic cell lines from HNSCC cells. In combination with their low-tumorigenic and high-tumorigenic ancestor cell lines, a cell model containing cell lines with varying malignant chara­cteristics was established. Transcriptome analysis revealed distinct signatures among the metastatic cell lines, in compa­rison to the ancestor cell lines. Signaling of GTPase RhoA and mammalian embryonic stem cell pluripotency were identified in the metastatic cells. Moreover, we examined the expression of genes related to epithelial-mesenchymal transition (EMT) (Snail, Slug, Twist, vimentin and fibronectin) and stemness (Oct4, Nanog and Bmi1). The capabilities of the cells for migration, invasion, spheroid formation and pulmonary colonization in nude mice were determined. Together, we demonstrated gain of Slug expression, filopodium formation and stem-like properties in metastatic HNSCC cells, rendering this model a powerful tool for the development of diagnostic biomarkers and identification of therapeutic targets for HNSCC.
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December 2013
Volume 30 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Chen Y, Huang W, Chang S, Chang K, Kao S, Lo J and Su P: Enhanced filopodium formation and stem-like phenotypes in a novel metastatic head and neck cancer cell model. Oncol Rep 30: 2829-2837, 2013
APA
Chen, Y., Huang, W., Chang, S., Chang, K., Kao, S., Lo, J., & Su, P. (2013). Enhanced filopodium formation and stem-like phenotypes in a novel metastatic head and neck cancer cell model. Oncology Reports, 30, 2829-2837. https://doi.org/10.3892/or.2013.2772
MLA
Chen, Y., Huang, W., Chang, S., Chang, K., Kao, S., Lo, J., Su, P."Enhanced filopodium formation and stem-like phenotypes in a novel metastatic head and neck cancer cell model". Oncology Reports 30.6 (2013): 2829-2837.
Chicago
Chen, Y., Huang, W., Chang, S., Chang, K., Kao, S., Lo, J., Su, P."Enhanced filopodium formation and stem-like phenotypes in a novel metastatic head and neck cancer cell model". Oncology Reports 30, no. 6 (2013): 2829-2837. https://doi.org/10.3892/or.2013.2772