Catechin-7-O-xyloside induces apoptosis via endoplasmic reticulum stress and mitochondrial dysfunction in human non-small cell lung carcinoma H1299 cells

  • Authors:
    • Jang Won Yoon
    • Jong Suk Lee
    • Byeong Mo Kim
    • Joungjwa Ahn
    • Kyung Mi Yang
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  • Published online on: November 8, 2013     https://doi.org/10.3892/or.2013.2840
  • Pages: 314-320
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Abstract

The medicinal plant Ulmus davidiana var. japonica has significant potential as a cancer chemoprevention agent. Catechin-7-O-xyloside (C7Ox) was purified from ultrafine U. davidiana var. japonica ethanol extract. In the present study, we investigated the apoptotic effect of C7Ox in the non-small cell lung cancer (NSCLC) cell line H1299. C7Ox treatment induced cell death and decreased plasma membrane integrity, an event typical of apoptosis. C7Ox-induced apoptosis was associated with the proteolytic activation of caspase-6, cleavage of poly(ADP-ribose) polymerase (PARP) and loss of mitochondrial membrane potential. C7Ox also induced the endoplasmic reticulum (ER) stress-regulated pro-apoptotic transcription factor CHOP. The suppression of CHOP expression significantly decreased C7Ox-induced cell death, LDH leakage and caspase-6 activation. Antitumor effects, evaluated based on protracted tumor regression, were observed when nude-mice bearing H1299 xenografts were treated with C7Ox. C7Ox-induced tumor regression was accompanied by enhanced expression of CHOP mRNA. Our data suggest that C7Ox can trigger mitochondrial-mediated apoptosis, and that ER stress is critical for C7Ox-induced apoptosis in H1299 NSCLC cells.
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2014-January
Volume 31 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Yoon JW, Lee JS, Kim BM, Ahn J and Yang KM: Catechin-7-O-xyloside induces apoptosis via endoplasmic reticulum stress and mitochondrial dysfunction in human non-small cell lung carcinoma H1299 cells. Oncol Rep 31: 314-320, 2014
APA
Yoon, J.W., Lee, J.S., Kim, B.M., Ahn, J., & Yang, K.M. (2014). Catechin-7-O-xyloside induces apoptosis via endoplasmic reticulum stress and mitochondrial dysfunction in human non-small cell lung carcinoma H1299 cells. Oncology Reports, 31, 314-320. https://doi.org/10.3892/or.2013.2840
MLA
Yoon, J. W., Lee, J. S., Kim, B. M., Ahn, J., Yang, K. M."Catechin-7-O-xyloside induces apoptosis via endoplasmic reticulum stress and mitochondrial dysfunction in human non-small cell lung carcinoma H1299 cells". Oncology Reports 31.1 (2014): 314-320.
Chicago
Yoon, J. W., Lee, J. S., Kim, B. M., Ahn, J., Yang, K. M."Catechin-7-O-xyloside induces apoptosis via endoplasmic reticulum stress and mitochondrial dysfunction in human non-small cell lung carcinoma H1299 cells". Oncology Reports 31, no. 1 (2014): 314-320. https://doi.org/10.3892/or.2013.2840