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Article

Aberrant O-GlcNAc-modified proteins expressed in primary colorectal cancer

  • Authors:
    • Thanong Phueaouan
    • Parunya Chaiyawat
    • Pukkavadee Netsirisawan
    • Daranee Chokchaichamnankit
    • Phaibul Punyarit
    • Chantragan Srisomsap
    • Jisnuson Svasti
    • Voraratt Champattanachai
  • View Affiliations / Copyright

    Affiliations: Applied Biological Sciences Program, Chulabhorn Graduate Institute, Bangkok, Thailand, Laboratory of Biochemistry, Chulabhorn Research Institute, Laksi, Bangkok, Thailand, Department of Clinical Pathology, Army Institute of Pathology, Pramongkutklao Medical Center, Bangkok, Thailand
  • Pages: 2929-2936
    |
    Published online on: October 11, 2013
       https://doi.org/10.3892/or.2013.2794
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Abstract

O-GlcNAcylation is a post-translational modification of serine and threonine residues which is dynamically regulated by 2 enzymes; O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) that catalyze the addition and removal of a single N-acetylglucosamine (GlcNAc) molecule, respectively. This modification is thought to be a nutrient sensor in highly proliferating cells via the hexosamine biosynthesis pathway, a minor branch of glycolysis. Although emerging evidence suggests that O-GlcNAc modification is associated with many types of cancer, identification of O-GlcNAc‑modified proteins and their role in cancer remain unexplored. In the present study, we demonstrated that O-GlcNAcylation is increased in primary colorectal cancer tissues, and that this augmentation is associated with an increased expression of OGT levels. Using 2-dimensional O-GlcNAc immunoblotting and LC-MS/MS analysis, 16 proteins were successfully identified and 8 proteins showed an increase in O-GlcNAcylation, including cytokeratin 18, heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2/B1), hnRNP H, annexin A2, annexin A7, laminin-binding protein, α-tubulin and protein DJ-1. Among these identified proteins, annexin A2 was further confirmed to show overexpression of O-GlcNAc in all cancer samples. The results, therefore, indicate that aberrant O-GlcNAcylation of proteins is associated with colorectal cancer and that identification of O-GlcNAc-modified proteins may provide novel biomarkers of cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Phueaouan T, Chaiyawat P, Netsirisawan P, Chokchaichamnankit D, Punyarit P, Srisomsap C, Svasti J and Champattanachai V: Aberrant O-GlcNAc-modified proteins expressed in primary colorectal cancer. Oncol Rep 30: 2929-2936, 2013.
APA
Phueaouan, T., Chaiyawat, P., Netsirisawan, P., Chokchaichamnankit, D., Punyarit, P., Srisomsap, C. ... Champattanachai, V. (2013). Aberrant O-GlcNAc-modified proteins expressed in primary colorectal cancer. Oncology Reports, 30, 2929-2936. https://doi.org/10.3892/or.2013.2794
MLA
Phueaouan, T., Chaiyawat, P., Netsirisawan, P., Chokchaichamnankit, D., Punyarit, P., Srisomsap, C., Svasti, J., Champattanachai, V."Aberrant O-GlcNAc-modified proteins expressed in primary colorectal cancer". Oncology Reports 30.6 (2013): 2929-2936.
Chicago
Phueaouan, T., Chaiyawat, P., Netsirisawan, P., Chokchaichamnankit, D., Punyarit, P., Srisomsap, C., Svasti, J., Champattanachai, V."Aberrant O-GlcNAc-modified proteins expressed in primary colorectal cancer". Oncology Reports 30, no. 6 (2013): 2929-2936. https://doi.org/10.3892/or.2013.2794
Copy and paste a formatted citation
x
Spandidos Publications style
Phueaouan T, Chaiyawat P, Netsirisawan P, Chokchaichamnankit D, Punyarit P, Srisomsap C, Svasti J and Champattanachai V: Aberrant O-GlcNAc-modified proteins expressed in primary colorectal cancer. Oncol Rep 30: 2929-2936, 2013.
APA
Phueaouan, T., Chaiyawat, P., Netsirisawan, P., Chokchaichamnankit, D., Punyarit, P., Srisomsap, C. ... Champattanachai, V. (2013). Aberrant O-GlcNAc-modified proteins expressed in primary colorectal cancer. Oncology Reports, 30, 2929-2936. https://doi.org/10.3892/or.2013.2794
MLA
Phueaouan, T., Chaiyawat, P., Netsirisawan, P., Chokchaichamnankit, D., Punyarit, P., Srisomsap, C., Svasti, J., Champattanachai, V."Aberrant O-GlcNAc-modified proteins expressed in primary colorectal cancer". Oncology Reports 30.6 (2013): 2929-2936.
Chicago
Phueaouan, T., Chaiyawat, P., Netsirisawan, P., Chokchaichamnankit, D., Punyarit, P., Srisomsap, C., Svasti, J., Champattanachai, V."Aberrant O-GlcNAc-modified proteins expressed in primary colorectal cancer". Oncology Reports 30, no. 6 (2013): 2929-2936. https://doi.org/10.3892/or.2013.2794
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