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Article

Activation of estrogen receptors with E2 downregulates peroxisome proliferator-activated receptor γ in hepatocellular carcinoma

  • Authors:
    • Yueh-Min Lin
    • Bharath Kumar Velmurugan
    • Yu-Lan Yeh
    • Chuan  Chou Tu
    • Tsung-Jung Ho
    • Tung  Yuan Lai
    • Chih-Hao  Tsai
    • Fuu  Jen Tsai
    • Chang-Hai Tsai
    • Chih-Yang Huang
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Changhua Christian Hospital, Changhua, Taiwan, R.O.C., Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan, R.O.C., Institute of Medical and Molecular Toxicology, Chung Shan University, Taichung, Taiwan, R.O.C., School of Chinese Medicine, China Medical University, Taichung, Taiwan, R.O.C., Department of Traditional Medicine, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan, R.O.C., Graduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan, R.O.C., Department of Healthcare Administration, Asia University, Taichung, Taiwan, R.O.C.
  • Pages: 3027-3031
    |
    Published online on: October 10, 2013
       https://doi.org/10.3892/or.2013.2793
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Abstract

Hepatocellular carcinoma (HCC) is a leading cause of cancer-related mortality and occurs more often in men than in women; however, little is known about its underlying molecular mechanisms. The present study investigated the effect of estrogen receptor (ER)α and ERβ on peroxisome proliferator-activated receptor γ (PPARγ) expression in Hep3B cells. We examined PPARγ, ERα and ERβ mRNA and protein expression by RT-PCR and western blotting. In order to determine whether PPARγ plays a central role in HCC, we screened for PPARγ expression in liver cancer patient tissues and differentially differentiated HCC cell lines (HA22T, Huh-7, Hep3B and HepG2). We found that PPARγ expression was highly expressed in liver cancer tissues and in Hep3B cells. Furthermore, overexpression of ERα and ERβ was found to decrease PPARγ expression at the transcriptional as well as at the translational level in a ligand-dependent manner. In summary, the present study demonstrated that both ERα and β were sufficient to inhibit PPARγ and provide a valuable therapeutic option for the treatment of HCC patients.
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Copy and paste a formatted citation
Spandidos Publications style
Lin Y, Velmurugan BK, Yeh Y, Tu CC, Ho T, Lai TY, Tsai C, Tsai FJ, Tsai C, Huang C, Huang C, et al: Activation of estrogen receptors with E2 downregulates peroxisome proliferator-activated receptor γ in hepatocellular carcinoma. Oncol Rep 30: 3027-3031, 2013.
APA
Lin, Y., Velmurugan, B.K., Yeh, Y., Tu, C.C., Ho, T., Lai, T.Y. ... Huang, C. (2013). Activation of estrogen receptors with E2 downregulates peroxisome proliferator-activated receptor γ in hepatocellular carcinoma. Oncology Reports, 30, 3027-3031. https://doi.org/10.3892/or.2013.2793
MLA
Lin, Y., Velmurugan, B. K., Yeh, Y., Tu, C. C., Ho, T., Lai, T. Y., Tsai, C., Tsai, F. J., Tsai, C., Huang, C."Activation of estrogen receptors with E2 downregulates peroxisome proliferator-activated receptor γ in hepatocellular carcinoma". Oncology Reports 30.6 (2013): 3027-3031.
Chicago
Lin, Y., Velmurugan, B. K., Yeh, Y., Tu, C. C., Ho, T., Lai, T. Y., Tsai, C., Tsai, F. J., Tsai, C., Huang, C."Activation of estrogen receptors with E2 downregulates peroxisome proliferator-activated receptor γ in hepatocellular carcinoma". Oncology Reports 30, no. 6 (2013): 3027-3031. https://doi.org/10.3892/or.2013.2793
Copy and paste a formatted citation
x
Spandidos Publications style
Lin Y, Velmurugan BK, Yeh Y, Tu CC, Ho T, Lai TY, Tsai C, Tsai FJ, Tsai C, Huang C, Huang C, et al: Activation of estrogen receptors with E2 downregulates peroxisome proliferator-activated receptor γ in hepatocellular carcinoma. Oncol Rep 30: 3027-3031, 2013.
APA
Lin, Y., Velmurugan, B.K., Yeh, Y., Tu, C.C., Ho, T., Lai, T.Y. ... Huang, C. (2013). Activation of estrogen receptors with E2 downregulates peroxisome proliferator-activated receptor γ in hepatocellular carcinoma. Oncology Reports, 30, 3027-3031. https://doi.org/10.3892/or.2013.2793
MLA
Lin, Y., Velmurugan, B. K., Yeh, Y., Tu, C. C., Ho, T., Lai, T. Y., Tsai, C., Tsai, F. J., Tsai, C., Huang, C."Activation of estrogen receptors with E2 downregulates peroxisome proliferator-activated receptor γ in hepatocellular carcinoma". Oncology Reports 30.6 (2013): 3027-3031.
Chicago
Lin, Y., Velmurugan, B. K., Yeh, Y., Tu, C. C., Ho, T., Lai, T. Y., Tsai, C., Tsai, F. J., Tsai, C., Huang, C."Activation of estrogen receptors with E2 downregulates peroxisome proliferator-activated receptor γ in hepatocellular carcinoma". Oncology Reports 30, no. 6 (2013): 3027-3031. https://doi.org/10.3892/or.2013.2793
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