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Article

IQGAP1 plays an important role in the cell proliferation of multiple myeloma via the MAP kinase (ERK) pathway

Retraction in: /10.3892/or.2025.8882
  • Authors:
    • Yongyong Ma
    • Zhouxiang Jin
    • Jin Huang
    • Shujuan Zhou
    • Haige Ye
    • Songfu Jiang
    • Kang Yu
  • View Affiliations / Copyright

    Affiliations: Department of Hematology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, Department of General Surgery, Gastric Cancer Research Center, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, P.R. China, Department of Oncology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
  • Pages: 3032-3038
    |
    Published online on: October 3, 2013
       https://doi.org/10.3892/or.2013.2785
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Abstract

The present study was designed to explore the role of IQ motif-containing GTPase activating protein 1 (IQGAP1) in the cell proliferation of multiple myeloma (MM) via the MAP kinase (ERK) pathway. Reverse transcription‑polymerase chain reaction (RT-PCR) and western blot analysis were carried out to evaulate the expression of IQGAP1 in RPMI8226, U266 and KM3 cell lines and in primary MM cells from 4 MM patients. shRNA-expressing plasmids were used in RPMI8226 cells to knock down IQGAP1 and an MTT assay was used to examine the proliferative activity of the RPMI8226-shIQGAP1 (clone 1), RPMI8226-shRNA negative and untransfected RPMI8226 cells in subgroups stimulated with VEGF/IL-6 or without. Western blot analyses were then performed to examine the protein levels of p-ERK1/2, ERK1/2, AKT, p-AKT, STAT3, p-STAT3 in the RPMI8226-shIQGAP1 (clone 1), RPMI8226-shRNA negative and untransfected RPMI8226 cells. Co-immunoprecipitation was used to verify the interaction between the IQGAP1 scaffold and the MAP ERK kinase. We found that IQGAP1 was overexpressed in the human myeloma cell lines and in the patient MM cells. The proliferation rate in the RPMI8226 cells was decreased when IQGAP1 was knocked down with shRNA. IQGAP1 was found to affect RPMI8226 cell proliferation by regulation of the MAP kinase (ERK1/2) pathway; IQGAP1 scaffold-MAP kinase (ERK) interaction was noted in the human myeloma RPMI8226 cell lines. In conclusion, IQGAP1 plays an important role in the cell proliferation of MM via the MAP kinase (ERK) pathway.
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1 

Li TT, Alemayehu M, Aziziyeh AI, et al: Beta-arrestin/Ral signaling regulates lysophosphatidic acid-mediated migration and invasion of human breast tumor cells. Mol Cancer Res. 7:1064–1077. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Barlogie B, Kyle RA, Anderson KC, et al: Standard chemotherapy compared with high-dose chemoradiotherapy for multiple myeloma: final results of phase III US Intergroup Trial S9321. J Clin Oncol. 24:929–936. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Richardson P and Anderson K: Thalidomide and dexamethasone: a new standard of care for initial therapy in multiple myeloma. J Clin Oncol. 24:334–336. 2006. View Article : Google Scholar : PubMed/NCBI

4 

Richardson PG, Blood E, Mitsiades CS, et al: A randomized phase 2 study of lenalidomide therapy for patients with relapsed or relapsed and refractory multiple myeloma. Blood. 108:3458–3464. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Brown MD and Sacks DB: IQGAP1 in cellular signaling: bridging the GAP. Trends Cell Biol. 16:242–249. 2006. View Article : Google Scholar : PubMed/NCBI

6 

Jeong HW, Li Z, Brown MD and Sacks DB: IQGAP1 binds Rap1 and modulates its activity. J Biol Chem. 282:20752–20762. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Roy M, Li Z and Sacks DB: IQGAP1 binds ERK2 and modulates its activity. J Biol Chem. 279:17329–17337. 2004. View Article : Google Scholar : PubMed/NCBI

8 

Roy M, Li Z and Sacks DB: IQGAP1 is a scaffold for mitogen-activated protein kinase signaling. Mol Cell Biol. 25:9740–9752. 2005.

9 

Ren JG, Li Z and Sacks DB: IQGAP1 modulates activation of B-Raf. Proc Natl Acad Sci USA. 104:10465–10469. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Sugimoto N, Imoto I, Fukuda Y, Kurihara N, Kuroda S, Tanigami A, Kaibuchi K, Kamiyama R and Inazawa J: IQGAP1, a negative regulator of cell-cell adhesion, is upregulated by gene amplification at 15q26 in gastric cancer cell lines HSC39 and 40A. J Hum Genet. 46:21–25. 2001. View Article : Google Scholar : PubMed/NCBI

11 

Sun W, Zhang K, Zhang X, Lei W, Xiao T, Ma J, Guo S, Shao S, Zhang H, Liu Y, Yuan J, Hu Z, Ma Y, Feng X, Hu S, Zhou J, Cheng S and Gao Y: Identification of differentially expressed genes in human lung squamous cell carcinoma using suppression subtractive hybridization. Cancer Lett. 212:83–93. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Bertucci F, Salas S, Eysteries S, Nasser V, Finetti P, Ginestier C, Charafe-Jauffret E, Loriod B, Bachelart L, Montfort J, Victorero G, Viret F, Ollendorff V, Fert V, Giovaninni M, Delpero JR, Nguyen C, Viens P, Monges G, Birnbaum D and Houlgatte R: Gene expression profiling of colon cancer by DNA microarrays and correlation with histoclinical parameters. Oncogene. 23:1377–1391. 2004. View Article : Google Scholar : PubMed/NCBI

13 

Jameson KL, Mazur PK, Zehnder AM, et al: IQGAP1 scaffold-kinase interaction blockade selectively targets RAS-MAP kinase-driven tumors. Nat Med. 19:626–630. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Clark EA, Golub TR, Lander ES and Hynes RO: Genomic analysis of metastasis reveals an essential role for RhoC. Nature. 406:532–535. 2000. View Article : Google Scholar : PubMed/NCBI

15 

Nabeshima K, Shimao Y, Inoue T and Koono M: Immunohistochemical analysis of IQGAP1 expression in human colorectal carcinomas: its overexpression in carcinomas and association with invasion fronts. Cancer Lett. 176:101–109. 2002. View Article : Google Scholar : PubMed/NCBI

16 

Miyoshi T, Shirakusa T, Ishikawa Y, Iwasaki A, Shiraishi T, Makimoto Y, Iwasaki H and Nabeshima K: Possible mechanism of metastasis in lung adenocarcinomas with a micropapillary pattern. Pathol Int. 55:419–424. 2005. View Article : Google Scholar : PubMed/NCBI

17 

Dong P, Nabeshima K, Nishimura N, Kawakami T, Hachisuga T, Kawarabayashi T and Iwasaki H: Overexpression and diffuse expression pattern of IQGAP1 at invasion fronts are independent prognostic parameters in ovarian carcinomas. Cancer Lett. 243:120–127. 2006. View Article : Google Scholar : PubMed/NCBI

18 

Balenci L, Clarke ID, Dirks PB, Assard N, Ducray F, Jouvet A, Belin MF, Honnorat J and Baudier J: IQGAP1 protein specifies amplifying cancer cells in glioblastoma multiforme. Cancer Res. 66:9074–9082. 2006. View Article : Google Scholar : PubMed/NCBI

19 

White CD, Brown MD and Sacks DB: IQGAPs in cancer: a family of scaffold proteins underlying tumorigenesis. FEBS Lett. 583:1817–1824. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Scholl FA: MEK1/2 MAPK kinases are essential for mammalian development, homeostasis, and Raf-induced hyperplasia. Dev Cell. 12:615–629. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Dumesic PA, Scholl FA, Barragan DI and Khavari PA: ERK1/2 MAP kinases are required for epidermal G2/M progression. J Cell Biol. 185:409–422. 2009. View Article : Google Scholar : PubMed/NCBI

22 

McCormick F: Cancer therapy based on oncogene addiction. J Surg Oncol. 103:464–467. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Nazarian R, Shi H, Wang Q, et al: Melanomas acquire resistance to B-RAF (V600E) inhibition by RTK or N-RAS upregulation. Nature. 468:973–977. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Johannessen CM, Boehm JS, Kim SY, et al: COT drives resistance to RAF inhibition through MAP kinase pathway reactivation. Nature. 468:968–972. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Nikolaev SI, Rimoldi D, Iseli C, et al: Exome sequencing identifies recurrent somatic MAP2K1 and MAP2K2 mutations in melanoma. Nat Genet. 44:133–139. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Wagle N, Emery C, Berger MF, et al: Dissecting therapeutic resistance to RAF inhibition in melanoma by tumor genomic profiling. J Clin Oncol. 29:3085–3096. 2011. View Article : Google Scholar : PubMed/NCBI

27 

Johnson M, Sharma M and Henderson BR: IQGAP1 regulation and roles in cancer. Cell Signal. 21:1471–1478. 2009. View Article : Google Scholar

28 

White CD, Erdemir HH and Sacks DB: IQGAP1 and its binding proteins control diverse biological functions. Cell Signal. 24:826–834. 2012. View Article : Google Scholar : PubMed/NCBI

29 

Jadeski L, Mataraza JM, Jeong HW, et al: IQGAP1 stimulates proliferation and enhances tumorigenesis of human breast epithelial cells. J Biol Chem. 283:1008–1017. 2008. View Article : Google Scholar : PubMed/NCBI

30 

Liu Z, Liu D, Bojdani E, et al: IQGAP1 plays an important role in the invasiveness of thyroid cancer. Clin Cancer Res. 16:6009–6018. 2010. View Article : Google Scholar : PubMed/NCBI

31 

Sato A, Naito T, Hiramoto A, et al: Association of RNase L with a RAS GTPase-activating-like protein IQGAP1 in mediating the apoptosis of a human cancer cell-line. FEBS J. 277:4464–4473. 2010. View Article : Google Scholar : PubMed/NCBI

32 

Li S, Wang Q, Chakladar A, et al: Gastric hyperplasia in mice lacking the putative Cdc42 effector IQGAP1. Mol Cell Biol. 20:697–701. 2000. View Article : Google Scholar : PubMed/NCBI

33 

Kawano M, Hirano T, Matsuda T, et al: Autocrine generation and requirement of BSF-2/IL-6 for human multiple myelomas. Nature. 332:83–85. 1988. View Article : Google Scholar : PubMed/NCBI

34 

Pene F, Claessens Ye, Muller O, et al: Role of the phosphatidylinositol 3-kinase/Akt and mTOR/P70S6-kinase pathways in the proliferation and apoptosis in multiple myeloma. Oncogene. 21:6587–6597. 2002. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Ma Y, Jin Z, Huang J, Zhou S, Ye H, Jiang S and Yu K: IQGAP1 plays an important role in the cell proliferation of multiple myeloma via the MAP kinase (ERK) pathway Retraction in /10.3892/or.2025.8882. Oncol Rep 30: 3032-3038, 2013.
APA
Ma, Y., Jin, Z., Huang, J., Zhou, S., Ye, H., Jiang, S., & Yu, K. (2013). IQGAP1 plays an important role in the cell proliferation of multiple myeloma via the MAP kinase (ERK) pathway Retraction in /10.3892/or.2025.8882. Oncology Reports, 30, 3032-3038. https://doi.org/10.3892/or.2013.2785
MLA
Ma, Y., Jin, Z., Huang, J., Zhou, S., Ye, H., Jiang, S., Yu, K."IQGAP1 plays an important role in the cell proliferation of multiple myeloma via the MAP kinase (ERK) pathway Retraction in /10.3892/or.2025.8882". Oncology Reports 30.6 (2013): 3032-3038.
Chicago
Ma, Y., Jin, Z., Huang, J., Zhou, S., Ye, H., Jiang, S., Yu, K."IQGAP1 plays an important role in the cell proliferation of multiple myeloma via the MAP kinase (ERK) pathway Retraction in /10.3892/or.2025.8882". Oncology Reports 30, no. 6 (2013): 3032-3038. https://doi.org/10.3892/or.2013.2785
Copy and paste a formatted citation
x
Spandidos Publications style
Ma Y, Jin Z, Huang J, Zhou S, Ye H, Jiang S and Yu K: IQGAP1 plays an important role in the cell proliferation of multiple myeloma via the MAP kinase (ERK) pathway Retraction in /10.3892/or.2025.8882. Oncol Rep 30: 3032-3038, 2013.
APA
Ma, Y., Jin, Z., Huang, J., Zhou, S., Ye, H., Jiang, S., & Yu, K. (2013). IQGAP1 plays an important role in the cell proliferation of multiple myeloma via the MAP kinase (ERK) pathway Retraction in /10.3892/or.2025.8882. Oncology Reports, 30, 3032-3038. https://doi.org/10.3892/or.2013.2785
MLA
Ma, Y., Jin, Z., Huang, J., Zhou, S., Ye, H., Jiang, S., Yu, K."IQGAP1 plays an important role in the cell proliferation of multiple myeloma via the MAP kinase (ERK) pathway Retraction in /10.3892/or.2025.8882". Oncology Reports 30.6 (2013): 3032-3038.
Chicago
Ma, Y., Jin, Z., Huang, J., Zhou, S., Ye, H., Jiang, S., Yu, K."IQGAP1 plays an important role in the cell proliferation of multiple myeloma via the MAP kinase (ERK) pathway Retraction in /10.3892/or.2025.8882". Oncology Reports 30, no. 6 (2013): 3032-3038. https://doi.org/10.3892/or.2013.2785
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