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Print ISSN: 1021-335X Online ISSN: 1791-2431
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2014-January Volume 31 Issue 1

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

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Article

Potential diagnostic value of miR-155 in serum from lung adenocarcinoma patients

  • Authors:
    • Feng Gao
    • Jingxia Chang
    • Huaqi Wang
    • Guojun Zhang
  • View Affiliations / Copyright

    Affiliations: Division of Respiratory and Critical Care Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China
  • Pages: 351-357
    |
    Published online on: November 1, 2013
       https://doi.org/10.3892/or.2013.2830
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Abstract

Recent studies have demonstrated that microRNAs (miRNAs) are stably detectable in plasma/serum. The expression profile of miR-21 and miR-155 was evaluated in the present study, since miR-21 is frequently reported as highly expressed in several types of cancers, while miR-155 was also found to be significantly expressed in lung cancer cell lines. Using in vitro studies, we found that miR-155 could be a candidate plasmatic biomarker for diagnosing lung cancer. We assessed the differences in levels of miR-21, miR-155, carcinoembryonic antigen (CEA) and carbohydrate antigen 125 (CA-125) expression in serum samples between lung cancer patients and healthy controls. We estimating the clinical diagnostic value of miR-155 independently and combined with CA-125 and/or CEA levels. The present study consisted of three parts: i) confirmation of the stable expression of miR-155 in the patient serum samples using quantitative PCR; ii) confirmation of higher miR-155, CEA and CA-125 levels in the patient serum samples when compared with levels in the normal controls by quantitative PCR; iii) evaluation of miR-155, CEA and CA-125 concentrations in serum sampes for tumor diagnosis of lung adenocarcinoma via ROC (receiver-operating characteristic) analysis. The results showed that i) expression of miR-155 was significantly higher in the serum of lung adenocarcinoma patients than that in normal controls (P<0.05); ii) testing results of serum miR-155 levels showed a much higher sensitivity (0.722) than that for CA-125 or CEA; iii) CEA associated with CA-125 had the highest Youden's index (0.639) in all terms of combinations; and iv) combined with CA-125 testing, miR-155 received a competitive sensitivity (0.889) and specificity (0.688) for diagnosing lung adenocarcinoma (OOP=14.88). In conclusion, endogenous miR-155 stably existed in patient serum and could be sensitively and specifically measured. Overexpression of miR-155 in serum specimens could constitute a diagnostic marker for the early detection of lung adenocarcinoma.
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Copy and paste a formatted citation
Spandidos Publications style
Gao F, Chang J, Wang H and Zhang G: Potential diagnostic value of miR-155 in serum from lung adenocarcinoma patients. Oncol Rep 31: 351-357, 2014.
APA
Gao, F., Chang, J., Wang, H., & Zhang, G. (2014). Potential diagnostic value of miR-155 in serum from lung adenocarcinoma patients. Oncology Reports, 31, 351-357. https://doi.org/10.3892/or.2013.2830
MLA
Gao, F., Chang, J., Wang, H., Zhang, G."Potential diagnostic value of miR-155 in serum from lung adenocarcinoma patients". Oncology Reports 31.1 (2014): 351-357.
Chicago
Gao, F., Chang, J., Wang, H., Zhang, G."Potential diagnostic value of miR-155 in serum from lung adenocarcinoma patients". Oncology Reports 31, no. 1 (2014): 351-357. https://doi.org/10.3892/or.2013.2830
Copy and paste a formatted citation
x
Spandidos Publications style
Gao F, Chang J, Wang H and Zhang G: Potential diagnostic value of miR-155 in serum from lung adenocarcinoma patients. Oncol Rep 31: 351-357, 2014.
APA
Gao, F., Chang, J., Wang, H., & Zhang, G. (2014). Potential diagnostic value of miR-155 in serum from lung adenocarcinoma patients. Oncology Reports, 31, 351-357. https://doi.org/10.3892/or.2013.2830
MLA
Gao, F., Chang, J., Wang, H., Zhang, G."Potential diagnostic value of miR-155 in serum from lung adenocarcinoma patients". Oncology Reports 31.1 (2014): 351-357.
Chicago
Gao, F., Chang, J., Wang, H., Zhang, G."Potential diagnostic value of miR-155 in serum from lung adenocarcinoma patients". Oncology Reports 31, no. 1 (2014): 351-357. https://doi.org/10.3892/or.2013.2830
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