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Article

Hypoxia-induced overexpression of DEC1 is regulated by HIF-1α in hepatocellular carcinoma

Retraction in: /10.3892/or.2024.8850
  • Authors:
    • Wanshan Ma
    • Xiaohong Shi
    • Sumei Lu
    • Linlin Wu
    • Yunshan Wang
  • View Affiliations / Copyright

    Affiliations: Department of Laboratory Medicine, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong 250014, P.R. China, Medical Research and Laboratory Diagnostic Center, Jinan Center Hospital Affiliated to Shandong University, Jinan, Shandong 250013, P.R. China
  • Pages: 2957-2962
    |
    Published online on: October 1, 2013
       https://doi.org/10.3892/or.2013.2774
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Abstract

Hypoxia-inducible factor-1α (HIF-1α) and differentiated embryo-chondrocyte expressed gene 1 (DEC1) are two key factors that protect hepatocellular carcinoma (HCC) cells from a hypoxic microenvironment. However, little is known concerning the effects of hypoxia on the expression of HIF-1α and DEC1 in HCC. In the present study, RT-PCR and western blotting were conducted to assay the mRNA and protein levels of HIF-1α and DEC1 under normoxia and hypoxia induced by exposure to CoCl2 for different time periods (0, 2, 4, 6, 24 and 48 h). In addition, the HIF-1α protein inhibitor, YC-1, was used to analyze the interaction between DEC1 and HIF-1α expression and the related mechanism. Results showed that expression of DEC1 in HCC was significantly upregulated at both the mRNA and protein levels, when compared with that in normal liver cells (P<0.05). Hypoxia induced the upregulation of HIF-1α in a time-dependent manner, which was also observed at the DEC1 mRNA and protein levels (P<0.05). However, hypoxia did not affect the transcription of HIF-1α (P>0.05). A positive correlation was found between HIF-1α and DEC1 expression in both BEL-7402 (r=0.885, P<0.05) and SMMC-7721 cells (r=0.826, P<0.05). Furthermore, inhibition of HIF-1α by YC-1 led to a significant decrease in DEC1 induced by hypoxia (P<0.05). We suggest that hypoxia induced the overexpression of DEC1, the mechanism of which may be related to the upregulation of HIF-1α in HCC. The efficacy of inhibiting HIF-1α and DEC1 expression as a possible treatment for HCC should be assessed in clinical trials.
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Copy and paste a formatted citation
Spandidos Publications style
Ma W, Shi X, Lu S, Wu L and Wang Y: Hypoxia-induced overexpression of DEC1 is regulated by HIF-1α in hepatocellular carcinoma Retraction in /10.3892/or.2024.8850. Oncol Rep 30: 2957-2962, 2013.
APA
Ma, W., Shi, X., Lu, S., Wu, L., & Wang, Y. (2013). Hypoxia-induced overexpression of DEC1 is regulated by HIF-1α in hepatocellular carcinoma Retraction in /10.3892/or.2024.8850. Oncology Reports, 30, 2957-2962. https://doi.org/10.3892/or.2013.2774
MLA
Ma, W., Shi, X., Lu, S., Wu, L., Wang, Y."Hypoxia-induced overexpression of DEC1 is regulated by HIF-1α in hepatocellular carcinoma Retraction in /10.3892/or.2024.8850". Oncology Reports 30.6 (2013): 2957-2962.
Chicago
Ma, W., Shi, X., Lu, S., Wu, L., Wang, Y."Hypoxia-induced overexpression of DEC1 is regulated by HIF-1α in hepatocellular carcinoma Retraction in /10.3892/or.2024.8850". Oncology Reports 30, no. 6 (2013): 2957-2962. https://doi.org/10.3892/or.2013.2774
Copy and paste a formatted citation
x
Spandidos Publications style
Ma W, Shi X, Lu S, Wu L and Wang Y: Hypoxia-induced overexpression of DEC1 is regulated by HIF-1α in hepatocellular carcinoma Retraction in /10.3892/or.2024.8850. Oncol Rep 30: 2957-2962, 2013.
APA
Ma, W., Shi, X., Lu, S., Wu, L., & Wang, Y. (2013). Hypoxia-induced overexpression of DEC1 is regulated by HIF-1α in hepatocellular carcinoma Retraction in /10.3892/or.2024.8850. Oncology Reports, 30, 2957-2962. https://doi.org/10.3892/or.2013.2774
MLA
Ma, W., Shi, X., Lu, S., Wu, L., Wang, Y."Hypoxia-induced overexpression of DEC1 is regulated by HIF-1α in hepatocellular carcinoma Retraction in /10.3892/or.2024.8850". Oncology Reports 30.6 (2013): 2957-2962.
Chicago
Ma, W., Shi, X., Lu, S., Wu, L., Wang, Y."Hypoxia-induced overexpression of DEC1 is regulated by HIF-1α in hepatocellular carcinoma Retraction in /10.3892/or.2024.8850". Oncology Reports 30, no. 6 (2013): 2957-2962. https://doi.org/10.3892/or.2013.2774
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