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Article

A 2-nt RNA enhancer on exon 11 promotes exon 11 inclusion of the Ron proto-oncogene

  • Authors:
    • Heegyum Moon
    • Sunghee Cho
    • Tiing Jen Loh
    • Jianhua Zhou
    • Claudia Ghigna
    • Giuseppe Biamonti
    • Michael R. Green
    • Xuexiu Zheng
    • Haihong Shen
  • View Affiliations / Copyright

    Affiliations: School of life Sciences, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Nantong 226001, P.R. China, Institute of Molecular Genetics, National Research Council, I-27100 Pavia, Italy, Howard Hughes Medical Institute and Programs in Gene Function and Expression and Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA
  • Pages: 450-455
    |
    Published online on: November 5, 2013
       https://doi.org/10.3892/or.2013.2835
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Abstract

Ron is a human receptor for the macrophage-stimulating protein (MSP). Exon 11 skipping of Ron pre-mRNA produces the Ron∆165 protein that has a deletion of a 49 amino acid region in the β-chain extracellular domain. Ron∆165 is constitutively active even in the absence of its ligand. Through stepwise deletion analysis, we identified a 2-nt RNA enhancer, which is located 74 nt upstream from the 5' splice site of exon 11, for exon 11 inclusion. Through double-base and single-base substitution analysis of the 2-nt RNA, we demonstrated that the GA, CC, UG and AC dinucleotides on exon 11, in addition to the wild-type AG sequence, function as enhancers for exon 11 inclusion of the Ron pre-mRNA.
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Copy and paste a formatted citation
Spandidos Publications style
Moon H, Cho S, Loh TJ, Zhou J, Ghigna C, Biamonti G, Green MR, Zheng X and Shen H: A 2-nt RNA enhancer on exon 11 promotes exon 11 inclusion of the Ron proto-oncogene. Oncol Rep 31: 450-455, 2014.
APA
Moon, H., Cho, S., Loh, T.J., Zhou, J., Ghigna, C., Biamonti, G. ... Shen, H. (2014). A 2-nt RNA enhancer on exon 11 promotes exon 11 inclusion of the Ron proto-oncogene. Oncology Reports, 31, 450-455. https://doi.org/10.3892/or.2013.2835
MLA
Moon, H., Cho, S., Loh, T. J., Zhou, J., Ghigna, C., Biamonti, G., Green, M. R., Zheng, X., Shen, H."A 2-nt RNA enhancer on exon 11 promotes exon 11 inclusion of the Ron proto-oncogene". Oncology Reports 31.1 (2014): 450-455.
Chicago
Moon, H., Cho, S., Loh, T. J., Zhou, J., Ghigna, C., Biamonti, G., Green, M. R., Zheng, X., Shen, H."A 2-nt RNA enhancer on exon 11 promotes exon 11 inclusion of the Ron proto-oncogene". Oncology Reports 31, no. 1 (2014): 450-455. https://doi.org/10.3892/or.2013.2835
Copy and paste a formatted citation
x
Spandidos Publications style
Moon H, Cho S, Loh TJ, Zhou J, Ghigna C, Biamonti G, Green MR, Zheng X and Shen H: A 2-nt RNA enhancer on exon 11 promotes exon 11 inclusion of the Ron proto-oncogene. Oncol Rep 31: 450-455, 2014.
APA
Moon, H., Cho, S., Loh, T.J., Zhou, J., Ghigna, C., Biamonti, G. ... Shen, H. (2014). A 2-nt RNA enhancer on exon 11 promotes exon 11 inclusion of the Ron proto-oncogene. Oncology Reports, 31, 450-455. https://doi.org/10.3892/or.2013.2835
MLA
Moon, H., Cho, S., Loh, T. J., Zhou, J., Ghigna, C., Biamonti, G., Green, M. R., Zheng, X., Shen, H."A 2-nt RNA enhancer on exon 11 promotes exon 11 inclusion of the Ron proto-oncogene". Oncology Reports 31.1 (2014): 450-455.
Chicago
Moon, H., Cho, S., Loh, T. J., Zhou, J., Ghigna, C., Biamonti, G., Green, M. R., Zheng, X., Shen, H."A 2-nt RNA enhancer on exon 11 promotes exon 11 inclusion of the Ron proto-oncogene". Oncology Reports 31, no. 1 (2014): 450-455. https://doi.org/10.3892/or.2013.2835
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